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Alternative Sigma Factor B in Bovine Mastitis-Causing Staphylococcus aureus: Characterization of Its Role in Biofilm Formation, Resistance to Hydrogen Peroxide Stress, Regulon Members

This study examines treatments of the bacterial pathogen Staphylococcus aureus, namely, in the context of its being a major cause of subclinical bovine mastitis. Such infections caused by S. aureus among dairy cows are difficult to detect and can easily become chronic, leading to reduced productivit...

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Autores principales: Supa-amornkul, Sirirak, Mongkolsuk, Paninee, Summpunn, Pijug, Chaiyakunvat, Pongkorn, Navaratdusit, Warisara, Jiarpinitnun, Chutima, Chaturongakul, Soraya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6853994/
https://www.ncbi.nlm.nih.gov/pubmed/31787937
http://dx.doi.org/10.3389/fmicb.2019.02493
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author Supa-amornkul, Sirirak
Mongkolsuk, Paninee
Summpunn, Pijug
Chaiyakunvat, Pongkorn
Navaratdusit, Warisara
Jiarpinitnun, Chutima
Chaturongakul, Soraya
author_facet Supa-amornkul, Sirirak
Mongkolsuk, Paninee
Summpunn, Pijug
Chaiyakunvat, Pongkorn
Navaratdusit, Warisara
Jiarpinitnun, Chutima
Chaturongakul, Soraya
author_sort Supa-amornkul, Sirirak
collection PubMed
description This study examines treatments of the bacterial pathogen Staphylococcus aureus, namely, in the context of its being a major cause of subclinical bovine mastitis. Such infections caused by S. aureus among dairy cows are difficult to detect and can easily become chronic, leading to reduced productivity and large losses for dairy manufacturers. In this study, the role of alternative sigma factor B (σ(B)), which has been shown to be a global regulator for S. aureus infections, was explored in a mastitis-causing S. aureus strain, RF122. For comparison with the wild-type strain, a sigB null (ΔsigB) mutant was constructed and analyzed for its phenotypes and transcriptome. Our study found that σ(B) is essential for biofilm formation as the ΔsigB mutant strain produced significantly less biofilm than did the wild-type strain at 48 h. σ(B) is involved in response to H(2)O(2) stress. However, σ(B) plays a minor or no role in resistance to antiseptics (e.g., povidone-iodine and chlorhexidine), resistance to tested antibiotics, hemolysin activity, and invasion ability. RNA sequencing identified 225 σ(B)-dependent genes, of which 171 are positively regulated and 54 are negatively regulated. The identified genes are involved in stress response, pathogenesis, and metabolic mechanisms. Quantitative TaqMan RT-PCR was performed to verify the RNA sequencing results; i.e., σ(B) is a positive regulator for asp23, sarA, katA, yabJ, sodA, SAB2006c, and nrdD expressions. In the RF122 strain, σ(B) plays a role in biofilm formation, general stress response (e.g., H(2)O(2)), and regulation of virulence factors and virulence-associated genes.
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spelling pubmed-68539942019-11-29 Alternative Sigma Factor B in Bovine Mastitis-Causing Staphylococcus aureus: Characterization of Its Role in Biofilm Formation, Resistance to Hydrogen Peroxide Stress, Regulon Members Supa-amornkul, Sirirak Mongkolsuk, Paninee Summpunn, Pijug Chaiyakunvat, Pongkorn Navaratdusit, Warisara Jiarpinitnun, Chutima Chaturongakul, Soraya Front Microbiol Microbiology This study examines treatments of the bacterial pathogen Staphylococcus aureus, namely, in the context of its being a major cause of subclinical bovine mastitis. Such infections caused by S. aureus among dairy cows are difficult to detect and can easily become chronic, leading to reduced productivity and large losses for dairy manufacturers. In this study, the role of alternative sigma factor B (σ(B)), which has been shown to be a global regulator for S. aureus infections, was explored in a mastitis-causing S. aureus strain, RF122. For comparison with the wild-type strain, a sigB null (ΔsigB) mutant was constructed and analyzed for its phenotypes and transcriptome. Our study found that σ(B) is essential for biofilm formation as the ΔsigB mutant strain produced significantly less biofilm than did the wild-type strain at 48 h. σ(B) is involved in response to H(2)O(2) stress. However, σ(B) plays a minor or no role in resistance to antiseptics (e.g., povidone-iodine and chlorhexidine), resistance to tested antibiotics, hemolysin activity, and invasion ability. RNA sequencing identified 225 σ(B)-dependent genes, of which 171 are positively regulated and 54 are negatively regulated. The identified genes are involved in stress response, pathogenesis, and metabolic mechanisms. Quantitative TaqMan RT-PCR was performed to verify the RNA sequencing results; i.e., σ(B) is a positive regulator for asp23, sarA, katA, yabJ, sodA, SAB2006c, and nrdD expressions. In the RF122 strain, σ(B) plays a role in biofilm formation, general stress response (e.g., H(2)O(2)), and regulation of virulence factors and virulence-associated genes. Frontiers Media S.A. 2019-11-07 /pmc/articles/PMC6853994/ /pubmed/31787937 http://dx.doi.org/10.3389/fmicb.2019.02493 Text en Copyright © 2019 Supa-amornkul, Mongkolsuk, Summpunn, Chaiyakunvat, Navaratdusit, Jiarpinitnun and Chaturongakul. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Supa-amornkul, Sirirak
Mongkolsuk, Paninee
Summpunn, Pijug
Chaiyakunvat, Pongkorn
Navaratdusit, Warisara
Jiarpinitnun, Chutima
Chaturongakul, Soraya
Alternative Sigma Factor B in Bovine Mastitis-Causing Staphylococcus aureus: Characterization of Its Role in Biofilm Formation, Resistance to Hydrogen Peroxide Stress, Regulon Members
title Alternative Sigma Factor B in Bovine Mastitis-Causing Staphylococcus aureus: Characterization of Its Role in Biofilm Formation, Resistance to Hydrogen Peroxide Stress, Regulon Members
title_full Alternative Sigma Factor B in Bovine Mastitis-Causing Staphylococcus aureus: Characterization of Its Role in Biofilm Formation, Resistance to Hydrogen Peroxide Stress, Regulon Members
title_fullStr Alternative Sigma Factor B in Bovine Mastitis-Causing Staphylococcus aureus: Characterization of Its Role in Biofilm Formation, Resistance to Hydrogen Peroxide Stress, Regulon Members
title_full_unstemmed Alternative Sigma Factor B in Bovine Mastitis-Causing Staphylococcus aureus: Characterization of Its Role in Biofilm Formation, Resistance to Hydrogen Peroxide Stress, Regulon Members
title_short Alternative Sigma Factor B in Bovine Mastitis-Causing Staphylococcus aureus: Characterization of Its Role in Biofilm Formation, Resistance to Hydrogen Peroxide Stress, Regulon Members
title_sort alternative sigma factor b in bovine mastitis-causing staphylococcus aureus: characterization of its role in biofilm formation, resistance to hydrogen peroxide stress, regulon members
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6853994/
https://www.ncbi.nlm.nih.gov/pubmed/31787937
http://dx.doi.org/10.3389/fmicb.2019.02493
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