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Temporal stability of fMRI in medetomidine-anesthetized rats

Medetomidine has become a popular choice for anesthetizing rats during long-lasting sessions of blood-oxygen-level dependent (BOLD) functional magnetic resonance imaging (fMRI). Despite this, it has not yet been thoroughly established how commonly reported fMRI readouts evolve over several hours of...

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Autores principales: Sirmpilatze, Nikoloz, Baudewig, Jürgen, Boretius, Susann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6853937/
https://www.ncbi.nlm.nih.gov/pubmed/31723186
http://dx.doi.org/10.1038/s41598-019-53144-y
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author Sirmpilatze, Nikoloz
Baudewig, Jürgen
Boretius, Susann
author_facet Sirmpilatze, Nikoloz
Baudewig, Jürgen
Boretius, Susann
author_sort Sirmpilatze, Nikoloz
collection PubMed
description Medetomidine has become a popular choice for anesthetizing rats during long-lasting sessions of blood-oxygen-level dependent (BOLD) functional magnetic resonance imaging (fMRI). Despite this, it has not yet been thoroughly established how commonly reported fMRI readouts evolve over several hours of medetomidine anesthesia and how they are affected by the precise timing, dose, and route of administration. We used four different protocols of medetomidine administration to anesthetize rats for up to six hours and repeatedly evaluated somatosensory stimulus-evoked BOLD responses and resting state functional connectivity. We found that the temporal evolution of fMRI readouts strongly depended on the method of administration. Intravenous administration of a medetomidine bolus (0.05 mg/kg), combined with a subsequent continuous infusion (0.1 mg/kg/h), led to temporally stable measures of stimulus-evoked activity and functional connectivity throughout the anesthesia. Deviating from the above protocol—by omitting the bolus, lowering the medetomidine dose, or using the subcutaneous route—compromised the stability of these measures in the initial two-hour period. We conclude that both an appropriate protocol of medetomidine administration and a suitable timing of fMRI experiments are crucial for obtaining consistent results. These factors should be considered for the design and interpretation of future rat fMRI studies.
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spelling pubmed-68539372019-11-19 Temporal stability of fMRI in medetomidine-anesthetized rats Sirmpilatze, Nikoloz Baudewig, Jürgen Boretius, Susann Sci Rep Article Medetomidine has become a popular choice for anesthetizing rats during long-lasting sessions of blood-oxygen-level dependent (BOLD) functional magnetic resonance imaging (fMRI). Despite this, it has not yet been thoroughly established how commonly reported fMRI readouts evolve over several hours of medetomidine anesthesia and how they are affected by the precise timing, dose, and route of administration. We used four different protocols of medetomidine administration to anesthetize rats for up to six hours and repeatedly evaluated somatosensory stimulus-evoked BOLD responses and resting state functional connectivity. We found that the temporal evolution of fMRI readouts strongly depended on the method of administration. Intravenous administration of a medetomidine bolus (0.05 mg/kg), combined with a subsequent continuous infusion (0.1 mg/kg/h), led to temporally stable measures of stimulus-evoked activity and functional connectivity throughout the anesthesia. Deviating from the above protocol—by omitting the bolus, lowering the medetomidine dose, or using the subcutaneous route—compromised the stability of these measures in the initial two-hour period. We conclude that both an appropriate protocol of medetomidine administration and a suitable timing of fMRI experiments are crucial for obtaining consistent results. These factors should be considered for the design and interpretation of future rat fMRI studies. Nature Publishing Group UK 2019-11-13 /pmc/articles/PMC6853937/ /pubmed/31723186 http://dx.doi.org/10.1038/s41598-019-53144-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sirmpilatze, Nikoloz
Baudewig, Jürgen
Boretius, Susann
Temporal stability of fMRI in medetomidine-anesthetized rats
title Temporal stability of fMRI in medetomidine-anesthetized rats
title_full Temporal stability of fMRI in medetomidine-anesthetized rats
title_fullStr Temporal stability of fMRI in medetomidine-anesthetized rats
title_full_unstemmed Temporal stability of fMRI in medetomidine-anesthetized rats
title_short Temporal stability of fMRI in medetomidine-anesthetized rats
title_sort temporal stability of fmri in medetomidine-anesthetized rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6853937/
https://www.ncbi.nlm.nih.gov/pubmed/31723186
http://dx.doi.org/10.1038/s41598-019-53144-y
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