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Reduced Cholinergic Activity in the Hippocampus of Hippocampal Cholinergic Neurostimulating Peptide Precursor Protein Knockout Mice

The cholinergic efferent network from the medial septal nucleus to the hippocampus has an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to efficiently encode novel information. Hippocampal cholinergic neurostimulating...

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Autores principales: Madokoro, Yuta, Yoshino, Yuta, Kato, Daisuke, Sato, Toyohiro, Mizuno, Masayuki, Kanamori, Tetsuko, Shimazawa, Masamitsu, Hida, Hideki, Hara, Hideaki, Yoshida, Mari, Borlongan, Cesario V., Ojika, Kosei, Matsukawa, Noriyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862429/
https://www.ncbi.nlm.nih.gov/pubmed/31661900
http://dx.doi.org/10.3390/ijms20215367
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author Madokoro, Yuta
Yoshino, Yuta
Kato, Daisuke
Sato, Toyohiro
Mizuno, Masayuki
Kanamori, Tetsuko
Shimazawa, Masamitsu
Hida, Hideki
Hara, Hideaki
Yoshida, Mari
Borlongan, Cesario V.
Ojika, Kosei
Matsukawa, Noriyuki
author_facet Madokoro, Yuta
Yoshino, Yuta
Kato, Daisuke
Sato, Toyohiro
Mizuno, Masayuki
Kanamori, Tetsuko
Shimazawa, Masamitsu
Hida, Hideki
Hara, Hideaki
Yoshida, Mari
Borlongan, Cesario V.
Ojika, Kosei
Matsukawa, Noriyuki
author_sort Madokoro, Yuta
collection PubMed
description The cholinergic efferent network from the medial septal nucleus to the hippocampus has an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to efficiently encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP) induces acetylcholine synthesis in medial septal nuclei. HCNP is processed from the N-terminal region of a 186 amino acid, 21 kD HCNP precursor protein called HCNP-pp (also known as Raf kinase inhibitory protein (RKIP) and phosphatidylethanolamine-binding protein 1 (PEBP1)). In this study, we generated HCNP-pp knockout (KO) mice and assessed their cholinergic septo-hippocampal projection, local field potentials in CA1, and behavioral phenotypes. No significant behavioral phenotype was observed in HCNP-pp KO mice. However, theta power in the CA1 of HCNP-pp KO mice was significantly reduced because of fewer cholineacetyltransferase-positive axons in the CA1 stratum oriens. These observations indicated disruption of cholinergic activity in the septo-hippocampal network. Our study demonstrates that HCNP may be a cholinergic regulator in the septo-hippocampal network.
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spelling pubmed-68624292019-12-05 Reduced Cholinergic Activity in the Hippocampus of Hippocampal Cholinergic Neurostimulating Peptide Precursor Protein Knockout Mice Madokoro, Yuta Yoshino, Yuta Kato, Daisuke Sato, Toyohiro Mizuno, Masayuki Kanamori, Tetsuko Shimazawa, Masamitsu Hida, Hideki Hara, Hideaki Yoshida, Mari Borlongan, Cesario V. Ojika, Kosei Matsukawa, Noriyuki Int J Mol Sci Article The cholinergic efferent network from the medial septal nucleus to the hippocampus has an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to efficiently encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP) induces acetylcholine synthesis in medial septal nuclei. HCNP is processed from the N-terminal region of a 186 amino acid, 21 kD HCNP precursor protein called HCNP-pp (also known as Raf kinase inhibitory protein (RKIP) and phosphatidylethanolamine-binding protein 1 (PEBP1)). In this study, we generated HCNP-pp knockout (KO) mice and assessed their cholinergic septo-hippocampal projection, local field potentials in CA1, and behavioral phenotypes. No significant behavioral phenotype was observed in HCNP-pp KO mice. However, theta power in the CA1 of HCNP-pp KO mice was significantly reduced because of fewer cholineacetyltransferase-positive axons in the CA1 stratum oriens. These observations indicated disruption of cholinergic activity in the septo-hippocampal network. Our study demonstrates that HCNP may be a cholinergic regulator in the septo-hippocampal network. MDPI 2019-10-28 /pmc/articles/PMC6862429/ /pubmed/31661900 http://dx.doi.org/10.3390/ijms20215367 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Madokoro, Yuta
Yoshino, Yuta
Kato, Daisuke
Sato, Toyohiro
Mizuno, Masayuki
Kanamori, Tetsuko
Shimazawa, Masamitsu
Hida, Hideki
Hara, Hideaki
Yoshida, Mari
Borlongan, Cesario V.
Ojika, Kosei
Matsukawa, Noriyuki
Reduced Cholinergic Activity in the Hippocampus of Hippocampal Cholinergic Neurostimulating Peptide Precursor Protein Knockout Mice
title Reduced Cholinergic Activity in the Hippocampus of Hippocampal Cholinergic Neurostimulating Peptide Precursor Protein Knockout Mice
title_full Reduced Cholinergic Activity in the Hippocampus of Hippocampal Cholinergic Neurostimulating Peptide Precursor Protein Knockout Mice
title_fullStr Reduced Cholinergic Activity in the Hippocampus of Hippocampal Cholinergic Neurostimulating Peptide Precursor Protein Knockout Mice
title_full_unstemmed Reduced Cholinergic Activity in the Hippocampus of Hippocampal Cholinergic Neurostimulating Peptide Precursor Protein Knockout Mice
title_short Reduced Cholinergic Activity in the Hippocampus of Hippocampal Cholinergic Neurostimulating Peptide Precursor Protein Knockout Mice
title_sort reduced cholinergic activity in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862429/
https://www.ncbi.nlm.nih.gov/pubmed/31661900
http://dx.doi.org/10.3390/ijms20215367
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