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Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.

Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter, were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The s...

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Autores principales: Schneider, Yannik K.-H., Ø. Hansen, Kine, Isaksson, Johan, Ullsten, Sara, H. Hansen, Espen, Hammer Andersen, Jeanette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864645/
https://www.ncbi.nlm.nih.gov/pubmed/31694159
http://dx.doi.org/10.3390/molecules24213991
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author Schneider, Yannik K.-H.
Ø. Hansen, Kine
Isaksson, Johan
Ullsten, Sara
H. Hansen, Espen
Hammer Andersen, Jeanette
author_facet Schneider, Yannik K.-H.
Ø. Hansen, Kine
Isaksson, Johan
Ullsten, Sara
H. Hansen, Espen
Hammer Andersen, Jeanette
author_sort Schneider, Yannik K.-H.
collection PubMed
description Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter, were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent.
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spelling pubmed-68646452019-12-23 Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. Schneider, Yannik K.-H. Ø. Hansen, Kine Isaksson, Johan Ullsten, Sara H. Hansen, Espen Hammer Andersen, Jeanette Molecules Article Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter, were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent. MDPI 2019-11-05 /pmc/articles/PMC6864645/ /pubmed/31694159 http://dx.doi.org/10.3390/molecules24213991 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schneider, Yannik K.-H.
Ø. Hansen, Kine
Isaksson, Johan
Ullsten, Sara
H. Hansen, Espen
Hammer Andersen, Jeanette
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
title Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
title_full Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
title_fullStr Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
title_full_unstemmed Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
title_short Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
title_sort anti-bacterial effect and cytotoxicity assessment of lipid 430 isolated from algibacter sp.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864645/
https://www.ncbi.nlm.nih.gov/pubmed/31694159
http://dx.doi.org/10.3390/molecules24213991
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