Gene modification by fast‐track recombineering for cellular localization and isolation of components of plant protein complexes

To accelerate the isolation of plant protein complexes and study cellular localization and interaction of their components, an improved recombineering protocol is described for simple and fast site‐directed modification of plant genes in bacterial artificial chromosomes (BACs). Coding sequences of f...

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Detalles Bibliográficos
Autores principales: Hu, Zhoubo, Ghosh, Ajit, Stolze, Sara C., Horváth, Mihály, Bai, Bing, Schaefer, Sabine, Zündorf, Simone, Liu, Shanda, Harzen, Anne, Hajheidari, Mohsen, Sarnowski, Tomasz J., Nakagami, Hirofumi, Koncz, Zsuzsa, Koncz, Csaba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Technical Advance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852550/
https://www.ncbi.nlm.nih.gov/pubmed/31276249
http://dx.doi.org/10.1111/tpj.14450

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