Cargando…
Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2 Arabidopsis thaliana Katanin Mutant
Meloidogyne incognita is a root knot nematode (RKN) species which is among the most notoriously unmanageable crop pests with a wide host range. It inhabits plants and induces unique feeding site structures within host roots, known as giant cells (GCs). The cell walls of the GCs undergo the process o...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862268/ https://www.ncbi.nlm.nih.gov/pubmed/31684028 http://dx.doi.org/10.3390/ijms20215465 |
_version_ | 1783471515120959488 |
---|---|
author | Meidani, Christianna Ntalli, Nikoletta G. Giannoutsou, Eleni Adamakis, Ioannis-Dimosthenis S. |
author_facet | Meidani, Christianna Ntalli, Nikoletta G. Giannoutsou, Eleni Adamakis, Ioannis-Dimosthenis S. |
author_sort | Meidani, Christianna |
collection | PubMed |
description | Meloidogyne incognita is a root knot nematode (RKN) species which is among the most notoriously unmanageable crop pests with a wide host range. It inhabits plants and induces unique feeding site structures within host roots, known as giant cells (GCs). The cell walls of the GCs undergo the process of both thickening and loosening to allow expansion and finally support nutrient uptake by the nematode. In this study, a comparative in situ analysis of cell wall polysaccharides in the GCs of wild-type Col-0 and the microtubule-defective fra2 katanin mutant, both infected with M. incognita has been carried out. The fra2 mutant had an increased infection rate. Moreover, fra2 roots exhibited a differential pectin and hemicellulose distribution when compared to Col-0 probably mirroring the fra2 root developmental defects. Features of fra2 GC walls include the presence of high-esterified pectic homogalacturonan and pectic arabinan, possibly to compensate for the reduced levels of callose, which was omnipresent in GCs of Col-0. Katanin severing of microtubules seems important in plant defense against M. incognita, with the nematode, however, to be nonchalant about this “katanin deficiency” and eventually induce the necessary GC cell wall modifications to establish a feeding site. |
format | Online Article Text |
id | pubmed-6862268 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68622682019-12-05 Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2 Arabidopsis thaliana Katanin Mutant Meidani, Christianna Ntalli, Nikoletta G. Giannoutsou, Eleni Adamakis, Ioannis-Dimosthenis S. Int J Mol Sci Article Meloidogyne incognita is a root knot nematode (RKN) species which is among the most notoriously unmanageable crop pests with a wide host range. It inhabits plants and induces unique feeding site structures within host roots, known as giant cells (GCs). The cell walls of the GCs undergo the process of both thickening and loosening to allow expansion and finally support nutrient uptake by the nematode. In this study, a comparative in situ analysis of cell wall polysaccharides in the GCs of wild-type Col-0 and the microtubule-defective fra2 katanin mutant, both infected with M. incognita has been carried out. The fra2 mutant had an increased infection rate. Moreover, fra2 roots exhibited a differential pectin and hemicellulose distribution when compared to Col-0 probably mirroring the fra2 root developmental defects. Features of fra2 GC walls include the presence of high-esterified pectic homogalacturonan and pectic arabinan, possibly to compensate for the reduced levels of callose, which was omnipresent in GCs of Col-0. Katanin severing of microtubules seems important in plant defense against M. incognita, with the nematode, however, to be nonchalant about this “katanin deficiency” and eventually induce the necessary GC cell wall modifications to establish a feeding site. MDPI 2019-11-02 /pmc/articles/PMC6862268/ /pubmed/31684028 http://dx.doi.org/10.3390/ijms20215465 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Meidani, Christianna Ntalli, Nikoletta G. Giannoutsou, Eleni Adamakis, Ioannis-Dimosthenis S. Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2 Arabidopsis thaliana Katanin Mutant |
title | Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2
Arabidopsis thaliana Katanin Mutant |
title_full | Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2
Arabidopsis thaliana Katanin Mutant |
title_fullStr | Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2
Arabidopsis thaliana Katanin Mutant |
title_full_unstemmed | Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2
Arabidopsis thaliana Katanin Mutant |
title_short | Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2
Arabidopsis thaliana Katanin Mutant |
title_sort | cell wall modifications in giant cells induced by the plant parasitic nematode meloidogyne incognita in wild-type (col-0) and the fra2
arabidopsis thaliana katanin mutant |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862268/ https://www.ncbi.nlm.nih.gov/pubmed/31684028 http://dx.doi.org/10.3390/ijms20215465 |
work_keys_str_mv | AT meidanichristianna cellwallmodificationsingiantcellsinducedbytheplantparasiticnematodemeloidogyneincognitainwildtypecol0andthefra2arabidopsisthalianakataninmutant AT ntallinikolettag cellwallmodificationsingiantcellsinducedbytheplantparasiticnematodemeloidogyneincognitainwildtypecol0andthefra2arabidopsisthalianakataninmutant AT giannoutsoueleni cellwallmodificationsingiantcellsinducedbytheplantparasiticnematodemeloidogyneincognitainwildtypecol0andthefra2arabidopsisthalianakataninmutant AT adamakisioannisdimostheniss cellwallmodificationsingiantcellsinducedbytheplantparasiticnematodemeloidogyneincognitainwildtypecol0andthefra2arabidopsisthalianakataninmutant |