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Duplication history and molecular evolution of the rbcS multigene family in angiosperms
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is considered to be the main enzyme determining the rate of photosynthesis. The small subunit of the protein, encoded by the rbcS gene, has been shown to influence the catalytic efficiency, CO(2) specificity, assembly, activity, and stability...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859733/ https://www.ncbi.nlm.nih.gov/pubmed/31498865 http://dx.doi.org/10.1093/jxb/erz363 |
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author | Yamada, Kana Davydov, Iakov I Besnard, Guillaume Salamin, Nicolas |
author_facet | Yamada, Kana Davydov, Iakov I Besnard, Guillaume Salamin, Nicolas |
author_sort | Yamada, Kana |
collection | PubMed |
description | Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is considered to be the main enzyme determining the rate of photosynthesis. The small subunit of the protein, encoded by the rbcS gene, has been shown to influence the catalytic efficiency, CO(2) specificity, assembly, activity, and stability of RuBisCO. However, the evolution of the rbcS gene remains poorly studied. We inferred the phylogenetic tree of the rbcS gene in angiosperms using the nucleotide sequences and found that it is composed of two lineages that may have existed before the divergence of land plants. Although almost all species sampled carry at least one copy of lineage 1, genes of lineage 2 were lost in most angiosperm species. We found the specific residues that have undergone positive selection during the evolution of the rbcS gene. We detected intensive coevolution between each rbcS gene copy and the rbcL gene encoding the large subunit of RuBisCO. We tested the role played by each rbcS gene copy on the stability of the RuBisCO protein through homology modelling. Our results showed that this evolutionary constraint could limit the level of divergence seen in the rbcS gene, which leads to the similarity among the rbcS gene copies of lineage 1 within species. |
format | Online Article Text |
id | pubmed-6859733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-68597332019-11-21 Duplication history and molecular evolution of the rbcS multigene family in angiosperms Yamada, Kana Davydov, Iakov I Besnard, Guillaume Salamin, Nicolas J Exp Bot Research Papers Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is considered to be the main enzyme determining the rate of photosynthesis. The small subunit of the protein, encoded by the rbcS gene, has been shown to influence the catalytic efficiency, CO(2) specificity, assembly, activity, and stability of RuBisCO. However, the evolution of the rbcS gene remains poorly studied. We inferred the phylogenetic tree of the rbcS gene in angiosperms using the nucleotide sequences and found that it is composed of two lineages that may have existed before the divergence of land plants. Although almost all species sampled carry at least one copy of lineage 1, genes of lineage 2 were lost in most angiosperm species. We found the specific residues that have undergone positive selection during the evolution of the rbcS gene. We detected intensive coevolution between each rbcS gene copy and the rbcL gene encoding the large subunit of RuBisCO. We tested the role played by each rbcS gene copy on the stability of the RuBisCO protein through homology modelling. Our results showed that this evolutionary constraint could limit the level of divergence seen in the rbcS gene, which leads to the similarity among the rbcS gene copies of lineage 1 within species. Oxford University Press 2019-11-01 2019-09-09 /pmc/articles/PMC6859733/ /pubmed/31498865 http://dx.doi.org/10.1093/jxb/erz363 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Papers Yamada, Kana Davydov, Iakov I Besnard, Guillaume Salamin, Nicolas Duplication history and molecular evolution of the rbcS multigene family in angiosperms |
title | Duplication history and molecular evolution of the rbcS multigene family in angiosperms |
title_full | Duplication history and molecular evolution of the rbcS multigene family in angiosperms |
title_fullStr | Duplication history and molecular evolution of the rbcS multigene family in angiosperms |
title_full_unstemmed | Duplication history and molecular evolution of the rbcS multigene family in angiosperms |
title_short | Duplication history and molecular evolution of the rbcS multigene family in angiosperms |
title_sort | duplication history and molecular evolution of the rbcs multigene family in angiosperms |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859733/ https://www.ncbi.nlm.nih.gov/pubmed/31498865 http://dx.doi.org/10.1093/jxb/erz363 |
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