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Primary myogenesis in the sand lizard (Lacerta agilis) limb bud

Our studies conducted on reptilian limb muscle development revealed, for the first time, early forelimb muscle differentiation at the morphological and molecular level. Sand lizard skeletal muscle differentiation in the early forelimb bud was investigated by light, confocal, and transmission electro...

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Autores principales: Lewandowski, Damian, Dubińska-Magiera, Magda, Garbiec, Arnold, Daczewska, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6867991/
https://www.ncbi.nlm.nih.gov/pubmed/31214772
http://dx.doi.org/10.1007/s00427-019-00635-7
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author Lewandowski, Damian
Dubińska-Magiera, Magda
Garbiec, Arnold
Daczewska, Małgorzata
author_facet Lewandowski, Damian
Dubińska-Magiera, Magda
Garbiec, Arnold
Daczewska, Małgorzata
author_sort Lewandowski, Damian
collection PubMed
description Our studies conducted on reptilian limb muscle development revealed, for the first time, early forelimb muscle differentiation at the morphological and molecular level. Sand lizard skeletal muscle differentiation in the early forelimb bud was investigated by light, confocal, and transmission electron microscopy as well as western blot. The early forelimb bud, filled with mesenchymal cells, is surrounded by monolayer epithelium cells. The immunocytochemical analysis revealed the presence of Pax3- and Lbx-positive cells in the vicinity of the ventro-lateral lip (VLL) of the dermomyotome, suggesting that VLL is the source of limb muscle progenitor cells. Furthermore, Pax3- and Lbx-positive cells were observed in the dorsal and ventral myogenic pools of the forelimb bud. Skeletal muscle development in the early limb bud is asynchronous, which is manifested by the presence of myogenic cells in different stages of differentiation: multinucleated myotubes with well-developed contractile apparatus, myoblasts, and mitotically active premyoblasts. The western blot analysis revealed the presence of MyoD and Myf5 proteins in all investigated developmental stages. The MyoD western blot analysis showed two bands corresponding to monomeric (mMyoD) and dimeric (dMyoD) fractions. Two separate bands were also detected in the case of Myf5. The observed bands were related to non-phosphorylated (Myf5) and phosphorylated (pMyf5) fractions of Myf5. Our investigations on sand lizard forelimb myogenesis showed that the pattern of muscle differentiation in the early forelimb bud shares many features with rodents and chicks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00427-019-00635-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-68679912019-12-05 Primary myogenesis in the sand lizard (Lacerta agilis) limb bud Lewandowski, Damian Dubińska-Magiera, Magda Garbiec, Arnold Daczewska, Małgorzata Dev Genes Evol Original Article Our studies conducted on reptilian limb muscle development revealed, for the first time, early forelimb muscle differentiation at the morphological and molecular level. Sand lizard skeletal muscle differentiation in the early forelimb bud was investigated by light, confocal, and transmission electron microscopy as well as western blot. The early forelimb bud, filled with mesenchymal cells, is surrounded by monolayer epithelium cells. The immunocytochemical analysis revealed the presence of Pax3- and Lbx-positive cells in the vicinity of the ventro-lateral lip (VLL) of the dermomyotome, suggesting that VLL is the source of limb muscle progenitor cells. Furthermore, Pax3- and Lbx-positive cells were observed in the dorsal and ventral myogenic pools of the forelimb bud. Skeletal muscle development in the early limb bud is asynchronous, which is manifested by the presence of myogenic cells in different stages of differentiation: multinucleated myotubes with well-developed contractile apparatus, myoblasts, and mitotically active premyoblasts. The western blot analysis revealed the presence of MyoD and Myf5 proteins in all investigated developmental stages. The MyoD western blot analysis showed two bands corresponding to monomeric (mMyoD) and dimeric (dMyoD) fractions. Two separate bands were also detected in the case of Myf5. The observed bands were related to non-phosphorylated (Myf5) and phosphorylated (pMyf5) fractions of Myf5. Our investigations on sand lizard forelimb myogenesis showed that the pattern of muscle differentiation in the early forelimb bud shares many features with rodents and chicks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00427-019-00635-7) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-06-18 2019 /pmc/articles/PMC6867991/ /pubmed/31214772 http://dx.doi.org/10.1007/s00427-019-00635-7 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Lewandowski, Damian
Dubińska-Magiera, Magda
Garbiec, Arnold
Daczewska, Małgorzata
Primary myogenesis in the sand lizard (Lacerta agilis) limb bud
title Primary myogenesis in the sand lizard (Lacerta agilis) limb bud
title_full Primary myogenesis in the sand lizard (Lacerta agilis) limb bud
title_fullStr Primary myogenesis in the sand lizard (Lacerta agilis) limb bud
title_full_unstemmed Primary myogenesis in the sand lizard (Lacerta agilis) limb bud
title_short Primary myogenesis in the sand lizard (Lacerta agilis) limb bud
title_sort primary myogenesis in the sand lizard (lacerta agilis) limb bud
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6867991/
https://www.ncbi.nlm.nih.gov/pubmed/31214772
http://dx.doi.org/10.1007/s00427-019-00635-7
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