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Transcriptome Analysis of Hypertrophic Heart Tissues from Murine Transverse Aortic Constriction and Human Aortic Stenosis Reveals Key Genes and Transcription Factors Involved in Cardiac Remodeling Induced by Mechanical Stress

BACKGROUND: Mechanical stress-induced cardiac remodeling that results in heart failure is characterized by transcriptional reprogramming of gene expression. However, a systematic study of genomic changes involved in this process has not been performed to date. To investigate the genomic changes and...

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Autores principales: Yu, Peng, Zhang, Baoli, Liu, Ming, Yu, Ying, Zhao, Ji, Zhang, Chunyu, Li, Yana, Zhang, Lei, Yang, Xue, Jiang, Hong, Zou, Yunzeng, Ge, Junbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854968/
https://www.ncbi.nlm.nih.gov/pubmed/31772688
http://dx.doi.org/10.1155/2019/5058313
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author Yu, Peng
Zhang, Baoli
Liu, Ming
Yu, Ying
Zhao, Ji
Zhang, Chunyu
Li, Yana
Zhang, Lei
Yang, Xue
Jiang, Hong
Zou, Yunzeng
Ge, Junbo
author_facet Yu, Peng
Zhang, Baoli
Liu, Ming
Yu, Ying
Zhao, Ji
Zhang, Chunyu
Li, Yana
Zhang, Lei
Yang, Xue
Jiang, Hong
Zou, Yunzeng
Ge, Junbo
author_sort Yu, Peng
collection PubMed
description BACKGROUND: Mechanical stress-induced cardiac remodeling that results in heart failure is characterized by transcriptional reprogramming of gene expression. However, a systematic study of genomic changes involved in this process has not been performed to date. To investigate the genomic changes and underlying mechanism of cardiac remodeling, we collected and analyzed DNA microarray data for murine transverse aortic constriction (TAC) and human aortic stenosis (AS) from the Gene Expression Omnibus database and the European Bioinformatics Institute. METHODS AND RESULTS: The differential expression genes (DEGs) across the datasets were merged. The Venn diagrams showed that the number of intersections for early and late cardiac remodeling was 74 and 16, respectively. Gene ontology and protein–protein interaction network analysis showed that metabolic changes, cell differentiation and growth, cell cycling, and collagen fibril organization accounted for a great portion of the DEGs in the TAC model, while in AS patients' immune system signaling and cytokine signaling displayed the most significant changes. The intersections between the TAC model and AS patients were few. Nevertheless, the DEGs of the two species shared some common regulatory transcription factors (TFs), including SP1, CEBPB, PPARG, and NFKB1, when the heart was challenged by applied mechanical stress. CONCLUSIONS: This study unravels the complex transcriptome profiles of the heart tissues and highlighting the candidate genes involved in cardiac remodeling induced by mechanical stress may usher in a new era of precision diagnostics and treatment in patients with cardiac remodeling.
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spelling pubmed-68549682019-11-26 Transcriptome Analysis of Hypertrophic Heart Tissues from Murine Transverse Aortic Constriction and Human Aortic Stenosis Reveals Key Genes and Transcription Factors Involved in Cardiac Remodeling Induced by Mechanical Stress Yu, Peng Zhang, Baoli Liu, Ming Yu, Ying Zhao, Ji Zhang, Chunyu Li, Yana Zhang, Lei Yang, Xue Jiang, Hong Zou, Yunzeng Ge, Junbo Dis Markers Research Article BACKGROUND: Mechanical stress-induced cardiac remodeling that results in heart failure is characterized by transcriptional reprogramming of gene expression. However, a systematic study of genomic changes involved in this process has not been performed to date. To investigate the genomic changes and underlying mechanism of cardiac remodeling, we collected and analyzed DNA microarray data for murine transverse aortic constriction (TAC) and human aortic stenosis (AS) from the Gene Expression Omnibus database and the European Bioinformatics Institute. METHODS AND RESULTS: The differential expression genes (DEGs) across the datasets were merged. The Venn diagrams showed that the number of intersections for early and late cardiac remodeling was 74 and 16, respectively. Gene ontology and protein–protein interaction network analysis showed that metabolic changes, cell differentiation and growth, cell cycling, and collagen fibril organization accounted for a great portion of the DEGs in the TAC model, while in AS patients' immune system signaling and cytokine signaling displayed the most significant changes. The intersections between the TAC model and AS patients were few. Nevertheless, the DEGs of the two species shared some common regulatory transcription factors (TFs), including SP1, CEBPB, PPARG, and NFKB1, when the heart was challenged by applied mechanical stress. CONCLUSIONS: This study unravels the complex transcriptome profiles of the heart tissues and highlighting the candidate genes involved in cardiac remodeling induced by mechanical stress may usher in a new era of precision diagnostics and treatment in patients with cardiac remodeling. Hindawi 2019-10-27 /pmc/articles/PMC6854968/ /pubmed/31772688 http://dx.doi.org/10.1155/2019/5058313 Text en Copyright © 2019 Peng Yu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yu, Peng
Zhang, Baoli
Liu, Ming
Yu, Ying
Zhao, Ji
Zhang, Chunyu
Li, Yana
Zhang, Lei
Yang, Xue
Jiang, Hong
Zou, Yunzeng
Ge, Junbo
Transcriptome Analysis of Hypertrophic Heart Tissues from Murine Transverse Aortic Constriction and Human Aortic Stenosis Reveals Key Genes and Transcription Factors Involved in Cardiac Remodeling Induced by Mechanical Stress
title Transcriptome Analysis of Hypertrophic Heart Tissues from Murine Transverse Aortic Constriction and Human Aortic Stenosis Reveals Key Genes and Transcription Factors Involved in Cardiac Remodeling Induced by Mechanical Stress
title_full Transcriptome Analysis of Hypertrophic Heart Tissues from Murine Transverse Aortic Constriction and Human Aortic Stenosis Reveals Key Genes and Transcription Factors Involved in Cardiac Remodeling Induced by Mechanical Stress
title_fullStr Transcriptome Analysis of Hypertrophic Heart Tissues from Murine Transverse Aortic Constriction and Human Aortic Stenosis Reveals Key Genes and Transcription Factors Involved in Cardiac Remodeling Induced by Mechanical Stress
title_full_unstemmed Transcriptome Analysis of Hypertrophic Heart Tissues from Murine Transverse Aortic Constriction and Human Aortic Stenosis Reveals Key Genes and Transcription Factors Involved in Cardiac Remodeling Induced by Mechanical Stress
title_short Transcriptome Analysis of Hypertrophic Heart Tissues from Murine Transverse Aortic Constriction and Human Aortic Stenosis Reveals Key Genes and Transcription Factors Involved in Cardiac Remodeling Induced by Mechanical Stress
title_sort transcriptome analysis of hypertrophic heart tissues from murine transverse aortic constriction and human aortic stenosis reveals key genes and transcription factors involved in cardiac remodeling induced by mechanical stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854968/
https://www.ncbi.nlm.nih.gov/pubmed/31772688
http://dx.doi.org/10.1155/2019/5058313
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