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Isolation and characterization of equine influenza virus (H3N8) from an equine influenza outbreak in Malaysia in 2015

Equine influenza is a major cause of respiratory infections in horses and can spread rapidly despite the availability of commercial vaccines. In this study, we carried out molecular characterization of Equine Influenza Virus (EIV) isolated from the Malaysian outbreak in 2015 by sequencing of the HA...

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Autores principales: Toh, Xinyu, Soh, Moi Lien, Ng, Mee Keun, Yap, Shew Choo, Harith, Nurshilla, Fernandez, Charlene Judith, Huangfu, Taoqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852086/
https://www.ncbi.nlm.nih.gov/pubmed/31059176
http://dx.doi.org/10.1111/tbed.13218
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author Toh, Xinyu
Soh, Moi Lien
Ng, Mee Keun
Yap, Shew Choo
Harith, Nurshilla
Fernandez, Charlene Judith
Huangfu, Taoqi
author_facet Toh, Xinyu
Soh, Moi Lien
Ng, Mee Keun
Yap, Shew Choo
Harith, Nurshilla
Fernandez, Charlene Judith
Huangfu, Taoqi
author_sort Toh, Xinyu
collection PubMed
description Equine influenza is a major cause of respiratory infections in horses and can spread rapidly despite the availability of commercial vaccines. In this study, we carried out molecular characterization of Equine Influenza Virus (EIV) isolated from the Malaysian outbreak in 2015 by sequencing of the HA and NA gene segments using Sanger sequencing. The nucleotide and amino acid sequences of HA and NA were compared with representative Florida clade 1 and clade 2 strains using phylogenetic analysis. The Florida clade 1 viruses identified in this outbreak revealed numerous amino acid substitutions in the HA protein as compared to the current OIE vaccine strain recommendations and representative strains of circulating Florida sub‐lineage clade 1 and clade 2. Differences in HA included amino acids located within antigenic sites which could lead to reduced immune recognition of the outbreak strain and alter the effectiveness of vaccination against the outbreak strain. Detailed surveillance and genetic information sharing could allow genetic drift of equine influenza viruses to be monitored more effectively on a global basis and aid in refinement of vaccine strain selection for EIV.
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spelling pubmed-68520862019-11-18 Isolation and characterization of equine influenza virus (H3N8) from an equine influenza outbreak in Malaysia in 2015 Toh, Xinyu Soh, Moi Lien Ng, Mee Keun Yap, Shew Choo Harith, Nurshilla Fernandez, Charlene Judith Huangfu, Taoqi Transbound Emerg Dis Original Articles Equine influenza is a major cause of respiratory infections in horses and can spread rapidly despite the availability of commercial vaccines. In this study, we carried out molecular characterization of Equine Influenza Virus (EIV) isolated from the Malaysian outbreak in 2015 by sequencing of the HA and NA gene segments using Sanger sequencing. The nucleotide and amino acid sequences of HA and NA were compared with representative Florida clade 1 and clade 2 strains using phylogenetic analysis. The Florida clade 1 viruses identified in this outbreak revealed numerous amino acid substitutions in the HA protein as compared to the current OIE vaccine strain recommendations and representative strains of circulating Florida sub‐lineage clade 1 and clade 2. Differences in HA included amino acids located within antigenic sites which could lead to reduced immune recognition of the outbreak strain and alter the effectiveness of vaccination against the outbreak strain. Detailed surveillance and genetic information sharing could allow genetic drift of equine influenza viruses to be monitored more effectively on a global basis and aid in refinement of vaccine strain selection for EIV. John Wiley and Sons Inc. 2019-05-22 2019-09 /pmc/articles/PMC6852086/ /pubmed/31059176 http://dx.doi.org/10.1111/tbed.13218 Text en © 2019 The Authors. Transboundary and Emerging Diseases Published by Blackwell Verlag GmbH This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Toh, Xinyu
Soh, Moi Lien
Ng, Mee Keun
Yap, Shew Choo
Harith, Nurshilla
Fernandez, Charlene Judith
Huangfu, Taoqi
Isolation and characterization of equine influenza virus (H3N8) from an equine influenza outbreak in Malaysia in 2015
title Isolation and characterization of equine influenza virus (H3N8) from an equine influenza outbreak in Malaysia in 2015
title_full Isolation and characterization of equine influenza virus (H3N8) from an equine influenza outbreak in Malaysia in 2015
title_fullStr Isolation and characterization of equine influenza virus (H3N8) from an equine influenza outbreak in Malaysia in 2015
title_full_unstemmed Isolation and characterization of equine influenza virus (H3N8) from an equine influenza outbreak in Malaysia in 2015
title_short Isolation and characterization of equine influenza virus (H3N8) from an equine influenza outbreak in Malaysia in 2015
title_sort isolation and characterization of equine influenza virus (h3n8) from an equine influenza outbreak in malaysia in 2015
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852086/
https://www.ncbi.nlm.nih.gov/pubmed/31059176
http://dx.doi.org/10.1111/tbed.13218
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