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Biophysics of Cell-Substrate Interactions Under Shear
Cells adhere to substrates through mechanosensitive focal adhesion complexes. Measurements that probe how cells detach from substrates when they experience an applied force connect molecular-scale aspects of cell adhesion with the biophysical properties of adherent cells. Such forces can be applied...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857480/ https://www.ncbi.nlm.nih.gov/pubmed/31781558 http://dx.doi.org/10.3389/fcell.2019.00251 |
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author | Paddillaya, Neha Mishra, Ashish Kondaiah, Paturu Pullarkat, Pramod Menon, Gautam I. Gundiah, Namrata |
author_facet | Paddillaya, Neha Mishra, Ashish Kondaiah, Paturu Pullarkat, Pramod Menon, Gautam I. Gundiah, Namrata |
author_sort | Paddillaya, Neha |
collection | PubMed |
description | Cells adhere to substrates through mechanosensitive focal adhesion complexes. Measurements that probe how cells detach from substrates when they experience an applied force connect molecular-scale aspects of cell adhesion with the biophysical properties of adherent cells. Such forces can be applied through shear devices that flow fluid in a controlled manner across cells. The signaling pathways associated with focal adhesions, in particular those that involve integrins and receptor tyrosine kinases, are complex, receiving mechano-chemical feedback from the sensing of substrate stiffness as well as of external forces. This article reviews the signaling processes involved in mechanosensing and mechanotransduction during cell-substrate interactions, describing the role such signaling plays in cancer metastasis. We examine some recent progress in quantifying the strength of these interactions, describing a novel fluid shear device that allows for the visualization of the cell and its sub-cellular structures under a shear flow. We also summarize related results from a biophysical model for cellular de-adhesion induced by applied forces. Quantifying cell-substrate adhesions under shear should aid in the development of mechano-diagnostic techniques for diseases in which cell-adhesion is mis-regulated, such as cancers. |
format | Online Article Text |
id | pubmed-6857480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68574802019-11-28 Biophysics of Cell-Substrate Interactions Under Shear Paddillaya, Neha Mishra, Ashish Kondaiah, Paturu Pullarkat, Pramod Menon, Gautam I. Gundiah, Namrata Front Cell Dev Biol Cell and Developmental Biology Cells adhere to substrates through mechanosensitive focal adhesion complexes. Measurements that probe how cells detach from substrates when they experience an applied force connect molecular-scale aspects of cell adhesion with the biophysical properties of adherent cells. Such forces can be applied through shear devices that flow fluid in a controlled manner across cells. The signaling pathways associated with focal adhesions, in particular those that involve integrins and receptor tyrosine kinases, are complex, receiving mechano-chemical feedback from the sensing of substrate stiffness as well as of external forces. This article reviews the signaling processes involved in mechanosensing and mechanotransduction during cell-substrate interactions, describing the role such signaling plays in cancer metastasis. We examine some recent progress in quantifying the strength of these interactions, describing a novel fluid shear device that allows for the visualization of the cell and its sub-cellular structures under a shear flow. We also summarize related results from a biophysical model for cellular de-adhesion induced by applied forces. Quantifying cell-substrate adhesions under shear should aid in the development of mechano-diagnostic techniques for diseases in which cell-adhesion is mis-regulated, such as cancers. Frontiers Media S.A. 2019-11-08 /pmc/articles/PMC6857480/ /pubmed/31781558 http://dx.doi.org/10.3389/fcell.2019.00251 Text en Copyright © 2019 Paddillaya, Mishra, Kondaiah, Pullarkat, Menon and Gundiah. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Paddillaya, Neha Mishra, Ashish Kondaiah, Paturu Pullarkat, Pramod Menon, Gautam I. Gundiah, Namrata Biophysics of Cell-Substrate Interactions Under Shear |
title | Biophysics of Cell-Substrate Interactions Under Shear |
title_full | Biophysics of Cell-Substrate Interactions Under Shear |
title_fullStr | Biophysics of Cell-Substrate Interactions Under Shear |
title_full_unstemmed | Biophysics of Cell-Substrate Interactions Under Shear |
title_short | Biophysics of Cell-Substrate Interactions Under Shear |
title_sort | biophysics of cell-substrate interactions under shear |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857480/ https://www.ncbi.nlm.nih.gov/pubmed/31781558 http://dx.doi.org/10.3389/fcell.2019.00251 |
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