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PES1 In Liver Cancer: A Prognostic Biomarker With Tumorigenic Roles

PURPOSE: Liver cancer has a high incidence of mortality. DNA replication and posttranscriptional modifications play important roles in the development of liver cancer. Pescadillo (PES1) is a nuclear protein that is involved in embryonic development, ribosome synthesis, DNA replication, and cell cycl...

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Autores principales: Fu, Zhuo, Jiao, Yan, Li, Yan-Qing, Ke, Jian-Ji, Xu, Yan-Hui, Jia, Bao-Xing, Liu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6861535/
https://www.ncbi.nlm.nih.gov/pubmed/31814761
http://dx.doi.org/10.2147/CMAR.S226471
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author Fu, Zhuo
Jiao, Yan
Li, Yan-Qing
Ke, Jian-Ji
Xu, Yan-Hui
Jia, Bao-Xing
Liu, Bin
author_facet Fu, Zhuo
Jiao, Yan
Li, Yan-Qing
Ke, Jian-Ji
Xu, Yan-Hui
Jia, Bao-Xing
Liu, Bin
author_sort Fu, Zhuo
collection PubMed
description PURPOSE: Liver cancer has a high incidence of mortality. DNA replication and posttranscriptional modifications play important roles in the development of liver cancer. Pescadillo (PES1) is a nuclear protein that is involved in embryonic development, ribosome synthesis, DNA replication, and cell cycle progression. Recently, abnormal PES1 expression was reported in several tumors, including neuroblastoma, colon cancer, gastric cancer, and breast cancer. Based on bio-informatic analysis, cell experiments and animal models, the aim of this study is to investigate the expression patterns and specific roles of PES1 in liver cancer. PATIENTS AND METHODS: PES1 expression was represented by boxplots. The correlation between PES1 expression and clinical features was assessed by the chi-squared test and Fisher’s exact tests. Kaplan–Meier curves compared overall survival between different levels of PES1 expression, and Cox analysis selected potential variables associated with overall survival. The MTT assay investigated the proliferation rate, the scratch assay assessed the migratory ability, and the Transwell assay evaluated the invasion capacity of tumor cells in vitro. Animal models were used to confirm the tumorigenic roles of PES1 in vivo. GSEA illustrated the molecular mechanisms that PES1 participated in. RESULTS: We found that PES1 was highly expressed in liver cancer tissues, served as a diagnostic marker, and correlated with poor overall survival (OS) and relapse-free survival (RFS) in patients. In vitro studies indicated that PES1 promoted tumor cell proliferation (P=0.0034), migration (P=0.0026), and invasion (P=0.0008), and this tumorigenic role was confirmed in animal models. GSEA further illuminated molecular mechanisms that PES1 participated in liver cancer occurrence and progression. CONCLUSION: This study suggested that PES1 was upregulated in liver cancer and correlated with poor prognosis, by promoting tumor cell proliferation, migration, and invasion, and PES1 may be a novel diagnostic and prognostic bio-marker and a promising therapeutic target in liver cancer.
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spelling pubmed-68615352019-12-06 PES1 In Liver Cancer: A Prognostic Biomarker With Tumorigenic Roles Fu, Zhuo Jiao, Yan Li, Yan-Qing Ke, Jian-Ji Xu, Yan-Hui Jia, Bao-Xing Liu, Bin Cancer Manag Res Original Research PURPOSE: Liver cancer has a high incidence of mortality. DNA replication and posttranscriptional modifications play important roles in the development of liver cancer. Pescadillo (PES1) is a nuclear protein that is involved in embryonic development, ribosome synthesis, DNA replication, and cell cycle progression. Recently, abnormal PES1 expression was reported in several tumors, including neuroblastoma, colon cancer, gastric cancer, and breast cancer. Based on bio-informatic analysis, cell experiments and animal models, the aim of this study is to investigate the expression patterns and specific roles of PES1 in liver cancer. PATIENTS AND METHODS: PES1 expression was represented by boxplots. The correlation between PES1 expression and clinical features was assessed by the chi-squared test and Fisher’s exact tests. Kaplan–Meier curves compared overall survival between different levels of PES1 expression, and Cox analysis selected potential variables associated with overall survival. The MTT assay investigated the proliferation rate, the scratch assay assessed the migratory ability, and the Transwell assay evaluated the invasion capacity of tumor cells in vitro. Animal models were used to confirm the tumorigenic roles of PES1 in vivo. GSEA illustrated the molecular mechanisms that PES1 participated in. RESULTS: We found that PES1 was highly expressed in liver cancer tissues, served as a diagnostic marker, and correlated with poor overall survival (OS) and relapse-free survival (RFS) in patients. In vitro studies indicated that PES1 promoted tumor cell proliferation (P=0.0034), migration (P=0.0026), and invasion (P=0.0008), and this tumorigenic role was confirmed in animal models. GSEA further illuminated molecular mechanisms that PES1 participated in liver cancer occurrence and progression. CONCLUSION: This study suggested that PES1 was upregulated in liver cancer and correlated with poor prognosis, by promoting tumor cell proliferation, migration, and invasion, and PES1 may be a novel diagnostic and prognostic bio-marker and a promising therapeutic target in liver cancer. Dove 2019-11-14 /pmc/articles/PMC6861535/ /pubmed/31814761 http://dx.doi.org/10.2147/CMAR.S226471 Text en © 2019 Fu et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Fu, Zhuo
Jiao, Yan
Li, Yan-Qing
Ke, Jian-Ji
Xu, Yan-Hui
Jia, Bao-Xing
Liu, Bin
PES1 In Liver Cancer: A Prognostic Biomarker With Tumorigenic Roles
title PES1 In Liver Cancer: A Prognostic Biomarker With Tumorigenic Roles
title_full PES1 In Liver Cancer: A Prognostic Biomarker With Tumorigenic Roles
title_fullStr PES1 In Liver Cancer: A Prognostic Biomarker With Tumorigenic Roles
title_full_unstemmed PES1 In Liver Cancer: A Prognostic Biomarker With Tumorigenic Roles
title_short PES1 In Liver Cancer: A Prognostic Biomarker With Tumorigenic Roles
title_sort pes1 in liver cancer: a prognostic biomarker with tumorigenic roles
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6861535/
https://www.ncbi.nlm.nih.gov/pubmed/31814761
http://dx.doi.org/10.2147/CMAR.S226471
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