Rapid, simplified microscale quantitative analysis of lignin H/G/S composition with GC–MS in glass ampules and glass capillaries

The characterization of monolignols H-, G- and S- units composition Vanholme et al. (2010) of lignin is important in agriculture, forestry, herb medicine, livestock, and health care research Vanholme et al. (2008) and Sticklen (2008). The conventional methods often require a great deal of samples and reagents and are time-consuming. Here, we present a newly developed method with fewer operations. The optimized method is suitable for detecting and characterizing lignin composition of cell wall in different plant species and has the advantages of: • Avoiding the influence of plasticizer by plasticware and enhancing the accuracy of monolignols analysis. • Lowering the required samples from grams to milligrams, and organic reagents from milliliters to microliters. • Reducing the time required from a few days to 6 h.