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Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass
BACKGROUND: Ovarian cancer is the fifth most common cancer in women worldwide. Moreover, there are no reliable minimal invasive tests to secure the diagnosis of malignant pelvic masses. Cell-free, circulating microRNAs have the potential as diagnostic biomarkers in cancer. Here, we performed and val...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6860451/ https://www.ncbi.nlm.nih.gov/pubmed/31738788 http://dx.doi.org/10.1371/journal.pone.0225249 |
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author | Oliveira, Douglas Nogueira Perez Carlsen, Anting Liu Heegaard, Niels H. H. Prahm, Kira Philipsen Christensen, Ib Jarle Høgdall, Claus K. Høgdall, Estrid V. |
author_facet | Oliveira, Douglas Nogueira Perez Carlsen, Anting Liu Heegaard, Niels H. H. Prahm, Kira Philipsen Christensen, Ib Jarle Høgdall, Claus K. Høgdall, Estrid V. |
author_sort | Oliveira, Douglas Nogueira Perez |
collection | PubMed |
description | BACKGROUND: Ovarian cancer is the fifth most common cancer in women worldwide. Moreover, there are no reliable minimal invasive tests to secure the diagnosis of malignant pelvic masses. Cell-free, circulating microRNAs have the potential as diagnostic biomarkers in cancer. Here, we performed and validated a miRNA panel with the potential to distinguish OC from benign pelvic masses. METHODS: The profile of plasma microRNA was determined with a panel of 46 candidates in a discovery group and a validation group, each consisting of 190 pre-surgery plasma samples from age-matched patients with malignant (n = 95) and benign pelvic mass (n = 95), by real time RT-qPCR. RESULTS: Four up-regulated (miR-200c-3p, miR-221-3p, miR-21-5p, and miR-484) and two down-regulated (miR-195-5p and miR-451a) microRNAs were discovered. From those, miR-200c-3p and miR-221-3p were further confirmed in a validation cohort. A combination of these 2 microRNAs together with CA-125 yielded an overall diagnostic accuracy of AUC = 0.96. CONCLUSIONS: We showed consistent plasma microRNA profiles that provide independent diagnostic information of late stage OC. |
format | Online Article Text |
id | pubmed-6860451 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-68604512019-12-07 Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass Oliveira, Douglas Nogueira Perez Carlsen, Anting Liu Heegaard, Niels H. H. Prahm, Kira Philipsen Christensen, Ib Jarle Høgdall, Claus K. Høgdall, Estrid V. PLoS One Research Article BACKGROUND: Ovarian cancer is the fifth most common cancer in women worldwide. Moreover, there are no reliable minimal invasive tests to secure the diagnosis of malignant pelvic masses. Cell-free, circulating microRNAs have the potential as diagnostic biomarkers in cancer. Here, we performed and validated a miRNA panel with the potential to distinguish OC from benign pelvic masses. METHODS: The profile of plasma microRNA was determined with a panel of 46 candidates in a discovery group and a validation group, each consisting of 190 pre-surgery plasma samples from age-matched patients with malignant (n = 95) and benign pelvic mass (n = 95), by real time RT-qPCR. RESULTS: Four up-regulated (miR-200c-3p, miR-221-3p, miR-21-5p, and miR-484) and two down-regulated (miR-195-5p and miR-451a) microRNAs were discovered. From those, miR-200c-3p and miR-221-3p were further confirmed in a validation cohort. A combination of these 2 microRNAs together with CA-125 yielded an overall diagnostic accuracy of AUC = 0.96. CONCLUSIONS: We showed consistent plasma microRNA profiles that provide independent diagnostic information of late stage OC. Public Library of Science 2019-11-18 /pmc/articles/PMC6860451/ /pubmed/31738788 http://dx.doi.org/10.1371/journal.pone.0225249 Text en © 2019 Oliveira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Oliveira, Douglas Nogueira Perez Carlsen, Anting Liu Heegaard, Niels H. H. Prahm, Kira Philipsen Christensen, Ib Jarle Høgdall, Claus K. Høgdall, Estrid V. Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass |
title | Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass |
title_full | Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass |
title_fullStr | Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass |
title_full_unstemmed | Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass |
title_short | Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass |
title_sort | diagnostic plasma mirna-profiles for ovarian cancer in patients with pelvic mass |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6860451/ https://www.ncbi.nlm.nih.gov/pubmed/31738788 http://dx.doi.org/10.1371/journal.pone.0225249 |
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