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Metabolic Dynamics of Developing Rice Seeds Under High Night-Time Temperature Stress
High temperature stress during rice reproductive development results in yield losses. Reduced grain yield and grain quality has been associated with high temperature stress, and specifically with high night-time temperatures (HNT). Characterizing the impact of HNT on the phenotypic and metabolic sta...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857699/ https://www.ncbi.nlm.nih.gov/pubmed/31781147 http://dx.doi.org/10.3389/fpls.2019.01443 |
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author | Dhatt, Balpreet K. Abshire, Nathan Paul, Puneet Hasanthika, Kalani Sandhu, Jaspreet Zhang, Qi Obata, Toshihiro Walia, Harkamal |
author_facet | Dhatt, Balpreet K. Abshire, Nathan Paul, Puneet Hasanthika, Kalani Sandhu, Jaspreet Zhang, Qi Obata, Toshihiro Walia, Harkamal |
author_sort | Dhatt, Balpreet K. |
collection | PubMed |
description | High temperature stress during rice reproductive development results in yield losses. Reduced grain yield and grain quality has been associated with high temperature stress, and specifically with high night-time temperatures (HNT). Characterizing the impact of HNT on the phenotypic and metabolic status of developing rice seeds can provide insights into the mechanisms involved in yield and quality decline. Here, we examined the impact of warmer nights on the morphology and metabolome during early seed development in six diverse rice accessions. Seed size was sensitive to HNT in four of the six genotypes, while seed fertility and seed weight were unaffected. We observed genotypic differences for negative impact of HNT on grain quality. This was evident from the chalky grain appearance due to impaired packaging of starch granules. Metabolite profiles during early seed development (3 and 4 days after fertilization; DAF) were distinct from the early grain filling stages (7 and 10 DAF) under optimal conditions. We observed that accumulation of sugars (sucrose, fructose, and glucose) peaked at 7 DAF suggesting a major flux of carbon into glycolysis, tricarboxylic acid cycle, and starch biosynthesis during grain filling. Next, we determined hyper (HNT > control) and hypo (HNT < control) abundant metabolites and found 19 of the 57 metabolites to differ significantly between HNT and control treatments. The most prominent changes were exhibited by differential abundance of sugar and sugar alcohols under HNT, which could be linked to a protective mechanism against the HNT damage. Overall, our results indicate that combining metabolic profiles of developing grains with yield and quality parameters under high night temperature stress could provide insight for exploration of natural variation for HNT tolerance in the rice germplasm. |
format | Online Article Text |
id | pubmed-6857699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68576992019-11-28 Metabolic Dynamics of Developing Rice Seeds Under High Night-Time Temperature Stress Dhatt, Balpreet K. Abshire, Nathan Paul, Puneet Hasanthika, Kalani Sandhu, Jaspreet Zhang, Qi Obata, Toshihiro Walia, Harkamal Front Plant Sci Plant Science High temperature stress during rice reproductive development results in yield losses. Reduced grain yield and grain quality has been associated with high temperature stress, and specifically with high night-time temperatures (HNT). Characterizing the impact of HNT on the phenotypic and metabolic status of developing rice seeds can provide insights into the mechanisms involved in yield and quality decline. Here, we examined the impact of warmer nights on the morphology and metabolome during early seed development in six diverse rice accessions. Seed size was sensitive to HNT in four of the six genotypes, while seed fertility and seed weight were unaffected. We observed genotypic differences for negative impact of HNT on grain quality. This was evident from the chalky grain appearance due to impaired packaging of starch granules. Metabolite profiles during early seed development (3 and 4 days after fertilization; DAF) were distinct from the early grain filling stages (7 and 10 DAF) under optimal conditions. We observed that accumulation of sugars (sucrose, fructose, and glucose) peaked at 7 DAF suggesting a major flux of carbon into glycolysis, tricarboxylic acid cycle, and starch biosynthesis during grain filling. Next, we determined hyper (HNT > control) and hypo (HNT < control) abundant metabolites and found 19 of the 57 metabolites to differ significantly between HNT and control treatments. The most prominent changes were exhibited by differential abundance of sugar and sugar alcohols under HNT, which could be linked to a protective mechanism against the HNT damage. Overall, our results indicate that combining metabolic profiles of developing grains with yield and quality parameters under high night temperature stress could provide insight for exploration of natural variation for HNT tolerance in the rice germplasm. Frontiers Media S.A. 2019-11-08 /pmc/articles/PMC6857699/ /pubmed/31781147 http://dx.doi.org/10.3389/fpls.2019.01443 Text en Copyright © 2019 Dhatt, Abshire, Paul, Hasanthika, Sandhu, Zhang, Obata and Walia http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Dhatt, Balpreet K. Abshire, Nathan Paul, Puneet Hasanthika, Kalani Sandhu, Jaspreet Zhang, Qi Obata, Toshihiro Walia, Harkamal Metabolic Dynamics of Developing Rice Seeds Under High Night-Time Temperature Stress |
title | Metabolic Dynamics of Developing Rice Seeds Under High Night-Time Temperature Stress |
title_full | Metabolic Dynamics of Developing Rice Seeds Under High Night-Time Temperature Stress |
title_fullStr | Metabolic Dynamics of Developing Rice Seeds Under High Night-Time Temperature Stress |
title_full_unstemmed | Metabolic Dynamics of Developing Rice Seeds Under High Night-Time Temperature Stress |
title_short | Metabolic Dynamics of Developing Rice Seeds Under High Night-Time Temperature Stress |
title_sort | metabolic dynamics of developing rice seeds under high night-time temperature stress |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857699/ https://www.ncbi.nlm.nih.gov/pubmed/31781147 http://dx.doi.org/10.3389/fpls.2019.01443 |
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