Substantially improving the enantioconvergence of PvEH1, a Phaseolus vulgaris epoxide hydrolase, towards m-chlorostyrene oxide by laboratory evolution

BACKGROUND: Epoxide hydrolase can regioselectively catalyze the oxirane ring-opening hydrolysis of rac-epoxides producing the corresponding chiral diols. In our laboratory, a gene named pveh1 encoding an EH from Phaseolus vulgaris was cloned. Although the directed modification of PvEH1 was carried o...

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Detalles Bibliográficos
Autores principales: Zong, Xun-Cheng, Li, Chuang, Xu, Yao-Hui, Hu, Die, Hu, Bo-Chun, Zang, Jia, Wu, Min-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859628/
https://www.ncbi.nlm.nih.gov/pubmed/31739786
http://dx.doi.org/10.1186/s12934-019-1252-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859628/
https://www.ncbi.nlm.nih.gov/pubmed/31739786
http://dx.doi.org/10.1186/s12934-019-1252-4

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