LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer

Long non-coding RNAs (lncRNAs) are increasingly recognized as important regulators in tumor development. This study aims to investigate the potential role oflncRNALEF1-AS1, in the progression of lung cancer. Quantitative real-time PCR (qRT-PCR) and western blot assays showed that LEF1-AS1 was upregu...

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Autores principales: Wang, Ansheng, Zhao, Chengling, Gao, Yuan, Duan, Guixin, Yang, Yuming, Fan, Bo, Wang, Xiaojing, Wang, Kangwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2019
Materias:
Preclinical Studies
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856024/
https://www.ncbi.nlm.nih.gov/pubmed/30734202
http://dx.doi.org/10.1007/s10637-018-00721-z
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author Wang, Ansheng
Zhao, Chengling
Gao, Yuan
Duan, Guixin
Yang, Yuming
Fan, Bo
Wang, Xiaojing
Wang, Kangwu
author_facet Wang, Ansheng
Zhao, Chengling
Gao, Yuan
Duan, Guixin
Yang, Yuming
Fan, Bo
Wang, Xiaojing
Wang, Kangwu
author_sort Wang, Ansheng
collection PubMed
description Long non-coding RNAs (lncRNAs) are increasingly recognized as important regulators in tumor development. This study aims to investigate the potential role oflncRNALEF1-AS1, in the progression of lung cancer. Quantitative real-time PCR (qRT-PCR) and western blot assays showed that LEF1-AS1 was upregulated while miR-544a was downregulated in lung cancer specimens and cells. Overexpression of LEF1-AS1 led to the enhancement of cell proliferation and invasion, revealed by CCK-8 assay and transwell assay. A negative correlation was found between LEF1-AS1 and miR-544a. BLAST analysis and dual-luciferase assay confirmed that FOXP1 is a downstream effector of miR-544a. Therefore, the LEF1-AS1/miR-544a/FOXP1 axis is an important contributor to lung cancer progression. Collectively, our novel data uncovers a new mechanism that governs tumor progression in lung cancer and provides new targets that may be used for disease monitoring and therapeutic intervention of lung cancer.
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spelling pubmed-68560242019-12-03 LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer Wang, Ansheng Zhao, Chengling Gao, Yuan Duan, Guixin Yang, Yuming Fan, Bo Wang, Xiaojing Wang, Kangwu Invest New Drugs Preclinical Studies Long non-coding RNAs (lncRNAs) are increasingly recognized as important regulators in tumor development. This study aims to investigate the potential role oflncRNALEF1-AS1, in the progression of lung cancer. Quantitative real-time PCR (qRT-PCR) and western blot assays showed that LEF1-AS1 was upregulated while miR-544a was downregulated in lung cancer specimens and cells. Overexpression of LEF1-AS1 led to the enhancement of cell proliferation and invasion, revealed by CCK-8 assay and transwell assay. A negative correlation was found between LEF1-AS1 and miR-544a. BLAST analysis and dual-luciferase assay confirmed that FOXP1 is a downstream effector of miR-544a. Therefore, the LEF1-AS1/miR-544a/FOXP1 axis is an important contributor to lung cancer progression. Collectively, our novel data uncovers a new mechanism that governs tumor progression in lung cancer and provides new targets that may be used for disease monitoring and therapeutic intervention of lung cancer. Springer US 2019-02-08 2019 /pmc/articles/PMC6856024/ /pubmed/30734202 http://dx.doi.org/10.1007/s10637-018-00721-z Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Preclinical Studies
Wang, Ansheng
Zhao, Chengling
Gao, Yuan
Duan, Guixin
Yang, Yuming
Fan, Bo
Wang, Xiaojing
Wang, Kangwu
LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer
title LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer
title_full LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer
title_fullStr LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer
title_full_unstemmed LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer
title_short LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer
title_sort lef1-as1 contributes to proliferation and invasion through regulating mir-544a/ foxp1 axis in lung cancer
topic Preclinical Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856024/
https://www.ncbi.nlm.nih.gov/pubmed/30734202
http://dx.doi.org/10.1007/s10637-018-00721-z
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