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Investigating the TLR9 mRNA Expression Level in Different Histological Types of Colorectal Polyps
Toll-like receptor 9 (TLR9) is a cellular DNA receptor of the innate immune system which plays a pivotal role in inflammatory response. Recently, changing expression levels of TLR9 has been observed in a wide range of cancer cells; however, there is little information about colorectal polyps. Herein...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
West Asia Organization for Cancer Prevention
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852833/ https://www.ncbi.nlm.nih.gov/pubmed/31450898 http://dx.doi.org/10.31557/APJCP.2019.20.8.2299 |
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author | Rezasoltani, Sama Khatibi, Shirin Pezeshkiyan, Zahra Nazemalhosseini-Mojarad, Ehsan Sharafkhah, Maryam Sadeghi, Amir Asadzadeh Aghdaei, Hamid Zali, Mohammad Reza |
author_facet | Rezasoltani, Sama Khatibi, Shirin Pezeshkiyan, Zahra Nazemalhosseini-Mojarad, Ehsan Sharafkhah, Maryam Sadeghi, Amir Asadzadeh Aghdaei, Hamid Zali, Mohammad Reza |
author_sort | Rezasoltani, Sama |
collection | PubMed |
description | Toll-like receptor 9 (TLR9) is a cellular DNA receptor of the innate immune system which plays a pivotal role in inflammatory response. Recently, changing expression levels of TLR9 has been observed in a wide range of cancer cells; however, there is little information about colorectal polyps. Herein, we assessed the mRNA expression of TLR9 in different colorectal polyp types compared to normal group in order to investigate its expression level during CRC initiation. Fifty-four biopsy samples from colorectal polyp patients and from 20 healthy subjects were collected. The mucosal mRNA expression level of TLR9 gene was identified by real time PCR. Fold change of gene expression was evaluated by 2(-ΔΔct) method. There was a significant relationship between the lower expression of TLR9 gene in the polyp cases compared to normal individuals (P value = 0.0005), Also, decreased TLR9 mRNA expression was obtained in adenomas in contrast to hyperplastic and normal groups (P value = 0.0008). Based on the current results, we hypothesized that aberrant surface expression of TLR9 on tumor cells may promote the growth and invasion of colorectal polyps. Further, TLR9 modulation may have an important impact on the development of novel therapeutic strategies. |
format | Online Article Text |
id | pubmed-6852833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | West Asia Organization for Cancer Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-68528332019-12-12 Investigating the TLR9 mRNA Expression Level in Different Histological Types of Colorectal Polyps Rezasoltani, Sama Khatibi, Shirin Pezeshkiyan, Zahra Nazemalhosseini-Mojarad, Ehsan Sharafkhah, Maryam Sadeghi, Amir Asadzadeh Aghdaei, Hamid Zali, Mohammad Reza Asian Pac J Cancer Prev Research Article Toll-like receptor 9 (TLR9) is a cellular DNA receptor of the innate immune system which plays a pivotal role in inflammatory response. Recently, changing expression levels of TLR9 has been observed in a wide range of cancer cells; however, there is little information about colorectal polyps. Herein, we assessed the mRNA expression of TLR9 in different colorectal polyp types compared to normal group in order to investigate its expression level during CRC initiation. Fifty-four biopsy samples from colorectal polyp patients and from 20 healthy subjects were collected. The mucosal mRNA expression level of TLR9 gene was identified by real time PCR. Fold change of gene expression was evaluated by 2(-ΔΔct) method. There was a significant relationship between the lower expression of TLR9 gene in the polyp cases compared to normal individuals (P value = 0.0005), Also, decreased TLR9 mRNA expression was obtained in adenomas in contrast to hyperplastic and normal groups (P value = 0.0008). Based on the current results, we hypothesized that aberrant surface expression of TLR9 on tumor cells may promote the growth and invasion of colorectal polyps. Further, TLR9 modulation may have an important impact on the development of novel therapeutic strategies. West Asia Organization for Cancer Prevention 2019 /pmc/articles/PMC6852833/ /pubmed/31450898 http://dx.doi.org/10.31557/APJCP.2019.20.8.2299 Text en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) |
spellingShingle | Research Article Rezasoltani, Sama Khatibi, Shirin Pezeshkiyan, Zahra Nazemalhosseini-Mojarad, Ehsan Sharafkhah, Maryam Sadeghi, Amir Asadzadeh Aghdaei, Hamid Zali, Mohammad Reza Investigating the TLR9 mRNA Expression Level in Different Histological Types of Colorectal Polyps |
title | Investigating the TLR9 mRNA Expression Level in Different Histological Types of Colorectal Polyps |
title_full | Investigating the TLR9 mRNA Expression Level in Different Histological Types of Colorectal Polyps |
title_fullStr | Investigating the TLR9 mRNA Expression Level in Different Histological Types of Colorectal Polyps |
title_full_unstemmed | Investigating the TLR9 mRNA Expression Level in Different Histological Types of Colorectal Polyps |
title_short | Investigating the TLR9 mRNA Expression Level in Different Histological Types of Colorectal Polyps |
title_sort | investigating the tlr9 mrna expression level in different histological types of colorectal polyps |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852833/ https://www.ncbi.nlm.nih.gov/pubmed/31450898 http://dx.doi.org/10.31557/APJCP.2019.20.8.2299 |
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