In vivo monitoring of hepatic glycolipid distribution of n-6 ∕ n-3 in jugular-vein-cannulated rats as a nutritional research model for monogastric animal

The metabolic distribution via blood from liver of glycerolipids by omega-6 to omega-3 fatty acid ([Formula: see text]-6 [Formula: see text] [Formula: see text]-3) ratio in monogastric animal nutrition is very important. In vivo monitoring technique using jugular-vein-cannulated rats as a nutritional model for monogastric animal can yield important insights into animal nutrition. This study was conducted to determine the effect of different [Formula: see text]-6 [Formula: see text] [Formula: see text]-3 ratios ([Formula: see text] , [Formula: see text] , [Formula: see text] , [Formula: see text]) on metabolic distribution of glycerolipids newly synthesized and secreted in the liver of the rats and explore the mechanism involved. Regarding [Formula: see text] released from oxidation of glycerolipid metabolism, it was the highest (22.5 %) in groups with a [Formula: see text]-6 [Formula: see text] [Formula: see text]-3 ratio of [Formula: see text] ([Formula: see text]). The control group showed the highest total glycerolipid level, followed by the [Formula: see text] , [Formula: see text] , and [Formula: see text] groups in order ([Formula: see text]). When secreted triacylglycerol level of each group was compared with that of the control group, the [Formula: see text] , [Formula: see text] , and [Formula: see text] groups were decreased by 36.3 %, 20.9 %, and 13.3 %, respectively ([Formula: see text]). Regarding the distribution of phospholipid against total glycerolipid compared to the control group, the [Formula: see text] , [Formula: see text] , and [Formula: see text] groups were 1.38, 1.29, and 1.17 times higher, respectively ([Formula: see text]). In the comparison of [Formula: see text] emission against total glycerolipid compared with the control group, the [Formula: see text] , [Formula: see text] , and [Formula: see text] groups were 1.61, 1.52, and 1.29 times higher, respectively ([Formula: see text]). These results demonstrate that a dietary [Formula: see text]-6 [Formula: see text] [Formula: see text]-3 fatty acid ratio of [Formula: see text] could significantly decrease harmful lipid levels in the blood by controlling the mechanism of metabolic distribution via blood from triglyceride and phospholipid newly synthesized in the liver of cannulated rat.