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Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01

Chitosanase plays an important role in the production of chitooligosaccharides (CHOS), which possess various biological activities. Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, csnB, was cloned from marine bacterium Bacillus sp. BY01 and heterologously expressed in Escheri...

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Autores principales: Yang, Yue, Zheng, Zhou, Xiao, Yifei, Zhang, Jiaojiao, Zhou, Yu, Li, Xiao, Li, Shangyong, Yu, Huiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864755/
https://www.ncbi.nlm.nih.gov/pubmed/31671673
http://dx.doi.org/10.3390/molecules24213915
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author Yang, Yue
Zheng, Zhou
Xiao, Yifei
Zhang, Jiaojiao
Zhou, Yu
Li, Xiao
Li, Shangyong
Yu, Huiqing
author_facet Yang, Yue
Zheng, Zhou
Xiao, Yifei
Zhang, Jiaojiao
Zhou, Yu
Li, Xiao
Li, Shangyong
Yu, Huiqing
author_sort Yang, Yue
collection PubMed
description Chitosanase plays an important role in the production of chitooligosaccharides (CHOS), which possess various biological activities. Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, csnB, was cloned from marine bacterium Bacillus sp. BY01 and heterologously expressed in Escherichia coli. The recombinant chitosanase, CsnB, was optimally active at 35 °C and pH 5.0. It was also revealed to be a cold-adapted enzyme, maintaining 39.5% and 40.4% of its maximum activity at 0 and 10 °C, respectively. Meanwhile, CsnB showed wide pH-stability within the range of pH 3.0 to 7.0. Then, an improved reaction condition was built to enhance its thermostability with a final glycerol volume concentration of 20%. Moreover, CsnB was determined to be an endo-type chitosanase, yielding chitosan disaccharides and trisaccharides as the main products. Overall, CsnB provides a new choice for enzymatic CHOS production.
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spelling pubmed-68647552019-12-23 Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01 Yang, Yue Zheng, Zhou Xiao, Yifei Zhang, Jiaojiao Zhou, Yu Li, Xiao Li, Shangyong Yu, Huiqing Molecules Article Chitosanase plays an important role in the production of chitooligosaccharides (CHOS), which possess various biological activities. Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, csnB, was cloned from marine bacterium Bacillus sp. BY01 and heterologously expressed in Escherichia coli. The recombinant chitosanase, CsnB, was optimally active at 35 °C and pH 5.0. It was also revealed to be a cold-adapted enzyme, maintaining 39.5% and 40.4% of its maximum activity at 0 and 10 °C, respectively. Meanwhile, CsnB showed wide pH-stability within the range of pH 3.0 to 7.0. Then, an improved reaction condition was built to enhance its thermostability with a final glycerol volume concentration of 20%. Moreover, CsnB was determined to be an endo-type chitosanase, yielding chitosan disaccharides and trisaccharides as the main products. Overall, CsnB provides a new choice for enzymatic CHOS production. MDPI 2019-10-30 /pmc/articles/PMC6864755/ /pubmed/31671673 http://dx.doi.org/10.3390/molecules24213915 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Yue
Zheng, Zhou
Xiao, Yifei
Zhang, Jiaojiao
Zhou, Yu
Li, Xiao
Li, Shangyong
Yu, Huiqing
Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01
title Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01
title_full Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01
title_fullStr Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01
title_full_unstemmed Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01
title_short Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01
title_sort cloning and characterization of a cold-adapted chitosanase from marine bacterium bacillus sp. by01
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864755/
https://www.ncbi.nlm.nih.gov/pubmed/31671673
http://dx.doi.org/10.3390/molecules24213915
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