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Infiltration and In-Tissue Polymerization of Photocross-Linked Hydrogel for Effective Fixation of Implants into Cartilage—An In Vitro Study

[Image: see text] Effective and biocompatible fixation of implants into cartilage defects has yet to be successfully achieved. [Poly-d,l-lactic acid/polyethyleneglycol/poly-d,l-lactic acid] (PDLLA-PEG) is a chondrosupportive scaffold that is photocross-linked using the visible-light photoinitiator l...

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Autores principales: Kuang, Biao, Yang, Yuanheng, Lin, Hang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854571/
https://www.ncbi.nlm.nih.gov/pubmed/31737812
http://dx.doi.org/10.1021/acsomega.9b02270
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author Kuang, Biao
Yang, Yuanheng
Lin, Hang
author_facet Kuang, Biao
Yang, Yuanheng
Lin, Hang
author_sort Kuang, Biao
collection PubMed
description [Image: see text] Effective and biocompatible fixation of implants into cartilage defects has yet to be successfully achieved. [Poly-d,l-lactic acid/polyethyleneglycol/poly-d,l-lactic acid] (PDLLA-PEG) is a chondrosupportive scaffold that is photocross-linked using the visible-light photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP). Interestingly, LAP and its monomer DLLA-EG are able to infiltrate the cartilage and form hydrogels upon the detection of light. After the infiltration of LAP and DLLA-EG into the implant and host cartilage, an interconnected and continuous hydrogel structure is formed which fixes the implant within the host cartilage. A mechanical test shows that the infiltrated group displays a significantly higher push-out force than the group that has not been infiltrated (the traditional fibrin fixation group). Surprisingly, the in-cartilage hydrogel also reduces the release of sulfated glycosaminoglycan from cartilage explants. However, infiltration does not affect the cell viability or the expression of cartilage marker genes. This new strategy thus represents a biocompatible and efficient method to fix implants into host tissues.
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spelling pubmed-68545712019-11-15 Infiltration and In-Tissue Polymerization of Photocross-Linked Hydrogel for Effective Fixation of Implants into Cartilage—An In Vitro Study Kuang, Biao Yang, Yuanheng Lin, Hang ACS Omega [Image: see text] Effective and biocompatible fixation of implants into cartilage defects has yet to be successfully achieved. [Poly-d,l-lactic acid/polyethyleneglycol/poly-d,l-lactic acid] (PDLLA-PEG) is a chondrosupportive scaffold that is photocross-linked using the visible-light photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP). Interestingly, LAP and its monomer DLLA-EG are able to infiltrate the cartilage and form hydrogels upon the detection of light. After the infiltration of LAP and DLLA-EG into the implant and host cartilage, an interconnected and continuous hydrogel structure is formed which fixes the implant within the host cartilage. A mechanical test shows that the infiltrated group displays a significantly higher push-out force than the group that has not been infiltrated (the traditional fibrin fixation group). Surprisingly, the in-cartilage hydrogel also reduces the release of sulfated glycosaminoglycan from cartilage explants. However, infiltration does not affect the cell viability or the expression of cartilage marker genes. This new strategy thus represents a biocompatible and efficient method to fix implants into host tissues. American Chemical Society 2019-10-28 /pmc/articles/PMC6854571/ /pubmed/31737812 http://dx.doi.org/10.1021/acsomega.9b02270 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Kuang, Biao
Yang, Yuanheng
Lin, Hang
Infiltration and In-Tissue Polymerization of Photocross-Linked Hydrogel for Effective Fixation of Implants into Cartilage—An In Vitro Study
title Infiltration and In-Tissue Polymerization of Photocross-Linked Hydrogel for Effective Fixation of Implants into Cartilage—An In Vitro Study
title_full Infiltration and In-Tissue Polymerization of Photocross-Linked Hydrogel for Effective Fixation of Implants into Cartilage—An In Vitro Study
title_fullStr Infiltration and In-Tissue Polymerization of Photocross-Linked Hydrogel for Effective Fixation of Implants into Cartilage—An In Vitro Study
title_full_unstemmed Infiltration and In-Tissue Polymerization of Photocross-Linked Hydrogel for Effective Fixation of Implants into Cartilage—An In Vitro Study
title_short Infiltration and In-Tissue Polymerization of Photocross-Linked Hydrogel for Effective Fixation of Implants into Cartilage—An In Vitro Study
title_sort infiltration and in-tissue polymerization of photocross-linked hydrogel for effective fixation of implants into cartilage—an in vitro study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854571/
https://www.ncbi.nlm.nih.gov/pubmed/31737812
http://dx.doi.org/10.1021/acsomega.9b02270
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AT yangyuanheng infiltrationandintissuepolymerizationofphotocrosslinkedhydrogelforeffectivefixationofimplantsintocartilageaninvitrostudy
AT linhang infiltrationandintissuepolymerizationofphotocrosslinkedhydrogelforeffectivefixationofimplantsintocartilageaninvitrostudy