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Preliminary In Vitro Effects of CD8+ T Lymphocyte Specific for the CD20 Alternative Splicing D393-CD20 Peptide Expressed on Burkitt Lymphoma Cells

The effective discovery of clinically relevant tumor antigens holds a fundamental role for the development of new diagnostic tools and anticancer immunotherapies. D393-CD20 mRNA is absent from normal resting B cells but present in various malignant or transformed B cells. CD8+T lymphocytes play a ce...

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Autores principales: Chegni, Hamid, Hassan, Zuhair M, Nisini, Roberto, Ebrahimi, Marzieh, Sabouni, Farzaneh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852797/
https://www.ncbi.nlm.nih.gov/pubmed/31450932
http://dx.doi.org/10.31557/APJCP.2019.20.8.2563
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author Chegni, Hamid
Hassan, Zuhair M
Nisini, Roberto
Ebrahimi, Marzieh
Sabouni, Farzaneh
author_facet Chegni, Hamid
Hassan, Zuhair M
Nisini, Roberto
Ebrahimi, Marzieh
Sabouni, Farzaneh
author_sort Chegni, Hamid
collection PubMed
description The effective discovery of clinically relevant tumor antigens holds a fundamental role for the development of new diagnostic tools and anticancer immunotherapies. D393-CD20 mRNA is absent from normal resting B cells but present in various malignant or transformed B cells. CD8+T lymphocytes play a central role in immunity to cancer. In this study, we want use from T CD8+ against D393-CD20 for effect in RAMOS cell line. After isolation and expanding of specific TCD8 + Lymphocyte against D393-CD20 antigen, for examining the effect of specialized T lymphocyte clone of D393-CD20 antigen on RAMOS cell line, we co-cultured them together, and the rate of apoptosis were examined by flow cytometry and cytotoxicity techniques by using MTT technique. We observed that specialized TCD8+ lymphocyte of D393-CD20 antigen can induce apoptosis in malignant B-lymphocytes, and this antigen can be a proper target for immunotherapy.
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spelling pubmed-68527972019-12-12 Preliminary In Vitro Effects of CD8+ T Lymphocyte Specific for the CD20 Alternative Splicing D393-CD20 Peptide Expressed on Burkitt Lymphoma Cells Chegni, Hamid Hassan, Zuhair M Nisini, Roberto Ebrahimi, Marzieh Sabouni, Farzaneh Asian Pac J Cancer Prev Research Article The effective discovery of clinically relevant tumor antigens holds a fundamental role for the development of new diagnostic tools and anticancer immunotherapies. D393-CD20 mRNA is absent from normal resting B cells but present in various malignant or transformed B cells. CD8+T lymphocytes play a central role in immunity to cancer. In this study, we want use from T CD8+ against D393-CD20 for effect in RAMOS cell line. After isolation and expanding of specific TCD8 + Lymphocyte against D393-CD20 antigen, for examining the effect of specialized T lymphocyte clone of D393-CD20 antigen on RAMOS cell line, we co-cultured them together, and the rate of apoptosis were examined by flow cytometry and cytotoxicity techniques by using MTT technique. We observed that specialized TCD8+ lymphocyte of D393-CD20 antigen can induce apoptosis in malignant B-lymphocytes, and this antigen can be a proper target for immunotherapy. West Asia Organization for Cancer Prevention 2019 /pmc/articles/PMC6852797/ /pubmed/31450932 http://dx.doi.org/10.31557/APJCP.2019.20.8.2563 Text en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle Research Article
Chegni, Hamid
Hassan, Zuhair M
Nisini, Roberto
Ebrahimi, Marzieh
Sabouni, Farzaneh
Preliminary In Vitro Effects of CD8+ T Lymphocyte Specific for the CD20 Alternative Splicing D393-CD20 Peptide Expressed on Burkitt Lymphoma Cells
title Preliminary In Vitro Effects of CD8+ T Lymphocyte Specific for the CD20 Alternative Splicing D393-CD20 Peptide Expressed on Burkitt Lymphoma Cells
title_full Preliminary In Vitro Effects of CD8+ T Lymphocyte Specific for the CD20 Alternative Splicing D393-CD20 Peptide Expressed on Burkitt Lymphoma Cells
title_fullStr Preliminary In Vitro Effects of CD8+ T Lymphocyte Specific for the CD20 Alternative Splicing D393-CD20 Peptide Expressed on Burkitt Lymphoma Cells
title_full_unstemmed Preliminary In Vitro Effects of CD8+ T Lymphocyte Specific for the CD20 Alternative Splicing D393-CD20 Peptide Expressed on Burkitt Lymphoma Cells
title_short Preliminary In Vitro Effects of CD8+ T Lymphocyte Specific for the CD20 Alternative Splicing D393-CD20 Peptide Expressed on Burkitt Lymphoma Cells
title_sort preliminary in vitro effects of cd8+ t lymphocyte specific for the cd20 alternative splicing d393-cd20 peptide expressed on burkitt lymphoma cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852797/
https://www.ncbi.nlm.nih.gov/pubmed/31450932
http://dx.doi.org/10.31557/APJCP.2019.20.8.2563
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