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Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01
Chitosanase plays an important role in the production of chitooligosaccharides (CHOS), which possess various biological activities. Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, csnB, was cloned from marine bacterium Bacillus sp. BY01 and heterologously expressed in Escheri...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864755/ https://www.ncbi.nlm.nih.gov/pubmed/31671673 http://dx.doi.org/10.3390/molecules24213915 |
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author | Yang, Yue Zheng, Zhou Xiao, Yifei Zhang, Jiaojiao Zhou, Yu Li, Xiao Li, Shangyong Yu, Huiqing |
author_facet | Yang, Yue Zheng, Zhou Xiao, Yifei Zhang, Jiaojiao Zhou, Yu Li, Xiao Li, Shangyong Yu, Huiqing |
author_sort | Yang, Yue |
collection | PubMed |
description | Chitosanase plays an important role in the production of chitooligosaccharides (CHOS), which possess various biological activities. Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, csnB, was cloned from marine bacterium Bacillus sp. BY01 and heterologously expressed in Escherichia coli. The recombinant chitosanase, CsnB, was optimally active at 35 °C and pH 5.0. It was also revealed to be a cold-adapted enzyme, maintaining 39.5% and 40.4% of its maximum activity at 0 and 10 °C, respectively. Meanwhile, CsnB showed wide pH-stability within the range of pH 3.0 to 7.0. Then, an improved reaction condition was built to enhance its thermostability with a final glycerol volume concentration of 20%. Moreover, CsnB was determined to be an endo-type chitosanase, yielding chitosan disaccharides and trisaccharides as the main products. Overall, CsnB provides a new choice for enzymatic CHOS production. |
format | Online Article Text |
id | pubmed-6864755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68647552019-12-23 Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01 Yang, Yue Zheng, Zhou Xiao, Yifei Zhang, Jiaojiao Zhou, Yu Li, Xiao Li, Shangyong Yu, Huiqing Molecules Article Chitosanase plays an important role in the production of chitooligosaccharides (CHOS), which possess various biological activities. Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, csnB, was cloned from marine bacterium Bacillus sp. BY01 and heterologously expressed in Escherichia coli. The recombinant chitosanase, CsnB, was optimally active at 35 °C and pH 5.0. It was also revealed to be a cold-adapted enzyme, maintaining 39.5% and 40.4% of its maximum activity at 0 and 10 °C, respectively. Meanwhile, CsnB showed wide pH-stability within the range of pH 3.0 to 7.0. Then, an improved reaction condition was built to enhance its thermostability with a final glycerol volume concentration of 20%. Moreover, CsnB was determined to be an endo-type chitosanase, yielding chitosan disaccharides and trisaccharides as the main products. Overall, CsnB provides a new choice for enzymatic CHOS production. MDPI 2019-10-30 /pmc/articles/PMC6864755/ /pubmed/31671673 http://dx.doi.org/10.3390/molecules24213915 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Yue Zheng, Zhou Xiao, Yifei Zhang, Jiaojiao Zhou, Yu Li, Xiao Li, Shangyong Yu, Huiqing Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01 |
title | Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01 |
title_full | Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01 |
title_fullStr | Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01 |
title_full_unstemmed | Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01 |
title_short | Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01 |
title_sort | cloning and characterization of a cold-adapted chitosanase from marine bacterium bacillus sp. by01 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864755/ https://www.ncbi.nlm.nih.gov/pubmed/31671673 http://dx.doi.org/10.3390/molecules24213915 |
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