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p53/PCDH17/Beclin-1 Proteins as Prognostic Predictors for Urinary Bladder Cancer
Purpose: To determine whether p53, PCDH17, Beclin-1 expression is associated with clinicopathological characteristics of bladder cancer. Materials and Methods: 75 patients with non-muscle-invasive and muscle-invasive bladder cancer were included. Immunohistochemical staining for p53, PCDH17 and Becl...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856748/ https://www.ncbi.nlm.nih.gov/pubmed/31772653 http://dx.doi.org/10.7150/jca.37335 |
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author | Chen, Liuxi Liu, Ying Zhang, Qi Zhang, Mingming Han, Xuemeng Li, Qiujie Xie, Tian Wu, Qibiao Sui, Xinbing |
author_facet | Chen, Liuxi Liu, Ying Zhang, Qi Zhang, Mingming Han, Xuemeng Li, Qiujie Xie, Tian Wu, Qibiao Sui, Xinbing |
author_sort | Chen, Liuxi |
collection | PubMed |
description | Purpose: To determine whether p53, PCDH17, Beclin-1 expression is associated with clinicopathological characteristics of bladder cancer. Materials and Methods: 75 patients with non-muscle-invasive and muscle-invasive bladder cancer were included. Immunohistochemical staining for p53, PCDH17 and Beclin-1 were carried out on the same paraffin-embedded blocks serial sections of these patients who underwent surgery between 2010 and 2015. In addition, p53 gene mutations in these tumors were screened by DNA sequencing. Results: Forty-nine (66.7%) of 75 tumors had p53 gene mutations detected by DNA sequencing method. Of these tumors, 43 (86.0%) exhibited p53 high expression. Furthermore, p53 mutation and low expression of PCDH17 were significantly associated with muscle-invasive bladder cancer. Beclin-1 was also strongly associated with T stage. The p53 mutation, the expression of p53 and PCDH17 were significantly associated with survival from bladder cancer. In addition, patients with p53 high-expression or p53 mutation, PCDH17 low-expression and Beclin-1 low-expression significantly had a poor prognosis. Conclusions: Use of a DNA sequencing method to detect p53 gene mutations was consistent with an immunohistochemical method to detect p53 alterations. In conjunction with levels of p53/PCDH17/Beclin-1, p53 and PCDH17 were independently associated with prognosis; Beclin-1 only had a tendency towards overall survival. p53/PCDH17/Beclin-1 phenotype seems to play a more important role than p53 expression in bladder cancer outcome. It is also identified that p53/PCDH17, p53/Beclin-1 or PCDH17/Beclin-1 all have a cooperative and synergistic effect, which may provide us the potential biomarker for bladder cancer patients. |
format | Online Article Text |
id | pubmed-6856748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-68567482019-11-26 p53/PCDH17/Beclin-1 Proteins as Prognostic Predictors for Urinary Bladder Cancer Chen, Liuxi Liu, Ying Zhang, Qi Zhang, Mingming Han, Xuemeng Li, Qiujie Xie, Tian Wu, Qibiao Sui, Xinbing J Cancer Research Paper Purpose: To determine whether p53, PCDH17, Beclin-1 expression is associated with clinicopathological characteristics of bladder cancer. Materials and Methods: 75 patients with non-muscle-invasive and muscle-invasive bladder cancer were included. Immunohistochemical staining for p53, PCDH17 and Beclin-1 were carried out on the same paraffin-embedded blocks serial sections of these patients who underwent surgery between 2010 and 2015. In addition, p53 gene mutations in these tumors were screened by DNA sequencing. Results: Forty-nine (66.7%) of 75 tumors had p53 gene mutations detected by DNA sequencing method. Of these tumors, 43 (86.0%) exhibited p53 high expression. Furthermore, p53 mutation and low expression of PCDH17 were significantly associated with muscle-invasive bladder cancer. Beclin-1 was also strongly associated with T stage. The p53 mutation, the expression of p53 and PCDH17 were significantly associated with survival from bladder cancer. In addition, patients with p53 high-expression or p53 mutation, PCDH17 low-expression and Beclin-1 low-expression significantly had a poor prognosis. Conclusions: Use of a DNA sequencing method to detect p53 gene mutations was consistent with an immunohistochemical method to detect p53 alterations. In conjunction with levels of p53/PCDH17/Beclin-1, p53 and PCDH17 were independently associated with prognosis; Beclin-1 only had a tendency towards overall survival. p53/PCDH17/Beclin-1 phenotype seems to play a more important role than p53 expression in bladder cancer outcome. It is also identified that p53/PCDH17, p53/Beclin-1 or PCDH17/Beclin-1 all have a cooperative and synergistic effect, which may provide us the potential biomarker for bladder cancer patients. Ivyspring International Publisher 2019-10-15 /pmc/articles/PMC6856748/ /pubmed/31772653 http://dx.doi.org/10.7150/jca.37335 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Chen, Liuxi Liu, Ying Zhang, Qi Zhang, Mingming Han, Xuemeng Li, Qiujie Xie, Tian Wu, Qibiao Sui, Xinbing p53/PCDH17/Beclin-1 Proteins as Prognostic Predictors for Urinary Bladder Cancer |
title | p53/PCDH17/Beclin-1 Proteins as Prognostic Predictors for Urinary Bladder Cancer |
title_full | p53/PCDH17/Beclin-1 Proteins as Prognostic Predictors for Urinary Bladder Cancer |
title_fullStr | p53/PCDH17/Beclin-1 Proteins as Prognostic Predictors for Urinary Bladder Cancer |
title_full_unstemmed | p53/PCDH17/Beclin-1 Proteins as Prognostic Predictors for Urinary Bladder Cancer |
title_short | p53/PCDH17/Beclin-1 Proteins as Prognostic Predictors for Urinary Bladder Cancer |
title_sort | p53/pcdh17/beclin-1 proteins as prognostic predictors for urinary bladder cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856748/ https://www.ncbi.nlm.nih.gov/pubmed/31772653 http://dx.doi.org/10.7150/jca.37335 |
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