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Comparison of the inoculum size effects of antibiotics on IMP-6 β-lactamase-producing Enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes

Almost all cases of carbapenemase-producing Enterobacteriaceae infections in Japan are caused by bla(IMP)-positive Enterobacteriaceae (especially bla(IMP-6)) and infections caused by other types of carbapenemase-producing Enterobacteriaceae are quite rare. We examined drug resistance genes co-harbor...

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Autores principales: Ogawa, Yoshihiko, Nakano, Ryuichi, Kasahara, Kei, Mizuno, Tomoki, Hirai, Nobuyasu, Nakano, Akiyo, Suzuki, Yuki, Kakuta, Naoki, Masui, Takashi, Yano, Hisakazu, Mikasa, Keiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6853314/
https://www.ncbi.nlm.nih.gov/pubmed/31721789
http://dx.doi.org/10.1371/journal.pone.0225210
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author Ogawa, Yoshihiko
Nakano, Ryuichi
Kasahara, Kei
Mizuno, Tomoki
Hirai, Nobuyasu
Nakano, Akiyo
Suzuki, Yuki
Kakuta, Naoki
Masui, Takashi
Yano, Hisakazu
Mikasa, Keiichi
author_facet Ogawa, Yoshihiko
Nakano, Ryuichi
Kasahara, Kei
Mizuno, Tomoki
Hirai, Nobuyasu
Nakano, Akiyo
Suzuki, Yuki
Kakuta, Naoki
Masui, Takashi
Yano, Hisakazu
Mikasa, Keiichi
author_sort Ogawa, Yoshihiko
collection PubMed
description Almost all cases of carbapenemase-producing Enterobacteriaceae infections in Japan are caused by bla(IMP)-positive Enterobacteriaceae (especially bla(IMP-6)) and infections caused by other types of carbapenemase-producing Enterobacteriaceae are quite rare. We examined drug resistance genes co-harboring with bla(IMP-6) and their inoculum size effects. We screened β-lactamase genes, plasmid-mediated quinolone resistance (PMQR) genes, and aminoglycoside-modifying enzyme genes by PCR and performed sequencing for 14 bla(IMP-6)-positive Enterobacteriaceae. Further, all PMQR-positive isolates were submitted to conjugation and inoculum effect evaluation. Our data showed that 13 of the 14 isolates harbored CTX-M-2 and one co-harbored CTX-M-2 and CTX-M-1 as extended-spectrum β-lactamases. All isolates carried one or more PMQRs; aac(6’)-Ib-cr was the most prevalent (92.8%), and was followed by oqxA (64.3%), qnrS (50%), oqxAB (21.4%), and qnrB (14.3%). However, Klebsiella pneumoniae contains chromosomal OqxAB. Inoculum size effects were significant in all strains for meropenem, 13 strains for imipenem, 7 for levofloxacin, and 3 for amikacin. We observed that 11 of the experimental strains (100%), 8 strains (72.7%), and 1 strain showed inoculum size effects for meropenem, imipenem, and amikacin, respectively. However, four strains harbored qnr genes and two strains harbored qnr genes and QRDR mutations concurrently; no inoculum size effect was seen for levofloxacin. The bla(IMP-6)-positive Enterobacteriaceae that we studied was found to harbor at least one plasmid-mediated drug resistance gene. The inoculum size effect for carbapenems was thought to be mainly due to IMP-6-type metallo-β-lactamase; however qnrB and qnrS also had a minimal impact on the inoculum size effect for levofloxacin.
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spelling pubmed-68533142019-11-22 Comparison of the inoculum size effects of antibiotics on IMP-6 β-lactamase-producing Enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes Ogawa, Yoshihiko Nakano, Ryuichi Kasahara, Kei Mizuno, Tomoki Hirai, Nobuyasu Nakano, Akiyo Suzuki, Yuki Kakuta, Naoki Masui, Takashi Yano, Hisakazu Mikasa, Keiichi PLoS One Research Article Almost all cases of carbapenemase-producing Enterobacteriaceae infections in Japan are caused by bla(IMP)-positive Enterobacteriaceae (especially bla(IMP-6)) and infections caused by other types of carbapenemase-producing Enterobacteriaceae are quite rare. We examined drug resistance genes co-harboring with bla(IMP-6) and their inoculum size effects. We screened β-lactamase genes, plasmid-mediated quinolone resistance (PMQR) genes, and aminoglycoside-modifying enzyme genes by PCR and performed sequencing for 14 bla(IMP-6)-positive Enterobacteriaceae. Further, all PMQR-positive isolates were submitted to conjugation and inoculum effect evaluation. Our data showed that 13 of the 14 isolates harbored CTX-M-2 and one co-harbored CTX-M-2 and CTX-M-1 as extended-spectrum β-lactamases. All isolates carried one or more PMQRs; aac(6’)-Ib-cr was the most prevalent (92.8%), and was followed by oqxA (64.3%), qnrS (50%), oqxAB (21.4%), and qnrB (14.3%). However, Klebsiella pneumoniae contains chromosomal OqxAB. Inoculum size effects were significant in all strains for meropenem, 13 strains for imipenem, 7 for levofloxacin, and 3 for amikacin. We observed that 11 of the experimental strains (100%), 8 strains (72.7%), and 1 strain showed inoculum size effects for meropenem, imipenem, and amikacin, respectively. However, four strains harbored qnr genes and two strains harbored qnr genes and QRDR mutations concurrently; no inoculum size effect was seen for levofloxacin. The bla(IMP-6)-positive Enterobacteriaceae that we studied was found to harbor at least one plasmid-mediated drug resistance gene. The inoculum size effect for carbapenems was thought to be mainly due to IMP-6-type metallo-β-lactamase; however qnrB and qnrS also had a minimal impact on the inoculum size effect for levofloxacin. Public Library of Science 2019-11-13 /pmc/articles/PMC6853314/ /pubmed/31721789 http://dx.doi.org/10.1371/journal.pone.0225210 Text en © 2019 Ogawa et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ogawa, Yoshihiko
Nakano, Ryuichi
Kasahara, Kei
Mizuno, Tomoki
Hirai, Nobuyasu
Nakano, Akiyo
Suzuki, Yuki
Kakuta, Naoki
Masui, Takashi
Yano, Hisakazu
Mikasa, Keiichi
Comparison of the inoculum size effects of antibiotics on IMP-6 β-lactamase-producing Enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes
title Comparison of the inoculum size effects of antibiotics on IMP-6 β-lactamase-producing Enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes
title_full Comparison of the inoculum size effects of antibiotics on IMP-6 β-lactamase-producing Enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes
title_fullStr Comparison of the inoculum size effects of antibiotics on IMP-6 β-lactamase-producing Enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes
title_full_unstemmed Comparison of the inoculum size effects of antibiotics on IMP-6 β-lactamase-producing Enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes
title_short Comparison of the inoculum size effects of antibiotics on IMP-6 β-lactamase-producing Enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes
title_sort comparison of the inoculum size effects of antibiotics on imp-6 β-lactamase-producing enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6853314/
https://www.ncbi.nlm.nih.gov/pubmed/31721789
http://dx.doi.org/10.1371/journal.pone.0225210
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