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Cytoprotective Effect of Antioxidant Pentapeptides from the Protein Hydrolysate of Swim Bladders of Miiuy Croaker (Miichthys miiuy) against H(2)O(2)-Mediated Human Umbilical Vein Endothelial Cell (HUVEC) Injury

In our previous research, ten antioxidant pentapeptides including FYKWP, FTGMD, GFEPY, YLPYA, FPPYERRQ, GFYAA, FSGLR, FPYLRH, VPDDD, and GIEWA were identified from the hydrolysate of miiuy croaker (Miichthys miiuy) swim bladder. In this work, their protective function on H(2)O(2)-induced oxidative d...

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Autores principales: Cai, Shi-Ying, Wang, Yu-Mei, Zhao, Yu-Qin, Chi, Chang-Feng, Wang, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862189/
https://www.ncbi.nlm.nih.gov/pubmed/31683554
http://dx.doi.org/10.3390/ijms20215425
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author Cai, Shi-Ying
Wang, Yu-Mei
Zhao, Yu-Qin
Chi, Chang-Feng
Wang, Bin
author_facet Cai, Shi-Ying
Wang, Yu-Mei
Zhao, Yu-Qin
Chi, Chang-Feng
Wang, Bin
author_sort Cai, Shi-Ying
collection PubMed
description In our previous research, ten antioxidant pentapeptides including FYKWP, FTGMD, GFEPY, YLPYA, FPPYERRQ, GFYAA, FSGLR, FPYLRH, VPDDD, and GIEWA were identified from the hydrolysate of miiuy croaker (Miichthys miiuy) swim bladder. In this work, their protective function on H(2)O(2)-induced oxidative damage to human umbilical vein endothelial cells (HUVECs) was studied. Results indicated that there was no significant difference in the HUVEC viability between the normal group and the treated groups with the 10 pentapeptides at the concentration of 100 μM for 24 h (p < 0.05). Furthermore, FPYLRH of 100 μg/mL extremely significantly (p < 0.001) increased the viability (80.58% ± 5.01%) of HUVECs with H(2)O(2)-induced oxidative damage compared with that of the model group. The protective mechanism indicated that FPYLRH could extremely significantly (p < 0.001) increase the levels of superoxide dismutase (SOD) (211.36 ± 8.29 U/mg prot) and GSH-Px (53.06 ± 2.34 U/mg prot) and decrease the contents of reactive oxygen species (ROS) (139.1 ± 11.8% of control), malondialdehyde (MDA) (13.66 ± 0.71 nM/mg), and nitric oxide (NO) (4.36 ± 0.32 µM/L) at the concentration of 100 μM in HUVECs with H(2)O(2)-induced oxidative damage compared with those of the model group. In addition, FPYLRH dose-dependently protected DNA in oxidative damage HUVECs model. These results suggested that FPYLRH could significantly attenuate the H(2)O(2)-induced stress injury in HUVECs and might be used as a potential natural antioxidant in the functional food industries.
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spelling pubmed-68621892019-12-05 Cytoprotective Effect of Antioxidant Pentapeptides from the Protein Hydrolysate of Swim Bladders of Miiuy Croaker (Miichthys miiuy) against H(2)O(2)-Mediated Human Umbilical Vein Endothelial Cell (HUVEC) Injury Cai, Shi-Ying Wang, Yu-Mei Zhao, Yu-Qin Chi, Chang-Feng Wang, Bin Int J Mol Sci Article In our previous research, ten antioxidant pentapeptides including FYKWP, FTGMD, GFEPY, YLPYA, FPPYERRQ, GFYAA, FSGLR, FPYLRH, VPDDD, and GIEWA were identified from the hydrolysate of miiuy croaker (Miichthys miiuy) swim bladder. In this work, their protective function on H(2)O(2)-induced oxidative damage to human umbilical vein endothelial cells (HUVECs) was studied. Results indicated that there was no significant difference in the HUVEC viability between the normal group and the treated groups with the 10 pentapeptides at the concentration of 100 μM for 24 h (p < 0.05). Furthermore, FPYLRH of 100 μg/mL extremely significantly (p < 0.001) increased the viability (80.58% ± 5.01%) of HUVECs with H(2)O(2)-induced oxidative damage compared with that of the model group. The protective mechanism indicated that FPYLRH could extremely significantly (p < 0.001) increase the levels of superoxide dismutase (SOD) (211.36 ± 8.29 U/mg prot) and GSH-Px (53.06 ± 2.34 U/mg prot) and decrease the contents of reactive oxygen species (ROS) (139.1 ± 11.8% of control), malondialdehyde (MDA) (13.66 ± 0.71 nM/mg), and nitric oxide (NO) (4.36 ± 0.32 µM/L) at the concentration of 100 μM in HUVECs with H(2)O(2)-induced oxidative damage compared with those of the model group. In addition, FPYLRH dose-dependently protected DNA in oxidative damage HUVECs model. These results suggested that FPYLRH could significantly attenuate the H(2)O(2)-induced stress injury in HUVECs and might be used as a potential natural antioxidant in the functional food industries. MDPI 2019-10-31 /pmc/articles/PMC6862189/ /pubmed/31683554 http://dx.doi.org/10.3390/ijms20215425 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cai, Shi-Ying
Wang, Yu-Mei
Zhao, Yu-Qin
Chi, Chang-Feng
Wang, Bin
Cytoprotective Effect of Antioxidant Pentapeptides from the Protein Hydrolysate of Swim Bladders of Miiuy Croaker (Miichthys miiuy) against H(2)O(2)-Mediated Human Umbilical Vein Endothelial Cell (HUVEC) Injury
title Cytoprotective Effect of Antioxidant Pentapeptides from the Protein Hydrolysate of Swim Bladders of Miiuy Croaker (Miichthys miiuy) against H(2)O(2)-Mediated Human Umbilical Vein Endothelial Cell (HUVEC) Injury
title_full Cytoprotective Effect of Antioxidant Pentapeptides from the Protein Hydrolysate of Swim Bladders of Miiuy Croaker (Miichthys miiuy) against H(2)O(2)-Mediated Human Umbilical Vein Endothelial Cell (HUVEC) Injury
title_fullStr Cytoprotective Effect of Antioxidant Pentapeptides from the Protein Hydrolysate of Swim Bladders of Miiuy Croaker (Miichthys miiuy) against H(2)O(2)-Mediated Human Umbilical Vein Endothelial Cell (HUVEC) Injury
title_full_unstemmed Cytoprotective Effect of Antioxidant Pentapeptides from the Protein Hydrolysate of Swim Bladders of Miiuy Croaker (Miichthys miiuy) against H(2)O(2)-Mediated Human Umbilical Vein Endothelial Cell (HUVEC) Injury
title_short Cytoprotective Effect of Antioxidant Pentapeptides from the Protein Hydrolysate of Swim Bladders of Miiuy Croaker (Miichthys miiuy) against H(2)O(2)-Mediated Human Umbilical Vein Endothelial Cell (HUVEC) Injury
title_sort cytoprotective effect of antioxidant pentapeptides from the protein hydrolysate of swim bladders of miiuy croaker (miichthys miiuy) against h(2)o(2)-mediated human umbilical vein endothelial cell (huvec) injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862189/
https://www.ncbi.nlm.nih.gov/pubmed/31683554
http://dx.doi.org/10.3390/ijms20215425
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