Compartments for Synthetic Cells: Osmotically Assisted Separation of Oil from Double Emulsions in a Microfluidic Chip

Liposomes are used in synthetic biology as cell‐like compartments and their microfluidic production through double emulsions allows for efficient encapsulation of various components. However, residual oil in the membrane remains a critical bottleneck for creating pristine phospholipid bilayers. It h...

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Detalles Bibliográficos
Autores principales: Krafft, Dorothee, López Castellanos, Sebastián, Lira, Rafael B., Dimova, Rumiana, Ivanov, Ivan, Sundmacher, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852271/
https://www.ncbi.nlm.nih.gov/pubmed/31090995
http://dx.doi.org/10.1002/cbic.201900152
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author Krafft, Dorothee
López Castellanos, Sebastián
Lira, Rafael B.
Dimova, Rumiana
Ivanov, Ivan
Sundmacher, Kai
author_facet Krafft, Dorothee
López Castellanos, Sebastián
Lira, Rafael B.
Dimova, Rumiana
Ivanov, Ivan
Sundmacher, Kai
author_sort Krafft, Dorothee
collection PubMed
description Liposomes are used in synthetic biology as cell‐like compartments and their microfluidic production through double emulsions allows for efficient encapsulation of various components. However, residual oil in the membrane remains a critical bottleneck for creating pristine phospholipid bilayers. It has been discovered that osmotically driven shrinking leads to detachment of the oil drop. Separation inside a microfluidic chip has been realized to automate the procedure, which allows for controlled continuous production of monodisperse liposomes.
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spelling pubmed-68522712019-11-22 Compartments for Synthetic Cells: Osmotically Assisted Separation of Oil from Double Emulsions in a Microfluidic Chip Krafft, Dorothee López Castellanos, Sebastián Lira, Rafael B. Dimova, Rumiana Ivanov, Ivan Sundmacher, Kai Chembiochem Communications Liposomes are used in synthetic biology as cell‐like compartments and their microfluidic production through double emulsions allows for efficient encapsulation of various components. However, residual oil in the membrane remains a critical bottleneck for creating pristine phospholipid bilayers. It has been discovered that osmotically driven shrinking leads to detachment of the oil drop. Separation inside a microfluidic chip has been realized to automate the procedure, which allows for controlled continuous production of monodisperse liposomes. John Wiley and Sons Inc. 2019-07-30 2019-10-15 /pmc/articles/PMC6852271/ /pubmed/31090995 http://dx.doi.org/10.1002/cbic.201900152 Text en © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Communications
Krafft, Dorothee
López Castellanos, Sebastián
Lira, Rafael B.
Dimova, Rumiana
Ivanov, Ivan
Sundmacher, Kai
Compartments for Synthetic Cells: Osmotically Assisted Separation of Oil from Double Emulsions in a Microfluidic Chip
title Compartments for Synthetic Cells: Osmotically Assisted Separation of Oil from Double Emulsions in a Microfluidic Chip
title_full Compartments for Synthetic Cells: Osmotically Assisted Separation of Oil from Double Emulsions in a Microfluidic Chip
title_fullStr Compartments for Synthetic Cells: Osmotically Assisted Separation of Oil from Double Emulsions in a Microfluidic Chip
title_full_unstemmed Compartments for Synthetic Cells: Osmotically Assisted Separation of Oil from Double Emulsions in a Microfluidic Chip
title_short Compartments for Synthetic Cells: Osmotically Assisted Separation of Oil from Double Emulsions in a Microfluidic Chip
title_sort compartments for synthetic cells: osmotically assisted separation of oil from double emulsions in a microfluidic chip
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852271/
https://www.ncbi.nlm.nih.gov/pubmed/31090995
http://dx.doi.org/10.1002/cbic.201900152
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