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Ultrastructural study of adult Haemonchus contortus exposed to polyphenol-rich materials under in vivo conditions in goats

This study assessed the ultrastructural changes caused in adult Haemonchus contortus obtained from goats fed fodder based on polyphenol-rich plants Lysiloma latisiliquum or Onobrychis viciifolia or from goats drenched with quebracho extract, Schinopsis spp. The H. contortus were obtained from artifi...

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Autores principales: Martínez-Ortiz-de-Montellano, Cintli, Torres-Acosta, Juan Felipe de Jesús, Fourquaux, Isabelle, Sandoval-Castro, Carlos Alfredo, Hoste, Hervé
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859865/
https://www.ncbi.nlm.nih.gov/pubmed/31738160
http://dx.doi.org/10.1051/parasite/2019065
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author Martínez-Ortiz-de-Montellano, Cintli
Torres-Acosta, Juan Felipe de Jesús
Fourquaux, Isabelle
Sandoval-Castro, Carlos Alfredo
Hoste, Hervé
author_facet Martínez-Ortiz-de-Montellano, Cintli
Torres-Acosta, Juan Felipe de Jesús
Fourquaux, Isabelle
Sandoval-Castro, Carlos Alfredo
Hoste, Hervé
author_sort Martínez-Ortiz-de-Montellano, Cintli
collection PubMed
description This study assessed the ultrastructural changes caused in adult Haemonchus contortus obtained from goats fed fodder based on polyphenol-rich plants Lysiloma latisiliquum or Onobrychis viciifolia or from goats drenched with quebracho extract, Schinopsis spp. The H. contortus were obtained from artificially infected goats used as models to investigate the anthelmintic effect of feeding or drenching with the polyphenol-rich materials. Nematode populations were exposed to polyphenol-rich plant materials by feeding host goats for 8 consecutive days (D28 to D35 post-infection) with (a) L. latisiliquum fodder at 800 g fresh basis/day, (b) O. viciifolia fodder offered ad libitum, and (c) drenched with a solution containing quebracho extract (90 g/day). Meanwhile, control H. contortus were obtained from goats fed polyphenol-free diets. The H. contortus specimens were recovered from the goats on D36 post-infection, and transmission electron microscopy (TEM) was used to identify ultrastructural changes. In vivo exposure to different polyphenol-rich plant materials caused vacuolization of the nematodes’ intestinal, muscular and hypodermal cells. These alterations represent the first evidence of cell damage caused in H. contortus when hosts were fed or drenched with polyphenol-rich materials. Ultrastructural changes affecting several types of cells could explain modifications in worm motility and nutrition, eventually affecting H. contortus reproductive success. This study contributes to our understanding of the mechanisms of action of polyphenol-rich plants against H. contortus when given as nutraceuticals to goats.
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spelling pubmed-68598652019-12-05 Ultrastructural study of adult Haemonchus contortus exposed to polyphenol-rich materials under in vivo conditions in goats Martínez-Ortiz-de-Montellano, Cintli Torres-Acosta, Juan Felipe de Jesús Fourquaux, Isabelle Sandoval-Castro, Carlos Alfredo Hoste, Hervé Parasite Research Article This study assessed the ultrastructural changes caused in adult Haemonchus contortus obtained from goats fed fodder based on polyphenol-rich plants Lysiloma latisiliquum or Onobrychis viciifolia or from goats drenched with quebracho extract, Schinopsis spp. The H. contortus were obtained from artificially infected goats used as models to investigate the anthelmintic effect of feeding or drenching with the polyphenol-rich materials. Nematode populations were exposed to polyphenol-rich plant materials by feeding host goats for 8 consecutive days (D28 to D35 post-infection) with (a) L. latisiliquum fodder at 800 g fresh basis/day, (b) O. viciifolia fodder offered ad libitum, and (c) drenched with a solution containing quebracho extract (90 g/day). Meanwhile, control H. contortus were obtained from goats fed polyphenol-free diets. The H. contortus specimens were recovered from the goats on D36 post-infection, and transmission electron microscopy (TEM) was used to identify ultrastructural changes. In vivo exposure to different polyphenol-rich plant materials caused vacuolization of the nematodes’ intestinal, muscular and hypodermal cells. These alterations represent the first evidence of cell damage caused in H. contortus when hosts were fed or drenched with polyphenol-rich materials. Ultrastructural changes affecting several types of cells could explain modifications in worm motility and nutrition, eventually affecting H. contortus reproductive success. This study contributes to our understanding of the mechanisms of action of polyphenol-rich plants against H. contortus when given as nutraceuticals to goats. EDP Sciences 2019-11-18 /pmc/articles/PMC6859865/ /pubmed/31738160 http://dx.doi.org/10.1051/parasite/2019065 Text en © C. Martínez-Ortiz-de-Montellano et al., published by EDP Sciences, 2019 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Martínez-Ortiz-de-Montellano, Cintli
Torres-Acosta, Juan Felipe de Jesús
Fourquaux, Isabelle
Sandoval-Castro, Carlos Alfredo
Hoste, Hervé
Ultrastructural study of adult Haemonchus contortus exposed to polyphenol-rich materials under in vivo conditions in goats
title Ultrastructural study of adult Haemonchus contortus exposed to polyphenol-rich materials under in vivo conditions in goats
title_full Ultrastructural study of adult Haemonchus contortus exposed to polyphenol-rich materials under in vivo conditions in goats
title_fullStr Ultrastructural study of adult Haemonchus contortus exposed to polyphenol-rich materials under in vivo conditions in goats
title_full_unstemmed Ultrastructural study of adult Haemonchus contortus exposed to polyphenol-rich materials under in vivo conditions in goats
title_short Ultrastructural study of adult Haemonchus contortus exposed to polyphenol-rich materials under in vivo conditions in goats
title_sort ultrastructural study of adult haemonchus contortus exposed to polyphenol-rich materials under in vivo conditions in goats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859865/
https://www.ncbi.nlm.nih.gov/pubmed/31738160
http://dx.doi.org/10.1051/parasite/2019065
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