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Microbial co-infection alters macrophage polarization, phagosomal escape, and microbial killing
Macrophages are important innate immune cells that respond to microbial insults. In response to multi-bacterial infection, the macrophage activation state may change upon exposure to nascent mediators, which results in different bacterial killing mechanism(s). In this study, we utilized two respirat...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852389/ https://www.ncbi.nlm.nih.gov/pubmed/29482417 http://dx.doi.org/10.1177/1753425918760180 |
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author | Trivedi, Nikita H Yu, Jieh-Juen Hung, Chiung-Yu Doelger, Richard P Navara, Christopher S Armitige, Lisa Y Seshu, Janakiram Sinai, Anthony P Chambers, James P Guentzel, M Neal Arulanandam, Bernard P |
author_facet | Trivedi, Nikita H Yu, Jieh-Juen Hung, Chiung-Yu Doelger, Richard P Navara, Christopher S Armitige, Lisa Y Seshu, Janakiram Sinai, Anthony P Chambers, James P Guentzel, M Neal Arulanandam, Bernard P |
author_sort | Trivedi, Nikita H |
collection | PubMed |
description | Macrophages are important innate immune cells that respond to microbial insults. In response to multi-bacterial infection, the macrophage activation state may change upon exposure to nascent mediators, which results in different bacterial killing mechanism(s). In this study, we utilized two respiratory bacterial pathogens, Mycobacterium bovis (Bacillus Calmette Guẻrin, BCG) and Francisella tularensis live vaccine strain (LVS) with different phagocyte evasion mechanisms, as model microbes to assess the influence of initial bacterial infection on the macrophage response to secondary infection. Non-activated (M0) macrophages or activated M2-polarized cells (J774 cells transfected with the mouse IL-4 gene) were first infected with BCG for 24–48 h, subsequently challenged with LVS, and the results of inhibition of LVS replication in the macrophages was assessed. BCG infection in M0 macrophages activated TLR2-MyD88 and Mincle-CARD9 signaling pathways, stimulating nitric oxide (NO) production and enhanced killing of LVS. BCG infection had little effect on LVS escape from phagosomes into the cytosol in M0 macrophages. In contrast, M2-polarized macrophages exhibited enhanced endosomal acidification, as well as inhibiting LVS replication. Pre-infection with BCG did not induce NO production and thus did not further reduce LVS replication. This study provides a model for studies of the complexity of macrophage activation in response to multi-bacterial infection. |
format | Online Article Text |
id | pubmed-6852389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-68523892019-11-20 Microbial co-infection alters macrophage polarization, phagosomal escape, and microbial killing Trivedi, Nikita H Yu, Jieh-Juen Hung, Chiung-Yu Doelger, Richard P Navara, Christopher S Armitige, Lisa Y Seshu, Janakiram Sinai, Anthony P Chambers, James P Guentzel, M Neal Arulanandam, Bernard P Innate Immun Original Articles Macrophages are important innate immune cells that respond to microbial insults. In response to multi-bacterial infection, the macrophage activation state may change upon exposure to nascent mediators, which results in different bacterial killing mechanism(s). In this study, we utilized two respiratory bacterial pathogens, Mycobacterium bovis (Bacillus Calmette Guẻrin, BCG) and Francisella tularensis live vaccine strain (LVS) with different phagocyte evasion mechanisms, as model microbes to assess the influence of initial bacterial infection on the macrophage response to secondary infection. Non-activated (M0) macrophages or activated M2-polarized cells (J774 cells transfected with the mouse IL-4 gene) were first infected with BCG for 24–48 h, subsequently challenged with LVS, and the results of inhibition of LVS replication in the macrophages was assessed. BCG infection in M0 macrophages activated TLR2-MyD88 and Mincle-CARD9 signaling pathways, stimulating nitric oxide (NO) production and enhanced killing of LVS. BCG infection had little effect on LVS escape from phagosomes into the cytosol in M0 macrophages. In contrast, M2-polarized macrophages exhibited enhanced endosomal acidification, as well as inhibiting LVS replication. Pre-infection with BCG did not induce NO production and thus did not further reduce LVS replication. This study provides a model for studies of the complexity of macrophage activation in response to multi-bacterial infection. SAGE Publications 2018-02-26 2018-04 /pmc/articles/PMC6852389/ /pubmed/29482417 http://dx.doi.org/10.1177/1753425918760180 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Articles Trivedi, Nikita H Yu, Jieh-Juen Hung, Chiung-Yu Doelger, Richard P Navara, Christopher S Armitige, Lisa Y Seshu, Janakiram Sinai, Anthony P Chambers, James P Guentzel, M Neal Arulanandam, Bernard P Microbial co-infection alters macrophage polarization, phagosomal escape, and microbial killing |
title | Microbial co-infection alters macrophage polarization, phagosomal
escape, and microbial killing |
title_full | Microbial co-infection alters macrophage polarization, phagosomal
escape, and microbial killing |
title_fullStr | Microbial co-infection alters macrophage polarization, phagosomal
escape, and microbial killing |
title_full_unstemmed | Microbial co-infection alters macrophage polarization, phagosomal
escape, and microbial killing |
title_short | Microbial co-infection alters macrophage polarization, phagosomal
escape, and microbial killing |
title_sort | microbial co-infection alters macrophage polarization, phagosomal
escape, and microbial killing |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852389/ https://www.ncbi.nlm.nih.gov/pubmed/29482417 http://dx.doi.org/10.1177/1753425918760180 |
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