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An efficient and comprehensive plant glycerolipids analysis approach based on high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometer

In past two decades, numerous lipidomics approaches based on mass spectrometry with or without liquid chromatography separation have been established for identification and quantification of lipids in plants. In this study, we developed an efficient and comprehensive lipidomics approach based on UPL...

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Detalles Bibliográficos
Autores principales: Lu, Shaoping, Liu, Hongbo, Jin, Cheng, Li, Qing, Guo, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6858605/
https://www.ncbi.nlm.nih.gov/pubmed/31832598
http://dx.doi.org/10.1002/pld3.183
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author Lu, Shaoping
Liu, Hongbo
Jin, Cheng
Li, Qing
Guo, Liang
author_facet Lu, Shaoping
Liu, Hongbo
Jin, Cheng
Li, Qing
Guo, Liang
author_sort Lu, Shaoping
collection PubMed
description In past two decades, numerous lipidomics approaches based on mass spectrometry with or without liquid chromatography separation have been established for identification and quantification of lipids in plants. In this study, we developed an efficient and comprehensive lipidomics approach based on UPLC with an Acquity UPLC(TM) BEH C18 column coupled to TripleTOF using ESI in positive ion mode and MS/MS(ALL) scan for data collection. Lipid extract was prepared to 2 mg/ml solution according to dry tissue weight and mixed with 13 kinds of internal standards including PA, PC, PE, and PG. Each analysis required single injection of 5–10 μl lipid solvent and completed in 32 min. A target method dataset was generated using the LipidView software for prediction of the accurate mass of target lipid species. The dataset was uploaded into the PeakView to create processing datasets to search target lipid species, which achieved batch data processing of multiple samples for lipid species‐specific identification and quantification. As proof of concept, we profiled the lipids of different tissues of rapeseed. Thirteen lipid classes including 218 glycerolipids were identified including 46 TAGs, 15 DAGs, 20 PCs, 24 PEs, 13 PGs, 14 PIs, 26 PSs, 12 PAs, 16 MGDGs, 16 DGDGs, 6 LysoPCs, 5 LysoPEs, and 5 LysoPGs. Together, our approach permits the analysis of glycerolipids in plant tissues with simplicity in sample analysis and data processing using UPLC‐TripleTOF.
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spelling pubmed-68586052019-12-12 An efficient and comprehensive plant glycerolipids analysis approach based on high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometer Lu, Shaoping Liu, Hongbo Jin, Cheng Li, Qing Guo, Liang Plant Direct Original Research In past two decades, numerous lipidomics approaches based on mass spectrometry with or without liquid chromatography separation have been established for identification and quantification of lipids in plants. In this study, we developed an efficient and comprehensive lipidomics approach based on UPLC with an Acquity UPLC(TM) BEH C18 column coupled to TripleTOF using ESI in positive ion mode and MS/MS(ALL) scan for data collection. Lipid extract was prepared to 2 mg/ml solution according to dry tissue weight and mixed with 13 kinds of internal standards including PA, PC, PE, and PG. Each analysis required single injection of 5–10 μl lipid solvent and completed in 32 min. A target method dataset was generated using the LipidView software for prediction of the accurate mass of target lipid species. The dataset was uploaded into the PeakView to create processing datasets to search target lipid species, which achieved batch data processing of multiple samples for lipid species‐specific identification and quantification. As proof of concept, we profiled the lipids of different tissues of rapeseed. Thirteen lipid classes including 218 glycerolipids were identified including 46 TAGs, 15 DAGs, 20 PCs, 24 PEs, 13 PGs, 14 PIs, 26 PSs, 12 PAs, 16 MGDGs, 16 DGDGs, 6 LysoPCs, 5 LysoPEs, and 5 LysoPGs. Together, our approach permits the analysis of glycerolipids in plant tissues with simplicity in sample analysis and data processing using UPLC‐TripleTOF. John Wiley and Sons Inc. 2019-11-15 /pmc/articles/PMC6858605/ /pubmed/31832598 http://dx.doi.org/10.1002/pld3.183 Text en © 2019 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Lu, Shaoping
Liu, Hongbo
Jin, Cheng
Li, Qing
Guo, Liang
An efficient and comprehensive plant glycerolipids analysis approach based on high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometer
title An efficient and comprehensive plant glycerolipids analysis approach based on high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometer
title_full An efficient and comprehensive plant glycerolipids analysis approach based on high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometer
title_fullStr An efficient and comprehensive plant glycerolipids analysis approach based on high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometer
title_full_unstemmed An efficient and comprehensive plant glycerolipids analysis approach based on high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometer
title_short An efficient and comprehensive plant glycerolipids analysis approach based on high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometer
title_sort efficient and comprehensive plant glycerolipids analysis approach based on high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6858605/
https://www.ncbi.nlm.nih.gov/pubmed/31832598
http://dx.doi.org/10.1002/pld3.183
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