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Disentangling the sites of non-photochemical quenching in vascular plants

In nature, plants experience large fluctuations in light intensity and they need to balance the absorption and utilization of this energy accordingly. Non-photochemical quenching (NPQ) is a rapidly-switchable mechanism which protects plants from photodamage caused by high light exposure by dissipati...

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Autores principales: Nicol, Lauren, Nawrocki, Wojciech J., Croce, Roberta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6861128/
https://www.ncbi.nlm.nih.gov/pubmed/31659240
http://dx.doi.org/10.1038/s41477-019-0526-5
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author Nicol, Lauren
Nawrocki, Wojciech J.
Croce, Roberta
author_facet Nicol, Lauren
Nawrocki, Wojciech J.
Croce, Roberta
author_sort Nicol, Lauren
collection PubMed
description In nature, plants experience large fluctuations in light intensity and they need to balance the absorption and utilization of this energy accordingly. Non-photochemical quenching (NPQ) is a rapidly-switchable mechanism which protects plants from photodamage caused by high light exposure by dissipating the energy absorbed in excess as heat. It is triggered by the ΔpH across the thylakoid membrane and requires the presence of the protein PsbS and the xanthophyll zeaxanthin. However, the site and mechanism of the quencher(s) remain equivocal. Here, we constructed a mutant of Arabidopsis thaliana which lacks LHCII, the main antenna complexes of plants, to verify its contribution to NPQ. The mutant has normally stacked thylakoid membranes, displays no upregulation of other LHCs but shows a relative decrease in PSI which compensates for the decrease of the PSII antenna. The mutant exhibits a ~60% reduction in NPQ, while the remaining NPQ resembles that of the Chl b-less mutant, which lacks all PSII peripheral antenna complexes. We thus report that PsbS-dependent NPQ mainly occurs within LHCII, but there is an additional quenching site within the PSII core.
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spelling pubmed-68611282020-04-28 Disentangling the sites of non-photochemical quenching in vascular plants Nicol, Lauren Nawrocki, Wojciech J. Croce, Roberta Nat Plants Article In nature, plants experience large fluctuations in light intensity and they need to balance the absorption and utilization of this energy accordingly. Non-photochemical quenching (NPQ) is a rapidly-switchable mechanism which protects plants from photodamage caused by high light exposure by dissipating the energy absorbed in excess as heat. It is triggered by the ΔpH across the thylakoid membrane and requires the presence of the protein PsbS and the xanthophyll zeaxanthin. However, the site and mechanism of the quencher(s) remain equivocal. Here, we constructed a mutant of Arabidopsis thaliana which lacks LHCII, the main antenna complexes of plants, to verify its contribution to NPQ. The mutant has normally stacked thylakoid membranes, displays no upregulation of other LHCs but shows a relative decrease in PSI which compensates for the decrease of the PSII antenna. The mutant exhibits a ~60% reduction in NPQ, while the remaining NPQ resembles that of the Chl b-less mutant, which lacks all PSII peripheral antenna complexes. We thus report that PsbS-dependent NPQ mainly occurs within LHCII, but there is an additional quenching site within the PSII core. 2019-10-28 2019-11 /pmc/articles/PMC6861128/ /pubmed/31659240 http://dx.doi.org/10.1038/s41477-019-0526-5 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Nicol, Lauren
Nawrocki, Wojciech J.
Croce, Roberta
Disentangling the sites of non-photochemical quenching in vascular plants
title Disentangling the sites of non-photochemical quenching in vascular plants
title_full Disentangling the sites of non-photochemical quenching in vascular plants
title_fullStr Disentangling the sites of non-photochemical quenching in vascular plants
title_full_unstemmed Disentangling the sites of non-photochemical quenching in vascular plants
title_short Disentangling the sites of non-photochemical quenching in vascular plants
title_sort disentangling the sites of non-photochemical quenching in vascular plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6861128/
https://www.ncbi.nlm.nih.gov/pubmed/31659240
http://dx.doi.org/10.1038/s41477-019-0526-5
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