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Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii

While the role of TOR kinase in the chloroplast biogenesis and transcriptional regulation of photosynthesis is well documented in Arabidopsis, the functional relevance of this metabolic sensor kinase in chloroplast–mitochondria cross talk is unknown. Using Chlamydomonas reinhardtii as the model syst...

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Autores principales: Upadhyaya, Shivani, Rao, Basuthkar Jagadeeshwar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854518/
https://www.ncbi.nlm.nih.gov/pubmed/31832599
http://dx.doi.org/10.1002/pld3.184
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author Upadhyaya, Shivani
Rao, Basuthkar Jagadeeshwar
author_facet Upadhyaya, Shivani
Rao, Basuthkar Jagadeeshwar
author_sort Upadhyaya, Shivani
collection PubMed
description While the role of TOR kinase in the chloroplast biogenesis and transcriptional regulation of photosynthesis is well documented in Arabidopsis, the functional relevance of this metabolic sensor kinase in chloroplast–mitochondria cross talk is unknown. Using Chlamydomonas reinhardtii as the model system, we demonstrate the role of TOR kinase in the regulation of chloroplast and mitochondrial functions: We show that TOR kinase inhibition impairs the maintenance of high ETR associated with PSII and low NPQ and inhibits efficient state transitions between PSII and PSI. While compromised photosynthetic functions are observed in TOR kinase inhibited cells, same conditions lead to augmentation in mitochondrial basal respiration rate by twofold and concomitantly a rise in ATP production. Interestingly, such upregulated mitochondrial functions in TOR‐inhibited cells are mediated by fragmented mitochondria via upregulating COXIIb and downregulating Hxk1 and AOX1 protein levels. We propose that TOR kinase may act as a sensor that counter‐regulates chloroplast versus mitochondrial functions in a normal C. reinhardtii cell.
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spelling pubmed-68545182019-12-12 Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii Upadhyaya, Shivani Rao, Basuthkar Jagadeeshwar Plant Direct Original Research While the role of TOR kinase in the chloroplast biogenesis and transcriptional regulation of photosynthesis is well documented in Arabidopsis, the functional relevance of this metabolic sensor kinase in chloroplast–mitochondria cross talk is unknown. Using Chlamydomonas reinhardtii as the model system, we demonstrate the role of TOR kinase in the regulation of chloroplast and mitochondrial functions: We show that TOR kinase inhibition impairs the maintenance of high ETR associated with PSII and low NPQ and inhibits efficient state transitions between PSII and PSI. While compromised photosynthetic functions are observed in TOR kinase inhibited cells, same conditions lead to augmentation in mitochondrial basal respiration rate by twofold and concomitantly a rise in ATP production. Interestingly, such upregulated mitochondrial functions in TOR‐inhibited cells are mediated by fragmented mitochondria via upregulating COXIIb and downregulating Hxk1 and AOX1 protein levels. We propose that TOR kinase may act as a sensor that counter‐regulates chloroplast versus mitochondrial functions in a normal C. reinhardtii cell. John Wiley and Sons Inc. 2019-11-14 /pmc/articles/PMC6854518/ /pubmed/31832599 http://dx.doi.org/10.1002/pld3.184 Text en © 2019 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Research
Upadhyaya, Shivani
Rao, Basuthkar Jagadeeshwar
Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii
title Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii
title_full Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii
title_fullStr Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii
title_full_unstemmed Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii
title_short Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii
title_sort reciprocal regulation of photosynthesis and mitochondrial respiration by tor kinase in chlamydomonas reinhardtii
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854518/
https://www.ncbi.nlm.nih.gov/pubmed/31832599
http://dx.doi.org/10.1002/pld3.184
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