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Naive B Cell Output in HIV-Infected and HIV-Uninfected Children

In this study, we aimed to quantify KREC (kappa-deleting recombination excision circle) levels and naive B cell output in healthy HIV-uninfected children, compared with HIV-infected South African children, before and after starting ART (antiretroviral therapy). Samples were acquired from a Child Wel...

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Autores principales: Payne, Helen, Chain, Gabriel, Adams, Stuart, Hunter, Patricia, Luckhurst, Natasha, Gilmour, Kimberly, Lewis, Joanna, Babiker, Abdel, Cotton, Mark, Violari, Avy, Gibb, Diana, Callard, Robin, Klein, Nigel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6863188/
https://www.ncbi.nlm.nih.gov/pubmed/30298747
http://dx.doi.org/10.1089/aid.2018.0170
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author Payne, Helen
Chain, Gabriel
Adams, Stuart
Hunter, Patricia
Luckhurst, Natasha
Gilmour, Kimberly
Lewis, Joanna
Babiker, Abdel
Cotton, Mark
Violari, Avy
Gibb, Diana
Callard, Robin
Klein, Nigel
author_facet Payne, Helen
Chain, Gabriel
Adams, Stuart
Hunter, Patricia
Luckhurst, Natasha
Gilmour, Kimberly
Lewis, Joanna
Babiker, Abdel
Cotton, Mark
Violari, Avy
Gibb, Diana
Callard, Robin
Klein, Nigel
author_sort Payne, Helen
collection PubMed
description In this study, we aimed to quantify KREC (kappa-deleting recombination excision circle) levels and naive B cell output in healthy HIV-uninfected children, compared with HIV-infected South African children, before and after starting ART (antiretroviral therapy). Samples were acquired from a Child Wellness Clinic (n = 288 HIV-uninfected South African children, 2 weeks–12 years) and the Children with HIV Early Antiretroviral Therapy (CHER) trial (n = 153 HIV-infected South African children, 7 weeks–8 years). Naive B cell output was estimated using a mathematical model combining KREC levels to reflect B cell emigration into the circulation, flow cytometry measures of naive unswitched B cells to quantify total body naive B cells, and their rates of proliferation using the intracellular marker Ki67. Naive B cell output increases from birth to 1 year, followed by a decline and plateau into late childhood. HIV-infected children on or off ART had higher naive B cell outputs than their uninfected counterparts (p = .01 and p = .04). This is the first study to present reference ranges for measurements of KRECs and naive B cell output in healthy and HIV-infected children. Comparison between HIV-uninfected healthy children and HIV-infected children suggests that HIV may increase naive B cell output. Further work is required to fully understand the mechanisms involved and clinical value of measuring naive B cell output in children.
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spelling pubmed-68631882019-11-20 Naive B Cell Output in HIV-Infected and HIV-Uninfected Children Payne, Helen Chain, Gabriel Adams, Stuart Hunter, Patricia Luckhurst, Natasha Gilmour, Kimberly Lewis, Joanna Babiker, Abdel Cotton, Mark Violari, Avy Gibb, Diana Callard, Robin Klein, Nigel AIDS Res Hum Retroviruses Immunology In this study, we aimed to quantify KREC (kappa-deleting recombination excision circle) levels and naive B cell output in healthy HIV-uninfected children, compared with HIV-infected South African children, before and after starting ART (antiretroviral therapy). Samples were acquired from a Child Wellness Clinic (n = 288 HIV-uninfected South African children, 2 weeks–12 years) and the Children with HIV Early Antiretroviral Therapy (CHER) trial (n = 153 HIV-infected South African children, 7 weeks–8 years). Naive B cell output was estimated using a mathematical model combining KREC levels to reflect B cell emigration into the circulation, flow cytometry measures of naive unswitched B cells to quantify total body naive B cells, and their rates of proliferation using the intracellular marker Ki67. Naive B cell output increases from birth to 1 year, followed by a decline and plateau into late childhood. HIV-infected children on or off ART had higher naive B cell outputs than their uninfected counterparts (p = .01 and p = .04). This is the first study to present reference ranges for measurements of KRECs and naive B cell output in healthy and HIV-infected children. Comparison between HIV-uninfected healthy children and HIV-infected children suggests that HIV may increase naive B cell output. Further work is required to fully understand the mechanisms involved and clinical value of measuring naive B cell output in children. Mary Ann Liebert, Inc., publishers 2019-01-01 2019-02-26 /pmc/articles/PMC6863188/ /pubmed/30298747 http://dx.doi.org/10.1089/aid.2018.0170 Text en © Helen Payne et al. 2019; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Immunology
Payne, Helen
Chain, Gabriel
Adams, Stuart
Hunter, Patricia
Luckhurst, Natasha
Gilmour, Kimberly
Lewis, Joanna
Babiker, Abdel
Cotton, Mark
Violari, Avy
Gibb, Diana
Callard, Robin
Klein, Nigel
Naive B Cell Output in HIV-Infected and HIV-Uninfected Children
title Naive B Cell Output in HIV-Infected and HIV-Uninfected Children
title_full Naive B Cell Output in HIV-Infected and HIV-Uninfected Children
title_fullStr Naive B Cell Output in HIV-Infected and HIV-Uninfected Children
title_full_unstemmed Naive B Cell Output in HIV-Infected and HIV-Uninfected Children
title_short Naive B Cell Output in HIV-Infected and HIV-Uninfected Children
title_sort naive b cell output in hiv-infected and hiv-uninfected children
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6863188/
https://www.ncbi.nlm.nih.gov/pubmed/30298747
http://dx.doi.org/10.1089/aid.2018.0170
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