Cargando…
The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing
In humans, zinc finger protein 671 (ZNF671) is a type of transcription factor. However, the contribution of tumor heterogeneity to the functional role of ZNF671 remains unknown. The present study aimed to determine the functional states of ZNF671 in cancer single cells based on single-cell sequencin...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857622/ https://www.ncbi.nlm.nih.gov/pubmed/31781507 http://dx.doi.org/10.3389/fonc.2019.01214 |
_version_ | 1783470803610763264 |
---|---|
author | Zhang, Jian Luo, Jianli Jiang, Huali Xie, Tao Zheng, Jieling Tian, Yunhong Li, Rong Wang, Baiyao Lin, Jie Xu, Anan Huang, Xiaoting Yuan, Yawei |
author_facet | Zhang, Jian Luo, Jianli Jiang, Huali Xie, Tao Zheng, Jieling Tian, Yunhong Li, Rong Wang, Baiyao Lin, Jie Xu, Anan Huang, Xiaoting Yuan, Yawei |
author_sort | Zhang, Jian |
collection | PubMed |
description | In humans, zinc finger protein 671 (ZNF671) is a type of transcription factor. However, the contribution of tumor heterogeneity to the functional role of ZNF671 remains unknown. The present study aimed to determine the functional states of ZNF671 in cancer single cells based on single-cell sequencing datasets (scRNA-seq). We collected cancer-related ZNF671 scRNA-seq datasets and analyzed ZNF671 in the datasets. We evaluated 14 functional states of ZNF671 in cancers and performed ZNF671 expression and function state correlation analysis. We further applied t-distributed stochastic neighbor embedding to describe the distribution of cancer cells and to explore the functional state of ZNF671 in cancer subgroups. We found that ZNF671 was downregulated in eight cancer-related ZNF671 scRNA-seq datasets. Functional analysis identified that ZNF671 might play a tumor suppressor role in cancer. The heterogeneous functional states of cell subgroups and correlation analysis showed that ZNF671 played tumor suppressor roles in heterogeneous cancer cell populations. Western blot and transwell assays identified that ZNF671 inhibited EMT, migration, and invasion of CNS cancers, lung cancer, melanoma, and breast carcinoma in vitro. These results from cancer single-cell sequencing indicated that ZNF671 played a tumor suppressor role in multiple tumors and may provide us with new insights into the role of ZNF671 for cancer treatment. |
format | Online Article Text |
id | pubmed-6857622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68576222019-11-28 The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing Zhang, Jian Luo, Jianli Jiang, Huali Xie, Tao Zheng, Jieling Tian, Yunhong Li, Rong Wang, Baiyao Lin, Jie Xu, Anan Huang, Xiaoting Yuan, Yawei Front Oncol Oncology In humans, zinc finger protein 671 (ZNF671) is a type of transcription factor. However, the contribution of tumor heterogeneity to the functional role of ZNF671 remains unknown. The present study aimed to determine the functional states of ZNF671 in cancer single cells based on single-cell sequencing datasets (scRNA-seq). We collected cancer-related ZNF671 scRNA-seq datasets and analyzed ZNF671 in the datasets. We evaluated 14 functional states of ZNF671 in cancers and performed ZNF671 expression and function state correlation analysis. We further applied t-distributed stochastic neighbor embedding to describe the distribution of cancer cells and to explore the functional state of ZNF671 in cancer subgroups. We found that ZNF671 was downregulated in eight cancer-related ZNF671 scRNA-seq datasets. Functional analysis identified that ZNF671 might play a tumor suppressor role in cancer. The heterogeneous functional states of cell subgroups and correlation analysis showed that ZNF671 played tumor suppressor roles in heterogeneous cancer cell populations. Western blot and transwell assays identified that ZNF671 inhibited EMT, migration, and invasion of CNS cancers, lung cancer, melanoma, and breast carcinoma in vitro. These results from cancer single-cell sequencing indicated that ZNF671 played a tumor suppressor role in multiple tumors and may provide us with new insights into the role of ZNF671 for cancer treatment. Frontiers Media S.A. 2019-11-08 /pmc/articles/PMC6857622/ /pubmed/31781507 http://dx.doi.org/10.3389/fonc.2019.01214 Text en Copyright © 2019 Zhang, Luo, Jiang, Xie, Zheng, Tian, Li, Wang, Lin, Xu, Huang and Yuan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Zhang, Jian Luo, Jianli Jiang, Huali Xie, Tao Zheng, Jieling Tian, Yunhong Li, Rong Wang, Baiyao Lin, Jie Xu, Anan Huang, Xiaoting Yuan, Yawei The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing |
title | The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing |
title_full | The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing |
title_fullStr | The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing |
title_full_unstemmed | The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing |
title_short | The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing |
title_sort | tumor suppressor role of zinc finger protein 671 (znf671) in multiple tumors based on cancer single-cell sequencing |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857622/ https://www.ncbi.nlm.nih.gov/pubmed/31781507 http://dx.doi.org/10.3389/fonc.2019.01214 |
work_keys_str_mv | AT zhangjian thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT luojianli thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT jianghuali thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT xietao thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT zhengjieling thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT tianyunhong thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT lirong thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT wangbaiyao thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT linjie thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT xuanan thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT huangxiaoting thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT yuanyawei thetumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT zhangjian tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT luojianli tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT jianghuali tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT xietao tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT zhengjieling tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT tianyunhong tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT lirong tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT wangbaiyao tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT linjie tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT xuanan tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT huangxiaoting tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing AT yuanyawei tumorsuppressorroleofzincfingerprotein671znf671inmultipletumorsbasedoncancersinglecellsequencing |