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Length regulation of multiple flagella that self-assemble from a shared pool of components

The single-celled green algae Chlamydomonas reinhardtii with its two flagella—microtubule-based structures of equal and constant lengths—is the canonical model organism for studying size control of organelles. Experiments have identified motor-driven transport of tubulin to the flagella tips as a ke...

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Autores principales: Fai, Thomas G, Mohapatra, Lishibanya, Kar, Prathitha, Kondev, Jane, Amir, Ariel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6863624/
https://www.ncbi.nlm.nih.gov/pubmed/31596235
http://dx.doi.org/10.7554/eLife.42599
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author Fai, Thomas G
Mohapatra, Lishibanya
Kar, Prathitha
Kondev, Jane
Amir, Ariel
author_facet Fai, Thomas G
Mohapatra, Lishibanya
Kar, Prathitha
Kondev, Jane
Amir, Ariel
author_sort Fai, Thomas G
collection PubMed
description The single-celled green algae Chlamydomonas reinhardtii with its two flagella—microtubule-based structures of equal and constant lengths—is the canonical model organism for studying size control of organelles. Experiments have identified motor-driven transport of tubulin to the flagella tips as a key component of their length control. Here we consider a class of models whose key assumption is that proteins responsible for the intraflagellar transport (IFT) of tubulin are present in limiting amounts. We show that the limiting-pool assumption is insufficient to describe the results of severing experiments, in which a flagellum is regenerated after it has been severed. Next, we consider an extension of the limiting-pool model that incorporates proteins that depolymerize microtubules. We show that this ‘active disassembly’ model of flagellar length control explains in quantitative detail the results of severing experiments and use it to make predictions that can be tested in experiments.
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spelling pubmed-68636242019-11-20 Length regulation of multiple flagella that self-assemble from a shared pool of components Fai, Thomas G Mohapatra, Lishibanya Kar, Prathitha Kondev, Jane Amir, Ariel eLife Physics of Living Systems The single-celled green algae Chlamydomonas reinhardtii with its two flagella—microtubule-based structures of equal and constant lengths—is the canonical model organism for studying size control of organelles. Experiments have identified motor-driven transport of tubulin to the flagella tips as a key component of their length control. Here we consider a class of models whose key assumption is that proteins responsible for the intraflagellar transport (IFT) of tubulin are present in limiting amounts. We show that the limiting-pool assumption is insufficient to describe the results of severing experiments, in which a flagellum is regenerated after it has been severed. Next, we consider an extension of the limiting-pool model that incorporates proteins that depolymerize microtubules. We show that this ‘active disassembly’ model of flagellar length control explains in quantitative detail the results of severing experiments and use it to make predictions that can be tested in experiments. eLife Sciences Publications, Ltd 2019-10-09 /pmc/articles/PMC6863624/ /pubmed/31596235 http://dx.doi.org/10.7554/eLife.42599 Text en © 2019, Fai et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Physics of Living Systems
Fai, Thomas G
Mohapatra, Lishibanya
Kar, Prathitha
Kondev, Jane
Amir, Ariel
Length regulation of multiple flagella that self-assemble from a shared pool of components
title Length regulation of multiple flagella that self-assemble from a shared pool of components
title_full Length regulation of multiple flagella that self-assemble from a shared pool of components
title_fullStr Length regulation of multiple flagella that self-assemble from a shared pool of components
title_full_unstemmed Length regulation of multiple flagella that self-assemble from a shared pool of components
title_short Length regulation of multiple flagella that self-assemble from a shared pool of components
title_sort length regulation of multiple flagella that self-assemble from a shared pool of components
topic Physics of Living Systems
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6863624/
https://www.ncbi.nlm.nih.gov/pubmed/31596235
http://dx.doi.org/10.7554/eLife.42599
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