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Biochemical and biomechanical comparisions of decellularized scaffolds derived from porcine subcutaneous and visceral adipose tissue

Decellularized adipose tissue (DAT) is a promising biomaterial for adipose tissue engineering. However, there is a lack of research of DAT prepared from xenogeneic porcine adipose tissue. This study aimed to compare the adipogenic ability of DAT derived from porcine subcutaneous (SDAT) and visceral...

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Autores principales: Lin, Maohui, Ge, Jinbo, Wang, Xuecen, Dong, Ziqing, Xing, Malcolm, Lu, Feng, He, Yunfan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856974/
https://www.ncbi.nlm.nih.gov/pubmed/31762987
http://dx.doi.org/10.1177/2041731419888168
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author Lin, Maohui
Ge, Jinbo
Wang, Xuecen
Dong, Ziqing
Xing, Malcolm
Lu, Feng
He, Yunfan
author_facet Lin, Maohui
Ge, Jinbo
Wang, Xuecen
Dong, Ziqing
Xing, Malcolm
Lu, Feng
He, Yunfan
author_sort Lin, Maohui
collection PubMed
description Decellularized adipose tissue (DAT) is a promising biomaterial for adipose tissue engineering. However, there is a lack of research of DAT prepared from xenogeneic porcine adipose tissue. This study aimed to compare the adipogenic ability of DAT derived from porcine subcutaneous (SDAT) and visceral adipose tissue (VDAT). The retention of key collagen in decellularized matrix was analysed to study the biochemical properties of SDAT and VDAT. For the biomechanical study, both DAT materials were fabricated into three-dimensional (3D) porous scaffolds for rheology and compressive tests. Human adipose-derived stem cells (ADSCs) were cultured on both scaffolds to further investigate the effect of matrix stiffness on cellular morphology and on adipogenic differentiation. ADSCs cultured on soft VDAT exhibited significantly reduced cellular area and upregulated adipogenic markers compared to those cultured on SDAT. In vivo results revealed higher adipose regeneration in the VDAT compared to the SDAT. This study further demonstrated that the relative expression of collagen IV and laminin was significantly higher in VDAT than in SDAT, while the collagen I expression and matrix stiffness of SDAT was significantly higher in comparison to VDAT. This result suggested that porcine adipose tissue could serve as a promising candidate for preparing DAT.
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spelling pubmed-68569742019-11-22 Biochemical and biomechanical comparisions of decellularized scaffolds derived from porcine subcutaneous and visceral adipose tissue Lin, Maohui Ge, Jinbo Wang, Xuecen Dong, Ziqing Xing, Malcolm Lu, Feng He, Yunfan J Tissue Eng Acellular approaches for regenerative medicine Decellularized adipose tissue (DAT) is a promising biomaterial for adipose tissue engineering. However, there is a lack of research of DAT prepared from xenogeneic porcine adipose tissue. This study aimed to compare the adipogenic ability of DAT derived from porcine subcutaneous (SDAT) and visceral adipose tissue (VDAT). The retention of key collagen in decellularized matrix was analysed to study the biochemical properties of SDAT and VDAT. For the biomechanical study, both DAT materials were fabricated into three-dimensional (3D) porous scaffolds for rheology and compressive tests. Human adipose-derived stem cells (ADSCs) were cultured on both scaffolds to further investigate the effect of matrix stiffness on cellular morphology and on adipogenic differentiation. ADSCs cultured on soft VDAT exhibited significantly reduced cellular area and upregulated adipogenic markers compared to those cultured on SDAT. In vivo results revealed higher adipose regeneration in the VDAT compared to the SDAT. This study further demonstrated that the relative expression of collagen IV and laminin was significantly higher in VDAT than in SDAT, while the collagen I expression and matrix stiffness of SDAT was significantly higher in comparison to VDAT. This result suggested that porcine adipose tissue could serve as a promising candidate for preparing DAT. SAGE Publications 2019-11-14 /pmc/articles/PMC6856974/ /pubmed/31762987 http://dx.doi.org/10.1177/2041731419888168 Text en © The Author(s) 2019 http://www.creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Acellular approaches for regenerative medicine
Lin, Maohui
Ge, Jinbo
Wang, Xuecen
Dong, Ziqing
Xing, Malcolm
Lu, Feng
He, Yunfan
Biochemical and biomechanical comparisions of decellularized scaffolds derived from porcine subcutaneous and visceral adipose tissue
title Biochemical and biomechanical comparisions of decellularized scaffolds derived from porcine subcutaneous and visceral adipose tissue
title_full Biochemical and biomechanical comparisions of decellularized scaffolds derived from porcine subcutaneous and visceral adipose tissue
title_fullStr Biochemical and biomechanical comparisions of decellularized scaffolds derived from porcine subcutaneous and visceral adipose tissue
title_full_unstemmed Biochemical and biomechanical comparisions of decellularized scaffolds derived from porcine subcutaneous and visceral adipose tissue
title_short Biochemical and biomechanical comparisions of decellularized scaffolds derived from porcine subcutaneous and visceral adipose tissue
title_sort biochemical and biomechanical comparisions of decellularized scaffolds derived from porcine subcutaneous and visceral adipose tissue
topic Acellular approaches for regenerative medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856974/
https://www.ncbi.nlm.nih.gov/pubmed/31762987
http://dx.doi.org/10.1177/2041731419888168
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