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A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery
In contrast to many protein translocases that use ATP or GTP hydrolysis as the driving force to transport proteins across biological membranes, the peroxisomal matrix protein import machinery relies on a regulated self-assembly mechanism for this purpose and uses ATP hydrolysis only to reset its com...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862443/ https://www.ncbi.nlm.nih.gov/pubmed/31652724 http://dx.doi.org/10.3390/ijms20215246 |
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author | Pedrosa, Ana G. Francisco, Tânia Ferreira, Maria J. Rodrigues, Tony A. Barros-Barbosa, Aurora Azevedo, Jorge E. |
author_facet | Pedrosa, Ana G. Francisco, Tânia Ferreira, Maria J. Rodrigues, Tony A. Barros-Barbosa, Aurora Azevedo, Jorge E. |
author_sort | Pedrosa, Ana G. |
collection | PubMed |
description | In contrast to many protein translocases that use ATP or GTP hydrolysis as the driving force to transport proteins across biological membranes, the peroxisomal matrix protein import machinery relies on a regulated self-assembly mechanism for this purpose and uses ATP hydrolysis only to reset its components. The ATP-dependent protein complex in charge of resetting this machinery—the Receptor Export Module (REM)—comprises two members of the “ATPases Associated with diverse cellular Activities” (AAA+) family, PEX1 and PEX6, and a membrane protein that anchors the ATPases to the organelle membrane. In recent years, a large amount of data on the structure/function of the REM complex has become available. Here, we discuss the main findings and their mechanistic implications. |
format | Online Article Text |
id | pubmed-6862443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68624432019-12-05 A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery Pedrosa, Ana G. Francisco, Tânia Ferreira, Maria J. Rodrigues, Tony A. Barros-Barbosa, Aurora Azevedo, Jorge E. Int J Mol Sci Review In contrast to many protein translocases that use ATP or GTP hydrolysis as the driving force to transport proteins across biological membranes, the peroxisomal matrix protein import machinery relies on a regulated self-assembly mechanism for this purpose and uses ATP hydrolysis only to reset its components. The ATP-dependent protein complex in charge of resetting this machinery—the Receptor Export Module (REM)—comprises two members of the “ATPases Associated with diverse cellular Activities” (AAA+) family, PEX1 and PEX6, and a membrane protein that anchors the ATPases to the organelle membrane. In recent years, a large amount of data on the structure/function of the REM complex has become available. Here, we discuss the main findings and their mechanistic implications. MDPI 2019-10-23 /pmc/articles/PMC6862443/ /pubmed/31652724 http://dx.doi.org/10.3390/ijms20215246 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Pedrosa, Ana G. Francisco, Tânia Ferreira, Maria J. Rodrigues, Tony A. Barros-Barbosa, Aurora Azevedo, Jorge E. A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery |
title | A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery |
title_full | A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery |
title_fullStr | A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery |
title_full_unstemmed | A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery |
title_short | A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery |
title_sort | mechanistic perspective on pex1 and pex6, two aaa+ proteins of the peroxisomal protein import machinery |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862443/ https://www.ncbi.nlm.nih.gov/pubmed/31652724 http://dx.doi.org/10.3390/ijms20215246 |
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