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Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants

Although Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and som...

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Autores principales: Okura, Masatoshi, Maruyama, Fumito, Ota, Atsushi, Tanaka, Takeshi, Matoba, Yohei, Osawa, Aya, Sadaat, Sayed Mushtaq, Osaki, Makoto, Toyoda, Atsushi, Ogura, Yoshitoshi, Hayashi, Tetsuya, Takamatsu, Daisuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854688/
https://www.ncbi.nlm.nih.gov/pubmed/31727180
http://dx.doi.org/10.1186/s13567-019-0708-1
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author Okura, Masatoshi
Maruyama, Fumito
Ota, Atsushi
Tanaka, Takeshi
Matoba, Yohei
Osawa, Aya
Sadaat, Sayed Mushtaq
Osaki, Makoto
Toyoda, Atsushi
Ogura, Yoshitoshi
Hayashi, Tetsuya
Takamatsu, Daisuke
author_facet Okura, Masatoshi
Maruyama, Fumito
Ota, Atsushi
Tanaka, Takeshi
Matoba, Yohei
Osawa, Aya
Sadaat, Sayed Mushtaq
Osaki, Makoto
Toyoda, Atsushi
Ogura, Yoshitoshi
Hayashi, Tetsuya
Takamatsu, Daisuke
author_sort Okura, Masatoshi
collection PubMed
description Although Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and some of these isolates were reclassified as the novel species Streptococcus ruminantium. In Japan, biochemically identified S. suis is frequently isolated from diseased ruminants; however, such isolates have not yet been identified accurately, and their aetiological importance in ruminants is unclear. Therefore, to understand the importance of S. suis and S. suis-like bacteria in ruminants, we reclassified S. suis isolates from ruminants according to the updated classification and investigated their genetic diversity. Although both S. suis and S. ruminantium were isolated from healthy and diseased ruminants, most of the isolates from diseased animals were S. ruminantium, implying that S. ruminantium is more likely to be associated with ruminant disease than S. suis. However, the ruminant S. suis and S. ruminantium isolates from diseased animals were classified into diverse genotypes rather than belonging to certain clonal groups. Genome sequence analysis of 20 S. ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. We further developed an S. ruminantium-specific PCR assay to aid in the identification of this bacterium. The information obtained and the method established in this study will contribute to the accurate diagnosis of ruminant streptococcal infections.
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spelling pubmed-68546882019-11-21 Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants Okura, Masatoshi Maruyama, Fumito Ota, Atsushi Tanaka, Takeshi Matoba, Yohei Osawa, Aya Sadaat, Sayed Mushtaq Osaki, Makoto Toyoda, Atsushi Ogura, Yoshitoshi Hayashi, Tetsuya Takamatsu, Daisuke Vet Res Research Article Although Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and some of these isolates were reclassified as the novel species Streptococcus ruminantium. In Japan, biochemically identified S. suis is frequently isolated from diseased ruminants; however, such isolates have not yet been identified accurately, and their aetiological importance in ruminants is unclear. Therefore, to understand the importance of S. suis and S. suis-like bacteria in ruminants, we reclassified S. suis isolates from ruminants according to the updated classification and investigated their genetic diversity. Although both S. suis and S. ruminantium were isolated from healthy and diseased ruminants, most of the isolates from diseased animals were S. ruminantium, implying that S. ruminantium is more likely to be associated with ruminant disease than S. suis. However, the ruminant S. suis and S. ruminantium isolates from diseased animals were classified into diverse genotypes rather than belonging to certain clonal groups. Genome sequence analysis of 20 S. ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. We further developed an S. ruminantium-specific PCR assay to aid in the identification of this bacterium. The information obtained and the method established in this study will contribute to the accurate diagnosis of ruminant streptococcal infections. BioMed Central 2019-11-14 2019 /pmc/articles/PMC6854688/ /pubmed/31727180 http://dx.doi.org/10.1186/s13567-019-0708-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Okura, Masatoshi
Maruyama, Fumito
Ota, Atsushi
Tanaka, Takeshi
Matoba, Yohei
Osawa, Aya
Sadaat, Sayed Mushtaq
Osaki, Makoto
Toyoda, Atsushi
Ogura, Yoshitoshi
Hayashi, Tetsuya
Takamatsu, Daisuke
Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants
title Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants
title_full Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants
title_fullStr Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants
title_full_unstemmed Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants
title_short Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants
title_sort genotypic diversity of streptococcus suis and the s. suis-like bacterium streptococcus ruminantium in ruminants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854688/
https://www.ncbi.nlm.nih.gov/pubmed/31727180
http://dx.doi.org/10.1186/s13567-019-0708-1
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