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In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network

The purpose of the present work is to underline the importance of obtaining a standardized procedure to ensure and evaluate both clinical and research usability of human tissue samples. The study, which was carried out by the Biospecimen Science Working Group of the Spanish Biobank Network, is based...

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Autores principales: Esteva-Socias, Margalida, Artiga, María-Jesús, Bahamonde, Olga, Belar, Oihana, Bermudo, Raquel, Castro, Erika, Escámez, Teresa, Fraga, Máximo, Jauregui-Mosquera, Laura, Novoa, Isabel, Peiró-Chova, Lorena, Rejón, Juan-David, Ruiz-Miró, María, Vieiro-Balo, Paula, Villar-Campo, Virginia, Zazo, Sandra, Rábano, Alberto, Villena, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852937/
https://www.ncbi.nlm.nih.gov/pubmed/31718661
http://dx.doi.org/10.1186/s12967-019-2124-8
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author Esteva-Socias, Margalida
Artiga, María-Jesús
Bahamonde, Olga
Belar, Oihana
Bermudo, Raquel
Castro, Erika
Escámez, Teresa
Fraga, Máximo
Jauregui-Mosquera, Laura
Novoa, Isabel
Peiró-Chova, Lorena
Rejón, Juan-David
Ruiz-Miró, María
Vieiro-Balo, Paula
Villar-Campo, Virginia
Zazo, Sandra
Rábano, Alberto
Villena, Cristina
author_facet Esteva-Socias, Margalida
Artiga, María-Jesús
Bahamonde, Olga
Belar, Oihana
Bermudo, Raquel
Castro, Erika
Escámez, Teresa
Fraga, Máximo
Jauregui-Mosquera, Laura
Novoa, Isabel
Peiró-Chova, Lorena
Rejón, Juan-David
Ruiz-Miró, María
Vieiro-Balo, Paula
Villar-Campo, Virginia
Zazo, Sandra
Rábano, Alberto
Villena, Cristina
author_sort Esteva-Socias, Margalida
collection PubMed
description The purpose of the present work is to underline the importance of obtaining a standardized procedure to ensure and evaluate both clinical and research usability of human tissue samples. The study, which was carried out by the Biospecimen Science Working Group of the Spanish Biobank Network, is based on a general overview of the current situation about quality assurance in human tissue biospecimens. It was conducted an exhaustive review of the analytical techniques used to evaluate the quality of human tissue samples over the past 30 years, as well as their reference values if they were published, and classified them according to the biomolecules evaluated: (i) DNA, (ii) RNA, and (iii) soluble or/and fixed proteins for immunochemistry. More than 130 publications released between 1989 and 2019 were analysed, most of them reporting results focused on the analysis of tumour and biopsy samples. A quality assessment proposal with an algorithm has been developed for both frozen tissue samples and formalin-fixed paraffin-embedded (FFPE) samples, according to the expected quality of sample based on the available pre-analytical information and the experience of the participants in the Working Group. The high heterogeneity of human tissue samples and the wide number of pre-analytic factors associated to quality of samples makes it very difficult to harmonize the quality criteria. However, the proposed method to assess human tissue sample integrity and antigenicity will not only help to evaluate whether stored human tissue samples fit for the purpose of biomarker development, but will also allow to perform further studies, such as assessing the impact of different pre-analytical factors on very well characterized samples or evaluating the readjustment of tissue sample collection, processing and storing procedures. By ensuring the quality of the samples used on research, the reproducibility of scientific results will be guaranteed.
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spelling pubmed-68529372019-11-20 In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network Esteva-Socias, Margalida Artiga, María-Jesús Bahamonde, Olga Belar, Oihana Bermudo, Raquel Castro, Erika Escámez, Teresa Fraga, Máximo Jauregui-Mosquera, Laura Novoa, Isabel Peiró-Chova, Lorena Rejón, Juan-David Ruiz-Miró, María Vieiro-Balo, Paula Villar-Campo, Virginia Zazo, Sandra Rábano, Alberto Villena, Cristina J Transl Med Review The purpose of the present work is to underline the importance of obtaining a standardized procedure to ensure and evaluate both clinical and research usability of human tissue samples. The study, which was carried out by the Biospecimen Science Working Group of the Spanish Biobank Network, is based on a general overview of the current situation about quality assurance in human tissue biospecimens. It was conducted an exhaustive review of the analytical techniques used to evaluate the quality of human tissue samples over the past 30 years, as well as their reference values if they were published, and classified them according to the biomolecules evaluated: (i) DNA, (ii) RNA, and (iii) soluble or/and fixed proteins for immunochemistry. More than 130 publications released between 1989 and 2019 were analysed, most of them reporting results focused on the analysis of tumour and biopsy samples. A quality assessment proposal with an algorithm has been developed for both frozen tissue samples and formalin-fixed paraffin-embedded (FFPE) samples, according to the expected quality of sample based on the available pre-analytical information and the experience of the participants in the Working Group. The high heterogeneity of human tissue samples and the wide number of pre-analytic factors associated to quality of samples makes it very difficult to harmonize the quality criteria. However, the proposed method to assess human tissue sample integrity and antigenicity will not only help to evaluate whether stored human tissue samples fit for the purpose of biomarker development, but will also allow to perform further studies, such as assessing the impact of different pre-analytical factors on very well characterized samples or evaluating the readjustment of tissue sample collection, processing and storing procedures. By ensuring the quality of the samples used on research, the reproducibility of scientific results will be guaranteed. BioMed Central 2019-11-12 /pmc/articles/PMC6852937/ /pubmed/31718661 http://dx.doi.org/10.1186/s12967-019-2124-8 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Esteva-Socias, Margalida
Artiga, María-Jesús
Bahamonde, Olga
Belar, Oihana
Bermudo, Raquel
Castro, Erika
Escámez, Teresa
Fraga, Máximo
Jauregui-Mosquera, Laura
Novoa, Isabel
Peiró-Chova, Lorena
Rejón, Juan-David
Ruiz-Miró, María
Vieiro-Balo, Paula
Villar-Campo, Virginia
Zazo, Sandra
Rábano, Alberto
Villena, Cristina
In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network
title In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network
title_full In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network
title_fullStr In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network
title_full_unstemmed In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network
title_short In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network
title_sort in search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue biospecimen research working group of the spanish biobank network
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852937/
https://www.ncbi.nlm.nih.gov/pubmed/31718661
http://dx.doi.org/10.1186/s12967-019-2124-8
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