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Targeting the BRAF Signaling Pathway in CD133(pos) Cancer Stem Cells of Anaplastic Thyroid Carcinoma

BACKGROUND: Cancer stem cells (CSCs) with a self-renewal ability in tumor cells population, execute a pivotal function in tumorigenesis, retrogression, and metastasis of malignant cancers such as anaplastic thyroid carcinoma (ATC). MATERIALS AND METHODS: In this study, we isolated CSCs subpopulation...

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Autores principales: Bozorg-Ghalati, Farzaneh, Hedayati, Mehdi, Dianatpour, Mehdi, Mosaffa, Nariman, Azizi, Fereidoun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857869/
https://www.ncbi.nlm.nih.gov/pubmed/31127889
http://dx.doi.org/10.31557/APJCP.2019.20.5.1353
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author Bozorg-Ghalati, Farzaneh
Hedayati, Mehdi
Dianatpour, Mehdi
Mosaffa, Nariman
Azizi, Fereidoun
author_facet Bozorg-Ghalati, Farzaneh
Hedayati, Mehdi
Dianatpour, Mehdi
Mosaffa, Nariman
Azizi, Fereidoun
author_sort Bozorg-Ghalati, Farzaneh
collection PubMed
description BACKGROUND: Cancer stem cells (CSCs) with a self-renewal ability in tumor cells population, execute a pivotal function in tumorigenesis, retrogression, and metastasis of malignant cancers such as anaplastic thyroid carcinoma (ATC). MATERIALS AND METHODS: In this study, we isolated CSCs subpopulation with CD133 surface marker from three ATC cell lines by magnetic cell sorting assay. After confirming the segregation by the flow cytometry method, BRAF and sodium-iodide symporter (NIS) genes were investigated in them before and after incubation with BRAF inhibitor. Also, we evaluated the NIS protein expression and localization. RESULTS: Established upon q-RT PCR data, when compared to human normal thyrocytes, the BRAF(V600E) gene was over-expressed in CD133(pos) cells (>1705.99 ± 55.55 fold, Mean ± SEM, n=3, P- value<0.05), whilst the expression of NIS gene was very restricted (< 0.0008 ± 5.43 fold, Mean ± SEM, n=3, P- value<0.05) in them. Also, our results showed that BRAF inhibition affected NIS protein expression and localization. CONCLUSIONS: Current study showed that the differentiate genes/proteins expression can be induced in the CSCs via focus on signal transduction pathways and targeting their molecules, that are involved in expression of these genes/proteins. Therefore, attention to targeting CSCs along with routine thyroid cancer therapy, can help to ATC treatment.
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spelling pubmed-68578692019-12-12 Targeting the BRAF Signaling Pathway in CD133(pos) Cancer Stem Cells of Anaplastic Thyroid Carcinoma Bozorg-Ghalati, Farzaneh Hedayati, Mehdi Dianatpour, Mehdi Mosaffa, Nariman Azizi, Fereidoun Asian Pac J Cancer Prev Research Article BACKGROUND: Cancer stem cells (CSCs) with a self-renewal ability in tumor cells population, execute a pivotal function in tumorigenesis, retrogression, and metastasis of malignant cancers such as anaplastic thyroid carcinoma (ATC). MATERIALS AND METHODS: In this study, we isolated CSCs subpopulation with CD133 surface marker from three ATC cell lines by magnetic cell sorting assay. After confirming the segregation by the flow cytometry method, BRAF and sodium-iodide symporter (NIS) genes were investigated in them before and after incubation with BRAF inhibitor. Also, we evaluated the NIS protein expression and localization. RESULTS: Established upon q-RT PCR data, when compared to human normal thyrocytes, the BRAF(V600E) gene was over-expressed in CD133(pos) cells (>1705.99 ± 55.55 fold, Mean ± SEM, n=3, P- value<0.05), whilst the expression of NIS gene was very restricted (< 0.0008 ± 5.43 fold, Mean ± SEM, n=3, P- value<0.05) in them. Also, our results showed that BRAF inhibition affected NIS protein expression and localization. CONCLUSIONS: Current study showed that the differentiate genes/proteins expression can be induced in the CSCs via focus on signal transduction pathways and targeting their molecules, that are involved in expression of these genes/proteins. Therefore, attention to targeting CSCs along with routine thyroid cancer therapy, can help to ATC treatment. West Asia Organization for Cancer Prevention 2019 /pmc/articles/PMC6857869/ /pubmed/31127889 http://dx.doi.org/10.31557/APJCP.2019.20.5.1353 Text en Copyright: © Asian Pacific Journal of Cancer Prevention http://creativecommons.org/licenses/BY-SA/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Research Article
Bozorg-Ghalati, Farzaneh
Hedayati, Mehdi
Dianatpour, Mehdi
Mosaffa, Nariman
Azizi, Fereidoun
Targeting the BRAF Signaling Pathway in CD133(pos) Cancer Stem Cells of Anaplastic Thyroid Carcinoma
title Targeting the BRAF Signaling Pathway in CD133(pos) Cancer Stem Cells of Anaplastic Thyroid Carcinoma
title_full Targeting the BRAF Signaling Pathway in CD133(pos) Cancer Stem Cells of Anaplastic Thyroid Carcinoma
title_fullStr Targeting the BRAF Signaling Pathway in CD133(pos) Cancer Stem Cells of Anaplastic Thyroid Carcinoma
title_full_unstemmed Targeting the BRAF Signaling Pathway in CD133(pos) Cancer Stem Cells of Anaplastic Thyroid Carcinoma
title_short Targeting the BRAF Signaling Pathway in CD133(pos) Cancer Stem Cells of Anaplastic Thyroid Carcinoma
title_sort targeting the braf signaling pathway in cd133(pos) cancer stem cells of anaplastic thyroid carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857869/
https://www.ncbi.nlm.nih.gov/pubmed/31127889
http://dx.doi.org/10.31557/APJCP.2019.20.5.1353
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