Cargando…
Virus-borne mini-CRISPR arrays are involved in interviral conflicts
CRISPR-Cas immunity is at the forefront of antivirus defense in bacteria and archaea and specifically targets viruses carrying protospacers matching the spacers catalogued in the CRISPR arrays. Here, we perform deep sequencing of the CRISPRome—all spacers contained in a microbiome—associated with hy...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6858448/ https://www.ncbi.nlm.nih.gov/pubmed/31729390 http://dx.doi.org/10.1038/s41467-019-13205-2 |
_version_ | 1783470963182010368 |
---|---|
author | Medvedeva, Sofia Liu, Ying Koonin, Eugene V. Severinov, Konstantin Prangishvili, David Krupovic, Mart |
author_facet | Medvedeva, Sofia Liu, Ying Koonin, Eugene V. Severinov, Konstantin Prangishvili, David Krupovic, Mart |
author_sort | Medvedeva, Sofia |
collection | PubMed |
description | CRISPR-Cas immunity is at the forefront of antivirus defense in bacteria and archaea and specifically targets viruses carrying protospacers matching the spacers catalogued in the CRISPR arrays. Here, we perform deep sequencing of the CRISPRome—all spacers contained in a microbiome—associated with hyperthermophilic archaea of the order Sulfolobales recovered directly from an environmental sample and from enrichment cultures established in the laboratory. The 25 million CRISPR spacers sequenced from a single sampling site dwarf the diversity of spacers from all available Sulfolobales isolates and display complex temporal dynamics. Comparison of closely related virus strains shows that CRISPR targeting drives virus genome evolution. Furthermore, we show that some archaeal viruses carry mini-CRISPR arrays with 1–2 spacers and preceded by leader sequences but devoid of cas genes. Closely related viruses present in the same population carry spacers against each other. Targeting by these virus-borne spacers represents a distinct mechanism of heterotypic superinfection exclusion and appears to promote archaeal virus speciation. |
format | Online Article Text |
id | pubmed-6858448 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-68584482019-11-20 Virus-borne mini-CRISPR arrays are involved in interviral conflicts Medvedeva, Sofia Liu, Ying Koonin, Eugene V. Severinov, Konstantin Prangishvili, David Krupovic, Mart Nat Commun Article CRISPR-Cas immunity is at the forefront of antivirus defense in bacteria and archaea and specifically targets viruses carrying protospacers matching the spacers catalogued in the CRISPR arrays. Here, we perform deep sequencing of the CRISPRome—all spacers contained in a microbiome—associated with hyperthermophilic archaea of the order Sulfolobales recovered directly from an environmental sample and from enrichment cultures established in the laboratory. The 25 million CRISPR spacers sequenced from a single sampling site dwarf the diversity of spacers from all available Sulfolobales isolates and display complex temporal dynamics. Comparison of closely related virus strains shows that CRISPR targeting drives virus genome evolution. Furthermore, we show that some archaeal viruses carry mini-CRISPR arrays with 1–2 spacers and preceded by leader sequences but devoid of cas genes. Closely related viruses present in the same population carry spacers against each other. Targeting by these virus-borne spacers represents a distinct mechanism of heterotypic superinfection exclusion and appears to promote archaeal virus speciation. Nature Publishing Group UK 2019-11-15 /pmc/articles/PMC6858448/ /pubmed/31729390 http://dx.doi.org/10.1038/s41467-019-13205-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Medvedeva, Sofia Liu, Ying Koonin, Eugene V. Severinov, Konstantin Prangishvili, David Krupovic, Mart Virus-borne mini-CRISPR arrays are involved in interviral conflicts |
title | Virus-borne mini-CRISPR arrays are involved in interviral conflicts |
title_full | Virus-borne mini-CRISPR arrays are involved in interviral conflicts |
title_fullStr | Virus-borne mini-CRISPR arrays are involved in interviral conflicts |
title_full_unstemmed | Virus-borne mini-CRISPR arrays are involved in interviral conflicts |
title_short | Virus-borne mini-CRISPR arrays are involved in interviral conflicts |
title_sort | virus-borne mini-crispr arrays are involved in interviral conflicts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6858448/ https://www.ncbi.nlm.nih.gov/pubmed/31729390 http://dx.doi.org/10.1038/s41467-019-13205-2 |
work_keys_str_mv | AT medvedevasofia virusborneminicrisprarraysareinvolvedininterviralconflicts AT liuying virusborneminicrisprarraysareinvolvedininterviralconflicts AT koonineugenev virusborneminicrisprarraysareinvolvedininterviralconflicts AT severinovkonstantin virusborneminicrisprarraysareinvolvedininterviralconflicts AT prangishvilidavid virusborneminicrisprarraysareinvolvedininterviralconflicts AT krupovicmart virusborneminicrisprarraysareinvolvedininterviralconflicts |