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A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery

In contrast to many protein translocases that use ATP or GTP hydrolysis as the driving force to transport proteins across biological membranes, the peroxisomal matrix protein import machinery relies on a regulated self-assembly mechanism for this purpose and uses ATP hydrolysis only to reset its com...

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Detalles Bibliográficos
Autores principales: Pedrosa, Ana G., Francisco, Tânia, Ferreira, Maria J., Rodrigues, Tony A., Barros-Barbosa, Aurora, Azevedo, Jorge E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862443/
https://www.ncbi.nlm.nih.gov/pubmed/31652724
http://dx.doi.org/10.3390/ijms20215246
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author Pedrosa, Ana G.
Francisco, Tânia
Ferreira, Maria J.
Rodrigues, Tony A.
Barros-Barbosa, Aurora
Azevedo, Jorge E.
author_facet Pedrosa, Ana G.
Francisco, Tânia
Ferreira, Maria J.
Rodrigues, Tony A.
Barros-Barbosa, Aurora
Azevedo, Jorge E.
author_sort Pedrosa, Ana G.
collection PubMed
description In contrast to many protein translocases that use ATP or GTP hydrolysis as the driving force to transport proteins across biological membranes, the peroxisomal matrix protein import machinery relies on a regulated self-assembly mechanism for this purpose and uses ATP hydrolysis only to reset its components. The ATP-dependent protein complex in charge of resetting this machinery—the Receptor Export Module (REM)—comprises two members of the “ATPases Associated with diverse cellular Activities” (AAA+) family, PEX1 and PEX6, and a membrane protein that anchors the ATPases to the organelle membrane. In recent years, a large amount of data on the structure/function of the REM complex has become available. Here, we discuss the main findings and their mechanistic implications.
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spelling pubmed-68624432019-12-05 A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery Pedrosa, Ana G. Francisco, Tânia Ferreira, Maria J. Rodrigues, Tony A. Barros-Barbosa, Aurora Azevedo, Jorge E. Int J Mol Sci Review In contrast to many protein translocases that use ATP or GTP hydrolysis as the driving force to transport proteins across biological membranes, the peroxisomal matrix protein import machinery relies on a regulated self-assembly mechanism for this purpose and uses ATP hydrolysis only to reset its components. The ATP-dependent protein complex in charge of resetting this machinery—the Receptor Export Module (REM)—comprises two members of the “ATPases Associated with diverse cellular Activities” (AAA+) family, PEX1 and PEX6, and a membrane protein that anchors the ATPases to the organelle membrane. In recent years, a large amount of data on the structure/function of the REM complex has become available. Here, we discuss the main findings and their mechanistic implications. MDPI 2019-10-23 /pmc/articles/PMC6862443/ /pubmed/31652724 http://dx.doi.org/10.3390/ijms20215246 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Pedrosa, Ana G.
Francisco, Tânia
Ferreira, Maria J.
Rodrigues, Tony A.
Barros-Barbosa, Aurora
Azevedo, Jorge E.
A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery
title A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery
title_full A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery
title_fullStr A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery
title_full_unstemmed A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery
title_short A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery
title_sort mechanistic perspective on pex1 and pex6, two aaa+ proteins of the peroxisomal protein import machinery
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862443/
https://www.ncbi.nlm.nih.gov/pubmed/31652724
http://dx.doi.org/10.3390/ijms20215246
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