Cargando…

Tissue‐Engineered Neural Network Graft Relays Excitatory Signal in the Completely Transected Canine Spinal Cord

Tissue engineering produces constructs with defined functions for the targeted treatment of damaged tissue. A complete spinal cord injury (SCI) model is generated in canines to test whether in vitro constructed neural network (NN) tissues can relay the excitatory signal across the lesion gap to the...

Descripción completa

Detalles Bibliográficos
Autores principales: Lai, Bi‐Qin, Che, Ming‐Tian, Feng, Bo, Bai, Yu‐Rong, Li, Ge, Ma, Yuan‐Huan, Wang, Lai‐Jian, Huang, Meng‐Yao, Wang, Ya‐Qiong, Jiang, Bin, Ding, Ying, Zeng, Xiang, Zeng, Yuan‐Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864506/
https://www.ncbi.nlm.nih.gov/pubmed/31763143
http://dx.doi.org/10.1002/advs.201901240
_version_ 1783471898489782272
author Lai, Bi‐Qin
Che, Ming‐Tian
Feng, Bo
Bai, Yu‐Rong
Li, Ge
Ma, Yuan‐Huan
Wang, Lai‐Jian
Huang, Meng‐Yao
Wang, Ya‐Qiong
Jiang, Bin
Ding, Ying
Zeng, Xiang
Zeng, Yuan‐Shan
author_facet Lai, Bi‐Qin
Che, Ming‐Tian
Feng, Bo
Bai, Yu‐Rong
Li, Ge
Ma, Yuan‐Huan
Wang, Lai‐Jian
Huang, Meng‐Yao
Wang, Ya‐Qiong
Jiang, Bin
Ding, Ying
Zeng, Xiang
Zeng, Yuan‐Shan
author_sort Lai, Bi‐Qin
collection PubMed
description Tissue engineering produces constructs with defined functions for the targeted treatment of damaged tissue. A complete spinal cord injury (SCI) model is generated in canines to test whether in vitro constructed neural network (NN) tissues can relay the excitatory signal across the lesion gap to the caudal spinal cord. Established protocols are used to construct neural stem cell (NSC)‐derived NN tissue characterized by a predominantly neuronal population with robust trans‐synaptic activities and myelination. The NN tissue is implanted into the gap immediately following complete transection SCI of canines at the T10 spinal cord segment. The data show significant motor recovery of paralyzed pelvic limbs, as evaluated by Olby scoring and cortical motor evoked potential (CMEP) detection. The NN tissue survives in the lesion area with neuronal phenotype maintenance, improves descending and ascending nerve fiber regeneration, and synaptic integration with host neural circuits that allow it to serve as a neuronal relay to transmit excitatory electrical signal across the injured area to the caudal spinal cord. These results suggest that tissue‐engineered NN grafts can relay the excitatory signal in the completely transected canine spinal cord, providing a promising strategy for SCI treatment in large animals, including humans.
format Online
Article
Text
id pubmed-6864506
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-68645062019-11-22 Tissue‐Engineered Neural Network Graft Relays Excitatory Signal in the Completely Transected Canine Spinal Cord Lai, Bi‐Qin Che, Ming‐Tian Feng, Bo Bai, Yu‐Rong Li, Ge Ma, Yuan‐Huan Wang, Lai‐Jian Huang, Meng‐Yao Wang, Ya‐Qiong Jiang, Bin Ding, Ying Zeng, Xiang Zeng, Yuan‐Shan Adv Sci (Weinh) Full Papers Tissue engineering produces constructs with defined functions for the targeted treatment of damaged tissue. A complete spinal cord injury (SCI) model is generated in canines to test whether in vitro constructed neural network (NN) tissues can relay the excitatory signal across the lesion gap to the caudal spinal cord. Established protocols are used to construct neural stem cell (NSC)‐derived NN tissue characterized by a predominantly neuronal population with robust trans‐synaptic activities and myelination. The NN tissue is implanted into the gap immediately following complete transection SCI of canines at the T10 spinal cord segment. The data show significant motor recovery of paralyzed pelvic limbs, as evaluated by Olby scoring and cortical motor evoked potential (CMEP) detection. The NN tissue survives in the lesion area with neuronal phenotype maintenance, improves descending and ascending nerve fiber regeneration, and synaptic integration with host neural circuits that allow it to serve as a neuronal relay to transmit excitatory electrical signal across the injured area to the caudal spinal cord. These results suggest that tissue‐engineered NN grafts can relay the excitatory signal in the completely transected canine spinal cord, providing a promising strategy for SCI treatment in large animals, including humans. John Wiley and Sons Inc. 2019-09-19 /pmc/articles/PMC6864506/ /pubmed/31763143 http://dx.doi.org/10.1002/advs.201901240 Text en © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Lai, Bi‐Qin
Che, Ming‐Tian
Feng, Bo
Bai, Yu‐Rong
Li, Ge
Ma, Yuan‐Huan
Wang, Lai‐Jian
Huang, Meng‐Yao
Wang, Ya‐Qiong
Jiang, Bin
Ding, Ying
Zeng, Xiang
Zeng, Yuan‐Shan
Tissue‐Engineered Neural Network Graft Relays Excitatory Signal in the Completely Transected Canine Spinal Cord
title Tissue‐Engineered Neural Network Graft Relays Excitatory Signal in the Completely Transected Canine Spinal Cord
title_full Tissue‐Engineered Neural Network Graft Relays Excitatory Signal in the Completely Transected Canine Spinal Cord
title_fullStr Tissue‐Engineered Neural Network Graft Relays Excitatory Signal in the Completely Transected Canine Spinal Cord
title_full_unstemmed Tissue‐Engineered Neural Network Graft Relays Excitatory Signal in the Completely Transected Canine Spinal Cord
title_short Tissue‐Engineered Neural Network Graft Relays Excitatory Signal in the Completely Transected Canine Spinal Cord
title_sort tissue‐engineered neural network graft relays excitatory signal in the completely transected canine spinal cord
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864506/
https://www.ncbi.nlm.nih.gov/pubmed/31763143
http://dx.doi.org/10.1002/advs.201901240
work_keys_str_mv AT laibiqin tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT chemingtian tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT fengbo tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT baiyurong tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT lige tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT mayuanhuan tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT wanglaijian tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT huangmengyao tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT wangyaqiong tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT jiangbin tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT dingying tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT zengxiang tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord
AT zengyuanshan tissueengineeredneuralnetworkgraftrelaysexcitatorysignalinthecompletelytransectedcaninespinalcord