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The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing

In humans, zinc finger protein 671 (ZNF671) is a type of transcription factor. However, the contribution of tumor heterogeneity to the functional role of ZNF671 remains unknown. The present study aimed to determine the functional states of ZNF671 in cancer single cells based on single-cell sequencin...

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Autores principales: Zhang, Jian, Luo, Jianli, Jiang, Huali, Xie, Tao, Zheng, Jieling, Tian, Yunhong, Li, Rong, Wang, Baiyao, Lin, Jie, Xu, Anan, Huang, Xiaoting, Yuan, Yawei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857622/
https://www.ncbi.nlm.nih.gov/pubmed/31781507
http://dx.doi.org/10.3389/fonc.2019.01214
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author Zhang, Jian
Luo, Jianli
Jiang, Huali
Xie, Tao
Zheng, Jieling
Tian, Yunhong
Li, Rong
Wang, Baiyao
Lin, Jie
Xu, Anan
Huang, Xiaoting
Yuan, Yawei
author_facet Zhang, Jian
Luo, Jianli
Jiang, Huali
Xie, Tao
Zheng, Jieling
Tian, Yunhong
Li, Rong
Wang, Baiyao
Lin, Jie
Xu, Anan
Huang, Xiaoting
Yuan, Yawei
author_sort Zhang, Jian
collection PubMed
description In humans, zinc finger protein 671 (ZNF671) is a type of transcription factor. However, the contribution of tumor heterogeneity to the functional role of ZNF671 remains unknown. The present study aimed to determine the functional states of ZNF671 in cancer single cells based on single-cell sequencing datasets (scRNA-seq). We collected cancer-related ZNF671 scRNA-seq datasets and analyzed ZNF671 in the datasets. We evaluated 14 functional states of ZNF671 in cancers and performed ZNF671 expression and function state correlation analysis. We further applied t-distributed stochastic neighbor embedding to describe the distribution of cancer cells and to explore the functional state of ZNF671 in cancer subgroups. We found that ZNF671 was downregulated in eight cancer-related ZNF671 scRNA-seq datasets. Functional analysis identified that ZNF671 might play a tumor suppressor role in cancer. The heterogeneous functional states of cell subgroups and correlation analysis showed that ZNF671 played tumor suppressor roles in heterogeneous cancer cell populations. Western blot and transwell assays identified that ZNF671 inhibited EMT, migration, and invasion of CNS cancers, lung cancer, melanoma, and breast carcinoma in vitro. These results from cancer single-cell sequencing indicated that ZNF671 played a tumor suppressor role in multiple tumors and may provide us with new insights into the role of ZNF671 for cancer treatment.
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spelling pubmed-68576222019-11-28 The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing Zhang, Jian Luo, Jianli Jiang, Huali Xie, Tao Zheng, Jieling Tian, Yunhong Li, Rong Wang, Baiyao Lin, Jie Xu, Anan Huang, Xiaoting Yuan, Yawei Front Oncol Oncology In humans, zinc finger protein 671 (ZNF671) is a type of transcription factor. However, the contribution of tumor heterogeneity to the functional role of ZNF671 remains unknown. The present study aimed to determine the functional states of ZNF671 in cancer single cells based on single-cell sequencing datasets (scRNA-seq). We collected cancer-related ZNF671 scRNA-seq datasets and analyzed ZNF671 in the datasets. We evaluated 14 functional states of ZNF671 in cancers and performed ZNF671 expression and function state correlation analysis. We further applied t-distributed stochastic neighbor embedding to describe the distribution of cancer cells and to explore the functional state of ZNF671 in cancer subgroups. We found that ZNF671 was downregulated in eight cancer-related ZNF671 scRNA-seq datasets. Functional analysis identified that ZNF671 might play a tumor suppressor role in cancer. The heterogeneous functional states of cell subgroups and correlation analysis showed that ZNF671 played tumor suppressor roles in heterogeneous cancer cell populations. Western blot and transwell assays identified that ZNF671 inhibited EMT, migration, and invasion of CNS cancers, lung cancer, melanoma, and breast carcinoma in vitro. These results from cancer single-cell sequencing indicated that ZNF671 played a tumor suppressor role in multiple tumors and may provide us with new insights into the role of ZNF671 for cancer treatment. Frontiers Media S.A. 2019-11-08 /pmc/articles/PMC6857622/ /pubmed/31781507 http://dx.doi.org/10.3389/fonc.2019.01214 Text en Copyright © 2019 Zhang, Luo, Jiang, Xie, Zheng, Tian, Li, Wang, Lin, Xu, Huang and Yuan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Zhang, Jian
Luo, Jianli
Jiang, Huali
Xie, Tao
Zheng, Jieling
Tian, Yunhong
Li, Rong
Wang, Baiyao
Lin, Jie
Xu, Anan
Huang, Xiaoting
Yuan, Yawei
The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing
title The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing
title_full The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing
title_fullStr The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing
title_full_unstemmed The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing
title_short The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing
title_sort tumor suppressor role of zinc finger protein 671 (znf671) in multiple tumors based on cancer single-cell sequencing
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857622/
https://www.ncbi.nlm.nih.gov/pubmed/31781507
http://dx.doi.org/10.3389/fonc.2019.01214
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