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Plasma microRNA-21, microRNA-146a and IL-13 expression in asthmatic children

Childhood asthma represents a worldwide problem, involving genetic, immune defense and environmental components. MicroRNAs (miRs) are non-coding, single-stranded RNAs involved in immune regulation. The aim was to evaluate clinical potential of plasma miR-21 and miR-146a involved in T helper differen...

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Autores principales: Hammad Mahmoud Hammad, Reham, Hamed, Dina Hossam El Dine, Eldosoky, Mona Abd EL Rahman, Ahmad, Ashraf Abd Elmonem Sayed, Osman, Hanan Mohsen, Abd Elgalil, Heba Mohamed, Mahmoud Hassan, Mahmoud Mohsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852388/
https://www.ncbi.nlm.nih.gov/pubmed/29635981
http://dx.doi.org/10.1177/1753425918763521
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author Hammad Mahmoud Hammad, Reham
Hamed, Dina Hossam El Dine
Eldosoky, Mona Abd EL Rahman
Ahmad, Ashraf Abd Elmonem Sayed
Osman, Hanan Mohsen
Abd Elgalil, Heba Mohamed
Mahmoud Hassan, Mahmoud Mohsen
author_facet Hammad Mahmoud Hammad, Reham
Hamed, Dina Hossam El Dine
Eldosoky, Mona Abd EL Rahman
Ahmad, Ashraf Abd Elmonem Sayed
Osman, Hanan Mohsen
Abd Elgalil, Heba Mohamed
Mahmoud Hassan, Mahmoud Mohsen
author_sort Hammad Mahmoud Hammad, Reham
collection PubMed
description Childhood asthma represents a worldwide problem, involving genetic, immune defense and environmental components. MicroRNAs (miRs) are non-coding, single-stranded RNAs involved in immune regulation. The aim was to evaluate clinical potential of plasma miR-21 and miR-146a involved in T helper differentiation in childhood asthma and non-asthmatic controls. Group 1 consisted of 27 asthmatic children receiving inhaled corticosteroids (ICSs), which was compared to group 2 with 21 healthy control children. All patients were assessed by pulmonary function tests. miR-21 and miR-146a expression levels were determined by real-time quantitative PCR, and IL-13 was measured using ELISA. Group 1 showed significant up-regulation of plasma miR-21 and miR-146a levels with mean values 42.6-fold and 4.7-fold higher than average expression, respectively, in group 2. miR-21 levels positively correlated with IL-13 levels and eosinophil percentage, while miR-146a only correlated to eosinophil percentage. There was a linear association between each of miR-21 and miR-146a expression and FEV1 (forced expiratory volume in the first second), miR-21 and miR-146a are up-regulated in asthmatic children. miR-21 served as a better asthma biomarker. Association between both markers and FEV1 points to their role in determining asthma outcome following ICS treatment. miR-21 and miR-146a play a role in eosinophilic endotypic classification of asthma.
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spelling pubmed-68523882019-11-20 Plasma microRNA-21, microRNA-146a and IL-13 expression in asthmatic children Hammad Mahmoud Hammad, Reham Hamed, Dina Hossam El Dine Eldosoky, Mona Abd EL Rahman Ahmad, Ashraf Abd Elmonem Sayed Osman, Hanan Mohsen Abd Elgalil, Heba Mohamed Mahmoud Hassan, Mahmoud Mohsen Innate Immun Original Articles Childhood asthma represents a worldwide problem, involving genetic, immune defense and environmental components. MicroRNAs (miRs) are non-coding, single-stranded RNAs involved in immune regulation. The aim was to evaluate clinical potential of plasma miR-21 and miR-146a involved in T helper differentiation in childhood asthma and non-asthmatic controls. Group 1 consisted of 27 asthmatic children receiving inhaled corticosteroids (ICSs), which was compared to group 2 with 21 healthy control children. All patients were assessed by pulmonary function tests. miR-21 and miR-146a expression levels were determined by real-time quantitative PCR, and IL-13 was measured using ELISA. Group 1 showed significant up-regulation of plasma miR-21 and miR-146a levels with mean values 42.6-fold and 4.7-fold higher than average expression, respectively, in group 2. miR-21 levels positively correlated with IL-13 levels and eosinophil percentage, while miR-146a only correlated to eosinophil percentage. There was a linear association between each of miR-21 and miR-146a expression and FEV1 (forced expiratory volume in the first second), miR-21 and miR-146a are up-regulated in asthmatic children. miR-21 served as a better asthma biomarker. Association between both markers and FEV1 points to their role in determining asthma outcome following ICS treatment. miR-21 and miR-146a play a role in eosinophilic endotypic classification of asthma. SAGE Publications 2018-04-10 2018-04 /pmc/articles/PMC6852388/ /pubmed/29635981 http://dx.doi.org/10.1177/1753425918763521 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Hammad Mahmoud Hammad, Reham
Hamed, Dina Hossam El Dine
Eldosoky, Mona Abd EL Rahman
Ahmad, Ashraf Abd Elmonem Sayed
Osman, Hanan Mohsen
Abd Elgalil, Heba Mohamed
Mahmoud Hassan, Mahmoud Mohsen
Plasma microRNA-21, microRNA-146a and IL-13 expression in asthmatic children
title Plasma microRNA-21, microRNA-146a and IL-13 expression in asthmatic children
title_full Plasma microRNA-21, microRNA-146a and IL-13 expression in asthmatic children
title_fullStr Plasma microRNA-21, microRNA-146a and IL-13 expression in asthmatic children
title_full_unstemmed Plasma microRNA-21, microRNA-146a and IL-13 expression in asthmatic children
title_short Plasma microRNA-21, microRNA-146a and IL-13 expression in asthmatic children
title_sort plasma microrna-21, microrna-146a and il-13 expression in asthmatic children
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852388/
https://www.ncbi.nlm.nih.gov/pubmed/29635981
http://dx.doi.org/10.1177/1753425918763521
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