Cross-species validation of human specific STR system, SureID(®)21G and SureID(®)23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes)

OBJECTIVES: The human specific commercially available STRs system are often not tested in non human primates for their cross applicability. The aim of this study is to test Cross-species validation of two commercially available human specific STR kits i.e. SureID(®) 21G and SureID(®) 23comp (Health...

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Autores principales: Singh, Abhishek, Thakur, Mukesh, Sahajpal, Vivek, Singh, Sujeet K., Chandra, Kailash, Sharma, Arun, Devi, Nisha, Bernot, Ausma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862757/
https://www.ncbi.nlm.nih.gov/pubmed/31744541
http://dx.doi.org/10.1186/s13104-019-4801-3
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author Singh, Abhishek
Thakur, Mukesh
Sahajpal, Vivek
Singh, Sujeet K.
Chandra, Kailash
Sharma, Arun
Devi, Nisha
Bernot, Ausma
author_facet Singh, Abhishek
Thakur, Mukesh
Sahajpal, Vivek
Singh, Sujeet K.
Chandra, Kailash
Sharma, Arun
Devi, Nisha
Bernot, Ausma
author_sort Singh, Abhishek
collection PubMed
description OBJECTIVES: The human specific commercially available STRs system are often not tested in non human primates for their cross applicability. The aim of this study is to test Cross-species validation of two commercially available human specific STR kits i.e. SureID(®) 21G and SureID(®) 23comp (Health Gene Technologies) for their positive application in Chimpanzee (Pan troglodytes). RESULT: In SureID(®) 21G, 19 loci amplified and while 20 loci amplified in SureID(®) 23comp. All the amplified loci in both STR kits were found polymorphic and the locus Amelogenin showed differential banding patterns between male and female revealing their known gender. The present study validates the applicability of these human specific STR kits in Chimpanzee that can be used in forensics analysis, paternity testing and population genetic studies.
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spelling pubmed-68627572019-12-11 Cross-species validation of human specific STR system, SureID(®)21G and SureID(®)23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes) Singh, Abhishek Thakur, Mukesh Sahajpal, Vivek Singh, Sujeet K. Chandra, Kailash Sharma, Arun Devi, Nisha Bernot, Ausma BMC Res Notes Research Note OBJECTIVES: The human specific commercially available STRs system are often not tested in non human primates for their cross applicability. The aim of this study is to test Cross-species validation of two commercially available human specific STR kits i.e. SureID(®) 21G and SureID(®) 23comp (Health Gene Technologies) for their positive application in Chimpanzee (Pan troglodytes). RESULT: In SureID(®) 21G, 19 loci amplified and while 20 loci amplified in SureID(®) 23comp. All the amplified loci in both STR kits were found polymorphic and the locus Amelogenin showed differential banding patterns between male and female revealing their known gender. The present study validates the applicability of these human specific STR kits in Chimpanzee that can be used in forensics analysis, paternity testing and population genetic studies. BioMed Central 2019-11-19 /pmc/articles/PMC6862757/ /pubmed/31744541 http://dx.doi.org/10.1186/s13104-019-4801-3 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Singh, Abhishek
Thakur, Mukesh
Sahajpal, Vivek
Singh, Sujeet K.
Chandra, Kailash
Sharma, Arun
Devi, Nisha
Bernot, Ausma
Cross-species validation of human specific STR system, SureID(®)21G and SureID(®)23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes)
title Cross-species validation of human specific STR system, SureID(®)21G and SureID(®)23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes)
title_full Cross-species validation of human specific STR system, SureID(®)21G and SureID(®)23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes)
title_fullStr Cross-species validation of human specific STR system, SureID(®)21G and SureID(®)23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes)
title_full_unstemmed Cross-species validation of human specific STR system, SureID(®)21G and SureID(®)23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes)
title_short Cross-species validation of human specific STR system, SureID(®)21G and SureID(®)23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes)
title_sort cross-species validation of human specific str system, sureid(®)21g and sureid(®)23comp (health gene technologies) in chimpanzee (pan troglodytes)
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862757/
https://www.ncbi.nlm.nih.gov/pubmed/31744541
http://dx.doi.org/10.1186/s13104-019-4801-3
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