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Multi‐component meningococcal serogroup B (MenB)‐4C vaccine induces effective opsonophagocytic killing in children with a complement deficiency

Vaccination against meningococcal serogroup B is recommended for patients with a complement deficiency; however, although immunogenicity in this patient group has been shown, efficacy has not yet been established. In this study, we collected serum from children with a complement deficiency in the al...

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Autores principales: van den Broek, B., van Els, C. A. C. M., Kuipers, B., van Aerde, K., Henriet, S. S., de Groot, R., de Jonge, M. I., Langereis, J. D., van der Flier, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857189/
https://www.ncbi.nlm.nih.gov/pubmed/31487400
http://dx.doi.org/10.1111/cei.13368
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author van den Broek, B.
van Els, C. A. C. M.
Kuipers, B.
van Aerde, K.
Henriet, S. S.
de Groot, R.
de Jonge, M. I.
Langereis, J. D.
van der Flier, M.
author_facet van den Broek, B.
van Els, C. A. C. M.
Kuipers, B.
van Aerde, K.
Henriet, S. S.
de Groot, R.
de Jonge, M. I.
Langereis, J. D.
van der Flier, M.
author_sort van den Broek, B.
collection PubMed
description Vaccination against meningococcal serogroup B is recommended for patients with a complement deficiency; however, although immunogenicity in this patient group has been shown, efficacy has not yet been established. In this study, we collected serum from children with a complement deficiency in the alternative pathway or in late terminal pathway before and after vaccination with multi‐component meningococcal serogroup B (MenB)‐4C. MenB‐4C is a multi‐component, protein‐based vaccine against MenB consisting of factor H‐binding protein, Neisserial heparin‐binding protein, Neisserial adhesion A and outer membrane vesicles containing Porin A. We assessed the vaccine immunogenicity and vaccine‐mediated protection by a whole cell enzyme‐linked immunosorbent assay with Neisseria meningitidis serogroup B strains H44/76, 5/99 and NZ98/254, which shows that vaccination induced antibody titers against meningococcus. We show that the classical serum bactericidal activity assay with exogenous serum indicates the presence of vaccine‐induced antibodies and capacity to activate complement‐mediated pathogen lysis. However, in children with a late terminal pathway deficiency, no complement‐mediated pathogen lysis was observed when autologous serum was applied in the serum bactericidal activity assay, demonstrating a lack of serum bactericidal activity in children with complement deficiencies. However, MenB‐4C vaccination still induced effective complement‐dependent opsonophagocytic killing against N. meningitidis serogroup B in reconstituted whole blood with autologous serum from children with an alternative pathway or late terminal pathway deficiency. These findings support the recommendation to vaccinate all complement‐deficient children against MenB.
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spelling pubmed-68571892019-12-12 Multi‐component meningococcal serogroup B (MenB)‐4C vaccine induces effective opsonophagocytic killing in children with a complement deficiency van den Broek, B. van Els, C. A. C. M. Kuipers, B. van Aerde, K. Henriet, S. S. de Groot, R. de Jonge, M. I. Langereis, J. D. van der Flier, M. Clin Exp Immunol Original Articles Vaccination against meningococcal serogroup B is recommended for patients with a complement deficiency; however, although immunogenicity in this patient group has been shown, efficacy has not yet been established. In this study, we collected serum from children with a complement deficiency in the alternative pathway or in late terminal pathway before and after vaccination with multi‐component meningococcal serogroup B (MenB)‐4C. MenB‐4C is a multi‐component, protein‐based vaccine against MenB consisting of factor H‐binding protein, Neisserial heparin‐binding protein, Neisserial adhesion A and outer membrane vesicles containing Porin A. We assessed the vaccine immunogenicity and vaccine‐mediated protection by a whole cell enzyme‐linked immunosorbent assay with Neisseria meningitidis serogroup B strains H44/76, 5/99 and NZ98/254, which shows that vaccination induced antibody titers against meningococcus. We show that the classical serum bactericidal activity assay with exogenous serum indicates the presence of vaccine‐induced antibodies and capacity to activate complement‐mediated pathogen lysis. However, in children with a late terminal pathway deficiency, no complement‐mediated pathogen lysis was observed when autologous serum was applied in the serum bactericidal activity assay, demonstrating a lack of serum bactericidal activity in children with complement deficiencies. However, MenB‐4C vaccination still induced effective complement‐dependent opsonophagocytic killing against N. meningitidis serogroup B in reconstituted whole blood with autologous serum from children with an alternative pathway or late terminal pathway deficiency. These findings support the recommendation to vaccinate all complement‐deficient children against MenB. John Wiley and Sons Inc. 2019-10-01 2019-12 /pmc/articles/PMC6857189/ /pubmed/31487400 http://dx.doi.org/10.1111/cei.13368 Text en © 2019 The Authors. Clinical & Experimental Immunology published by John Wiley & Sons Ltd on behalf of British Society for Immunology This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
van den Broek, B.
van Els, C. A. C. M.
Kuipers, B.
van Aerde, K.
Henriet, S. S.
de Groot, R.
de Jonge, M. I.
Langereis, J. D.
van der Flier, M.
Multi‐component meningococcal serogroup B (MenB)‐4C vaccine induces effective opsonophagocytic killing in children with a complement deficiency
title Multi‐component meningococcal serogroup B (MenB)‐4C vaccine induces effective opsonophagocytic killing in children with a complement deficiency
title_full Multi‐component meningococcal serogroup B (MenB)‐4C vaccine induces effective opsonophagocytic killing in children with a complement deficiency
title_fullStr Multi‐component meningococcal serogroup B (MenB)‐4C vaccine induces effective opsonophagocytic killing in children with a complement deficiency
title_full_unstemmed Multi‐component meningococcal serogroup B (MenB)‐4C vaccine induces effective opsonophagocytic killing in children with a complement deficiency
title_short Multi‐component meningococcal serogroup B (MenB)‐4C vaccine induces effective opsonophagocytic killing in children with a complement deficiency
title_sort multi‐component meningococcal serogroup b (menb)‐4c vaccine induces effective opsonophagocytic killing in children with a complement deficiency
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857189/
https://www.ncbi.nlm.nih.gov/pubmed/31487400
http://dx.doi.org/10.1111/cei.13368
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