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Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo

Coronaviruses are single-stranded positive-sense RNA viruses associated with important avian diseases. Their relatively high rates of mutation and recombination frequencies allow them to adapt to new hosts and ecological niches. Although Brazil has 18% of global avian species diversity, studies rega...

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Autores principales: Barbosa, Carla M., Durigon, Edison L., Thomazelli, Luciano M., Ometto, Tatiana, Marcatti, Roberta, Nardi, Marcello Shiavo, de Aguiar, Daniel M., Pinho, João Batista, Petry, Maria Virginia, Neto, Isaac Simão, Serafini, Patrícia, Rodrigues, Roberta Costa, de Azevedo Junior, Severino Mendes, Góes, Luiz Gustavo B., de Araujo, Jansen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6863219/
https://www.ncbi.nlm.nih.gov/pubmed/30877663
http://dx.doi.org/10.1007/s42770-019-00065-7
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author Barbosa, Carla M.
Durigon, Edison L.
Thomazelli, Luciano M.
Ometto, Tatiana
Marcatti, Roberta
Nardi, Marcello Shiavo
de Aguiar, Daniel M.
Pinho, João Batista
Petry, Maria Virginia
Neto, Isaac Simão
Serafini, Patrícia
Rodrigues, Roberta Costa
de Azevedo Junior, Severino Mendes
Góes, Luiz Gustavo B.
de Araujo, Jansen
author_facet Barbosa, Carla M.
Durigon, Edison L.
Thomazelli, Luciano M.
Ometto, Tatiana
Marcatti, Roberta
Nardi, Marcello Shiavo
de Aguiar, Daniel M.
Pinho, João Batista
Petry, Maria Virginia
Neto, Isaac Simão
Serafini, Patrícia
Rodrigues, Roberta Costa
de Azevedo Junior, Severino Mendes
Góes, Luiz Gustavo B.
de Araujo, Jansen
author_sort Barbosa, Carla M.
collection PubMed
description Coronaviruses are single-stranded positive-sense RNA viruses associated with important avian diseases. Their relatively high rates of mutation and recombination frequencies allow them to adapt to new hosts and ecological niches. Although Brazil has 18% of global avian species diversity, studies regarding the presence of avian viral diseases in wild birds in South America are scarce. In this study, we performed a retrospective analysis of the presence of CoVs in 746 wild birds. Oropharyngeal and cloacal swabs were obtained and placed together in vials containing VTM transport medium collected in different regions of Brazil between 2006 and 2013. Screening for viral nucleic acid was performed using conventional RT-PCR and pancoronavirus nested PCR. Positive samples were characterized by partial sequencing of the RNA-dependent RNA polymerase (RdRp) gene, and ensuing phylogenetic analysis was performed to investigate the association between virus epidemiology and bird migration routes. Coronavirus RNA were detected and sequenced from six samples, in which three were related to gammacoronaviruses group and the other three to deltacoronavirus group. Our study documents the presence of CoVs related to avian gamma- and deltacoronaviruses circulating in both urban- and poultry-farm regions of Brazil, implicating wild birds as potential carriers of CoVs which may represent a risk to poultry farms and public health in Brazil. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s42770-019-00065-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-68632192020-03-15 Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo Barbosa, Carla M. Durigon, Edison L. Thomazelli, Luciano M. Ometto, Tatiana Marcatti, Roberta Nardi, Marcello Shiavo de Aguiar, Daniel M. Pinho, João Batista Petry, Maria Virginia Neto, Isaac Simão Serafini, Patrícia Rodrigues, Roberta Costa de Azevedo Junior, Severino Mendes Góes, Luiz Gustavo B. de Araujo, Jansen Braz J Microbiol Veterinary Microbiology - Research Paper Coronaviruses are single-stranded positive-sense RNA viruses associated with important avian diseases. Their relatively high rates of mutation and recombination frequencies allow them to adapt to new hosts and ecological niches. Although Brazil has 18% of global avian species diversity, studies regarding the presence of avian viral diseases in wild birds in South America are scarce. In this study, we performed a retrospective analysis of the presence of CoVs in 746 wild birds. Oropharyngeal and cloacal swabs were obtained and placed together in vials containing VTM transport medium collected in different regions of Brazil between 2006 and 2013. Screening for viral nucleic acid was performed using conventional RT-PCR and pancoronavirus nested PCR. Positive samples were characterized by partial sequencing of the RNA-dependent RNA polymerase (RdRp) gene, and ensuing phylogenetic analysis was performed to investigate the association between virus epidemiology and bird migration routes. Coronavirus RNA were detected and sequenced from six samples, in which three were related to gammacoronaviruses group and the other three to deltacoronavirus group. Our study documents the presence of CoVs related to avian gamma- and deltacoronaviruses circulating in both urban- and poultry-farm regions of Brazil, implicating wild birds as potential carriers of CoVs which may represent a risk to poultry farms and public health in Brazil. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s42770-019-00065-7) contains supplementary material, which is available to authorized users. Springer International Publishing 2019-03-15 /pmc/articles/PMC6863219/ /pubmed/30877663 http://dx.doi.org/10.1007/s42770-019-00065-7 Text en © Sociedade Brasileira de Microbiologia 2019
spellingShingle Veterinary Microbiology - Research Paper
Barbosa, Carla M.
Durigon, Edison L.
Thomazelli, Luciano M.
Ometto, Tatiana
Marcatti, Roberta
Nardi, Marcello Shiavo
de Aguiar, Daniel M.
Pinho, João Batista
Petry, Maria Virginia
Neto, Isaac Simão
Serafini, Patrícia
Rodrigues, Roberta Costa
de Azevedo Junior, Severino Mendes
Góes, Luiz Gustavo B.
de Araujo, Jansen
Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo
title Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo
title_full Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo
title_fullStr Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo
title_full_unstemmed Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo
title_short Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo
title_sort divergent coronaviruses detected in wild birds in brazil, including a central park in são paulo
topic Veterinary Microbiology - Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6863219/
https://www.ncbi.nlm.nih.gov/pubmed/30877663
http://dx.doi.org/10.1007/s42770-019-00065-7
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