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A Study on actinobacterial diversity of Hampoeil cave and screening of their biological activities

Caves are oligotrophic, dark and less-explored environments and are considered as sources of promising microbial strains in biotechnology. Hampoeil Cave is located in massive dolomite with thin bedded limestone in northwestern of Iran. In an isolation and screening program, various samples from soil...

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Detalles Bibliográficos
Autores principales: Hamedi, Javad, Kafshnouchi, Maghsoud, Ranjbaran, Mohsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864206/
https://www.ncbi.nlm.nih.gov/pubmed/31762631
http://dx.doi.org/10.1016/j.sjbs.2018.10.010
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author Hamedi, Javad
Kafshnouchi, Maghsoud
Ranjbaran, Mohsen
author_facet Hamedi, Javad
Kafshnouchi, Maghsoud
Ranjbaran, Mohsen
author_sort Hamedi, Javad
collection PubMed
description Caves are oligotrophic, dark and less-explored environments and are considered as sources of promising microbial strains in biotechnology. Hampoeil Cave is located in massive dolomite with thin bedded limestone in northwestern of Iran. In an isolation and screening program, various samples from soil, water, floor, wall and ceiling of Hampoeil cave and its invertebrates were collected. Four various treatments and 10 different isolation media were used for the isolation of the actinobacteria. Screening of the isolates for antimicrobial activity against 10 bacteria and fungi, 5 hydrolytic enzymes production and resistance to 5 heavy metals have been performed. Among 33 various samples, 76 actinobacteria from various genera, including Streptomyces, Micromonospora, Micrococcus, Kocuria and Corynebacterium were isolated. Eighty percent of the strains had one of the studied hydrolytic enzyme activity. At least one type of antimicrobial activity was seen in 25.3% of the isolates. Resistance to one metal or more was seen in 26.32% of the isolates. The ratio of rare-actinobacteria in the oligotrophic samples to enriched samples is 20% more than Streptomyces. Percentage of strains with the highest activity in esterase, amylase, DNase, protease or lipase activity that were isolated from organic-rich environmental samples were 100, 100, 100, 82 and 82%, respectively. Also, 26.32% of the actinobacterial isolates resisted to heavy metals. It was concluded that Hampoeil cave is a good source in finding cave-living actinobacteria potent in producing hydrolytic enzymes and bioremediation.
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spelling pubmed-68642062019-11-22 A Study on actinobacterial diversity of Hampoeil cave and screening of their biological activities Hamedi, Javad Kafshnouchi, Maghsoud Ranjbaran, Mohsen Saudi J Biol Sci Article Caves are oligotrophic, dark and less-explored environments and are considered as sources of promising microbial strains in biotechnology. Hampoeil Cave is located in massive dolomite with thin bedded limestone in northwestern of Iran. In an isolation and screening program, various samples from soil, water, floor, wall and ceiling of Hampoeil cave and its invertebrates were collected. Four various treatments and 10 different isolation media were used for the isolation of the actinobacteria. Screening of the isolates for antimicrobial activity against 10 bacteria and fungi, 5 hydrolytic enzymes production and resistance to 5 heavy metals have been performed. Among 33 various samples, 76 actinobacteria from various genera, including Streptomyces, Micromonospora, Micrococcus, Kocuria and Corynebacterium were isolated. Eighty percent of the strains had one of the studied hydrolytic enzyme activity. At least one type of antimicrobial activity was seen in 25.3% of the isolates. Resistance to one metal or more was seen in 26.32% of the isolates. The ratio of rare-actinobacteria in the oligotrophic samples to enriched samples is 20% more than Streptomyces. Percentage of strains with the highest activity in esterase, amylase, DNase, protease or lipase activity that were isolated from organic-rich environmental samples were 100, 100, 100, 82 and 82%, respectively. Also, 26.32% of the actinobacterial isolates resisted to heavy metals. It was concluded that Hampoeil cave is a good source in finding cave-living actinobacteria potent in producing hydrolytic enzymes and bioremediation. Elsevier 2019-11 2018-10-10 /pmc/articles/PMC6864206/ /pubmed/31762631 http://dx.doi.org/10.1016/j.sjbs.2018.10.010 Text en © 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Hamedi, Javad
Kafshnouchi, Maghsoud
Ranjbaran, Mohsen
A Study on actinobacterial diversity of Hampoeil cave and screening of their biological activities
title A Study on actinobacterial diversity of Hampoeil cave and screening of their biological activities
title_full A Study on actinobacterial diversity of Hampoeil cave and screening of their biological activities
title_fullStr A Study on actinobacterial diversity of Hampoeil cave and screening of their biological activities
title_full_unstemmed A Study on actinobacterial diversity of Hampoeil cave and screening of their biological activities
title_short A Study on actinobacterial diversity of Hampoeil cave and screening of their biological activities
title_sort study on actinobacterial diversity of hampoeil cave and screening of their biological activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864206/
https://www.ncbi.nlm.nih.gov/pubmed/31762631
http://dx.doi.org/10.1016/j.sjbs.2018.10.010
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