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Immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis

Background: Psoriasis is the prime example of psoriasiform tissue pattern and should be differentiated from other psoriasiform dermatoses both clinically and histopathologically. Aim: To evaluate immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis for diag...

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Autores principales: Ramezani, Mazaher, Shamshiri, Atefeh, Zavattaro, Elisa, Khazaei, Sedigheh, Rezaei, Mansour, Mahmoodi, Rozhano, Sadeghi, Masoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6855186/
https://www.ncbi.nlm.nih.gov/pubmed/31724940
http://dx.doi.org/10.1051/bmdcn/2019090426
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author Ramezani, Mazaher
Shamshiri, Atefeh
Zavattaro, Elisa
Khazaei, Sedigheh
Rezaei, Mansour
Mahmoodi, Rozhano
Sadeghi, Masoud
author_facet Ramezani, Mazaher
Shamshiri, Atefeh
Zavattaro, Elisa
Khazaei, Sedigheh
Rezaei, Mansour
Mahmoodi, Rozhano
Sadeghi, Masoud
author_sort Ramezani, Mazaher
collection PubMed
description Background: Psoriasis is the prime example of psoriasiform tissue pattern and should be differentiated from other psoriasiform dermatoses both clinically and histopathologically. Aim: To evaluate immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis for diagnostic purposes. Methods: An analytical cross-sectional study was performed on the paraffin blocks of 60 psoriasis and 31 psoriasiform dermatitis patients between 2014 and 2017. The selected formalin-fixed paraffin-embedded tissues from each biopsy specimen were cut into 4-micron sections. Initial sections were stained by hematoxylin and eosin staining. Primary antihuman antibodies against P53, Ki-67, and CD34 were applied. Positive control samples for biomarkers were received from former strongly positive samples of papillary endothelial hyperplasia, high grade lymphoma, and breast ductal carcinoma for CD34, Ki-67, and P53, respectively. Results: Out of 60 psoriasis patients, 56.7% were men, with the mean age of 36.8 years. From 31 psoriasiform patients, 45.2% were men, with the mean age of 37.5 years. Both groups were matched in terms of sex and age. The mean staining of three markers was more significant in psoriasiform dermatitis than psoriasis. Conclusion: In spite of some other researches, the present study showed expression of P53, Ki-67, and CD34 biomarkers were significantly higher in psoriasiform dermatitis than psoriasis.
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spelling pubmed-68551862019-12-18 Immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis Ramezani, Mazaher Shamshiri, Atefeh Zavattaro, Elisa Khazaei, Sedigheh Rezaei, Mansour Mahmoodi, Rozhano Sadeghi, Masoud Biomedicine (Taipei) Original Article Background: Psoriasis is the prime example of psoriasiform tissue pattern and should be differentiated from other psoriasiform dermatoses both clinically and histopathologically. Aim: To evaluate immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis for diagnostic purposes. Methods: An analytical cross-sectional study was performed on the paraffin blocks of 60 psoriasis and 31 psoriasiform dermatitis patients between 2014 and 2017. The selected formalin-fixed paraffin-embedded tissues from each biopsy specimen were cut into 4-micron sections. Initial sections were stained by hematoxylin and eosin staining. Primary antihuman antibodies against P53, Ki-67, and CD34 were applied. Positive control samples for biomarkers were received from former strongly positive samples of papillary endothelial hyperplasia, high grade lymphoma, and breast ductal carcinoma for CD34, Ki-67, and P53, respectively. Results: Out of 60 psoriasis patients, 56.7% were men, with the mean age of 36.8 years. From 31 psoriasiform patients, 45.2% were men, with the mean age of 37.5 years. Both groups were matched in terms of sex and age. The mean staining of three markers was more significant in psoriasiform dermatitis than psoriasis. Conclusion: In spite of some other researches, the present study showed expression of P53, Ki-67, and CD34 biomarkers were significantly higher in psoriasiform dermatitis than psoriasis. EDP Sciences 2019-11-14 /pmc/articles/PMC6855186/ /pubmed/31724940 http://dx.doi.org/10.1051/bmdcn/2019090426 Text en © Author(s) 2019. This article is published with open access by China Medical University Open Access This article is distributed under terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) which permits any use, distribution, and reproduction in any medium, provided original author(s) and source are credited.
spellingShingle Original Article
Ramezani, Mazaher
Shamshiri, Atefeh
Zavattaro, Elisa
Khazaei, Sedigheh
Rezaei, Mansour
Mahmoodi, Rozhano
Sadeghi, Masoud
Immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis
title Immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis
title_full Immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis
title_fullStr Immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis
title_full_unstemmed Immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis
title_short Immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis
title_sort immunohistochemical expression of p53, ki-67, and cd34 in psoriasis and psoriasiform dermatitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6855186/
https://www.ncbi.nlm.nih.gov/pubmed/31724940
http://dx.doi.org/10.1051/bmdcn/2019090426
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