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Identification of loci associated with conception rate in primiparous Holstein cows

BACKGROUND: Subfertility is a major issue facing the dairy industry as the average US Holstein cow conception rate (CCR) is approximately 35%. The genetics underlying the physiological processes responsible for CCR, the proportion of cows able to conceive and maintain a pregnancy at each breeding, a...

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Autores principales: Kiser, Jennifer N., Clancey, Erin, Moraes, Joao G. N., Dalton, Joseph, Burns, Gregory W., Spencer, Thomas E., Neibergs, Holly L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852976/
https://www.ncbi.nlm.nih.gov/pubmed/31718557
http://dx.doi.org/10.1186/s12864-019-6203-2
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author Kiser, Jennifer N.
Clancey, Erin
Moraes, Joao G. N.
Dalton, Joseph
Burns, Gregory W.
Spencer, Thomas E.
Neibergs, Holly L.
author_facet Kiser, Jennifer N.
Clancey, Erin
Moraes, Joao G. N.
Dalton, Joseph
Burns, Gregory W.
Spencer, Thomas E.
Neibergs, Holly L.
author_sort Kiser, Jennifer N.
collection PubMed
description BACKGROUND: Subfertility is a major issue facing the dairy industry as the average US Holstein cow conception rate (CCR) is approximately 35%. The genetics underlying the physiological processes responsible for CCR, the proportion of cows able to conceive and maintain a pregnancy at each breeding, are not well characterized. The objectives of this study were to identify loci, positional candidate genes, and transcription factor binding sites (TFBS) associated with CCR and determine if there was a genetic correlation between CCR and milk production in primiparous Holstein cows. Cows were bred via artificial insemination (AI) at either observed estrus or timed AI and pregnancy status was determined at day 35 post-insemination. Additive, dominant, and recessive efficient mixed model association expedited (EMMAX) models were used in two genome-wide association analyses (GWAA). One GWAA focused on CCR at first service (CCR1) comparing cows that conceived and maintained pregnancy to day 35 after the first AI (n = 494) to those that were open after the first AI (n = 538). The second GWAA investigated loci associated with the number of times bred (TBRD) required for conception in cows that either conceived after the first AI (n = 494) or repeated services (n = 472). RESULTS: The CCR1 GWAA identified 123, 198, and 76 loci associated (P < 5 × 10(− 08)) in additive, dominant, and recessive models, respectively. The TBRD GWAA identified 66, 95, and 33 loci associated (P < 5 × 10(− 08)) in additive, dominant, and recessive models, respectively. Four of the top five loci were shared in CCR1 and TBRD for each GWAA model. Many of the associated loci harbored positional candidate genes and TFBS with putative functional relevance to fertility. Thirty-six of the loci were validated in previous GWAA studies across multiple breeds. None of the CCR1 or TBRD associated loci were associated with milk production, nor was their significance with phenotypic and genetic correlations to 305-day milk production. CONCLUSIONS: The identification and validation of loci, positional candidate genes, and TFBS associated with CCR1 and TBRD can be utilized to improve, and further characterize the processes involved in cattle fertility.
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spelling pubmed-68529762019-11-21 Identification of loci associated with conception rate in primiparous Holstein cows Kiser, Jennifer N. Clancey, Erin Moraes, Joao G. N. Dalton, Joseph Burns, Gregory W. Spencer, Thomas E. Neibergs, Holly L. BMC Genomics Research Article BACKGROUND: Subfertility is a major issue facing the dairy industry as the average US Holstein cow conception rate (CCR) is approximately 35%. The genetics underlying the physiological processes responsible for CCR, the proportion of cows able to conceive and maintain a pregnancy at each breeding, are not well characterized. The objectives of this study were to identify loci, positional candidate genes, and transcription factor binding sites (TFBS) associated with CCR and determine if there was a genetic correlation between CCR and milk production in primiparous Holstein cows. Cows were bred via artificial insemination (AI) at either observed estrus or timed AI and pregnancy status was determined at day 35 post-insemination. Additive, dominant, and recessive efficient mixed model association expedited (EMMAX) models were used in two genome-wide association analyses (GWAA). One GWAA focused on CCR at first service (CCR1) comparing cows that conceived and maintained pregnancy to day 35 after the first AI (n = 494) to those that were open after the first AI (n = 538). The second GWAA investigated loci associated with the number of times bred (TBRD) required for conception in cows that either conceived after the first AI (n = 494) or repeated services (n = 472). RESULTS: The CCR1 GWAA identified 123, 198, and 76 loci associated (P < 5 × 10(− 08)) in additive, dominant, and recessive models, respectively. The TBRD GWAA identified 66, 95, and 33 loci associated (P < 5 × 10(− 08)) in additive, dominant, and recessive models, respectively. Four of the top five loci were shared in CCR1 and TBRD for each GWAA model. Many of the associated loci harbored positional candidate genes and TFBS with putative functional relevance to fertility. Thirty-six of the loci were validated in previous GWAA studies across multiple breeds. None of the CCR1 or TBRD associated loci were associated with milk production, nor was their significance with phenotypic and genetic correlations to 305-day milk production. CONCLUSIONS: The identification and validation of loci, positional candidate genes, and TFBS associated with CCR1 and TBRD can be utilized to improve, and further characterize the processes involved in cattle fertility. BioMed Central 2019-11-12 /pmc/articles/PMC6852976/ /pubmed/31718557 http://dx.doi.org/10.1186/s12864-019-6203-2 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Kiser, Jennifer N.
Clancey, Erin
Moraes, Joao G. N.
Dalton, Joseph
Burns, Gregory W.
Spencer, Thomas E.
Neibergs, Holly L.
Identification of loci associated with conception rate in primiparous Holstein cows
title Identification of loci associated with conception rate in primiparous Holstein cows
title_full Identification of loci associated with conception rate in primiparous Holstein cows
title_fullStr Identification of loci associated with conception rate in primiparous Holstein cows
title_full_unstemmed Identification of loci associated with conception rate in primiparous Holstein cows
title_short Identification of loci associated with conception rate in primiparous Holstein cows
title_sort identification of loci associated with conception rate in primiparous holstein cows
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852976/
https://www.ncbi.nlm.nih.gov/pubmed/31718557
http://dx.doi.org/10.1186/s12864-019-6203-2
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