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A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO(2) Derivatives and Its Applications in Bioimaging

Based upon the intramolecular charge transfer (ICT) mechanism, a novel ratiometric fluorescent probe EB was developed to detect SO(3)(2−)/HSO(3)(−). The probe displayed both colorimetric and ratiometric responses toward SO(3)(2−)/HSO(3)(−). It displayed a quick response (within 60 s), good selectivi...

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Autores principales: Qin, Yan-Hong, Jiang, Xiao-Yi, Que, Yuan-Fang, Gu, Jing-Yi, Wu, Tong, Aihemaiti, Ayinazhaer, Shi, Ke-Xin, Kang, Wen-Yu, Hu, Bi-Ying, Lan, Jin-Shuai, Ding, Yue, Zhang, Tong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6865185/
https://www.ncbi.nlm.nih.gov/pubmed/31694349
http://dx.doi.org/10.3390/molecules24214011
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author Qin, Yan-Hong
Jiang, Xiao-Yi
Que, Yuan-Fang
Gu, Jing-Yi
Wu, Tong
Aihemaiti, Ayinazhaer
Shi, Ke-Xin
Kang, Wen-Yu
Hu, Bi-Ying
Lan, Jin-Shuai
Ding, Yue
Zhang, Tong
author_facet Qin, Yan-Hong
Jiang, Xiao-Yi
Que, Yuan-Fang
Gu, Jing-Yi
Wu, Tong
Aihemaiti, Ayinazhaer
Shi, Ke-Xin
Kang, Wen-Yu
Hu, Bi-Ying
Lan, Jin-Shuai
Ding, Yue
Zhang, Tong
author_sort Qin, Yan-Hong
collection PubMed
description Based upon the intramolecular charge transfer (ICT) mechanism, a novel ratiometric fluorescent probe EB was developed to detect SO(3)(2−)/HSO(3)(−). The probe displayed both colorimetric and ratiometric responses toward SO(3)(2−)/HSO(3)(−). It displayed a quick response (within 60 s), good selectivity and high sensitivity (a detection limit of 28 nM) towards SO(3)(2−)/HSO(3)(−). The SO(3)(2−)/HSO(3)(−) sensing mechanism was confirmed as the Michael addition reaction by ESI-MS. Moreover, the probe could be applied to measure the level of sulfite in real samples, like sugar and chrysanthemum, and it could also be used to detect SO(3)(2−)/HSO(3)(−) in HepG2 cells through confocal fluorescence microscopy, which proved its practical application in clinical diagnosis.
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spelling pubmed-68651852019-12-09 A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO(2) Derivatives and Its Applications in Bioimaging Qin, Yan-Hong Jiang, Xiao-Yi Que, Yuan-Fang Gu, Jing-Yi Wu, Tong Aihemaiti, Ayinazhaer Shi, Ke-Xin Kang, Wen-Yu Hu, Bi-Ying Lan, Jin-Shuai Ding, Yue Zhang, Tong Molecules Article Based upon the intramolecular charge transfer (ICT) mechanism, a novel ratiometric fluorescent probe EB was developed to detect SO(3)(2−)/HSO(3)(−). The probe displayed both colorimetric and ratiometric responses toward SO(3)(2−)/HSO(3)(−). It displayed a quick response (within 60 s), good selectivity and high sensitivity (a detection limit of 28 nM) towards SO(3)(2−)/HSO(3)(−). The SO(3)(2−)/HSO(3)(−) sensing mechanism was confirmed as the Michael addition reaction by ESI-MS. Moreover, the probe could be applied to measure the level of sulfite in real samples, like sugar and chrysanthemum, and it could also be used to detect SO(3)(2−)/HSO(3)(−) in HepG2 cells through confocal fluorescence microscopy, which proved its practical application in clinical diagnosis. MDPI 2019-11-05 /pmc/articles/PMC6865185/ /pubmed/31694349 http://dx.doi.org/10.3390/molecules24214011 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qin, Yan-Hong
Jiang, Xiao-Yi
Que, Yuan-Fang
Gu, Jing-Yi
Wu, Tong
Aihemaiti, Ayinazhaer
Shi, Ke-Xin
Kang, Wen-Yu
Hu, Bi-Ying
Lan, Jin-Shuai
Ding, Yue
Zhang, Tong
A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO(2) Derivatives and Its Applications in Bioimaging
title A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO(2) Derivatives and Its Applications in Bioimaging
title_full A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO(2) Derivatives and Its Applications in Bioimaging
title_fullStr A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO(2) Derivatives and Its Applications in Bioimaging
title_full_unstemmed A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO(2) Derivatives and Its Applications in Bioimaging
title_short A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO(2) Derivatives and Its Applications in Bioimaging
title_sort ratiometric and colorimetric hemicyanine fluorescent probe for detection of so(2) derivatives and its applications in bioimaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6865185/
https://www.ncbi.nlm.nih.gov/pubmed/31694349
http://dx.doi.org/10.3390/molecules24214011
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