Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma

Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from pregnant...

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Autores principales: Vanders, Rebecca L., Hsu, Alan, Gibson, Peter G., Murphy, Vanessa E., Wark, Peter A. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6865028/
https://www.ncbi.nlm.nih.gov/pubmed/31747925
http://dx.doi.org/10.1186/s12931-019-1225-5
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author Vanders, Rebecca L.
Hsu, Alan
Gibson, Peter G.
Murphy, Vanessa E.
Wark, Peter A. B.
author_facet Vanders, Rebecca L.
Hsu, Alan
Gibson, Peter G.
Murphy, Vanessa E.
Wark, Peter A. B.
author_sort Vanders, Rebecca L.
collection PubMed
description Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from pregnant and non-pregnant women with and without asthma. pNECS were infected in vitro with major group Rhinovirus 43 (RV43) and seasonal influenza (H3N2). Following infection, pNECs showed measurable quantities of interferon (IFN)-λ, IL-1β, IL-8, IP-10 and MIP1-α. pNECs provide a safe and effective method for studying respiratory epithelial cell responses during pregnancy.
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spelling pubmed-68650282019-12-12 Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma Vanders, Rebecca L. Hsu, Alan Gibson, Peter G. Murphy, Vanessa E. Wark, Peter A. B. Respir Res Research Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from pregnant and non-pregnant women with and without asthma. pNECS were infected in vitro with major group Rhinovirus 43 (RV43) and seasonal influenza (H3N2). Following infection, pNECs showed measurable quantities of interferon (IFN)-λ, IL-1β, IL-8, IP-10 and MIP1-α. pNECs provide a safe and effective method for studying respiratory epithelial cell responses during pregnancy. BioMed Central 2019-11-20 2019 /pmc/articles/PMC6865028/ /pubmed/31747925 http://dx.doi.org/10.1186/s12931-019-1225-5 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Vanders, Rebecca L.
Hsu, Alan
Gibson, Peter G.
Murphy, Vanessa E.
Wark, Peter A. B.
Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma
title Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma
title_full Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma
title_fullStr Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma
title_full_unstemmed Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma
title_short Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma
title_sort nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6865028/
https://www.ncbi.nlm.nih.gov/pubmed/31747925
http://dx.doi.org/10.1186/s12931-019-1225-5
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